An integrated workflow for charting the human interaction proteome: insights into the PP2A system
Abstract Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP‐MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2009-01-01
|
| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2008.75 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849225742758445056 |
|---|---|
| author | Timo Glatter Alexander Wepf Ruedi Aebersold Matthias Gstaiger |
| author_facet | Timo Glatter Alexander Wepf Ruedi Aebersold Matthias Gstaiger |
| author_sort | Timo Glatter |
| collection | DOAJ |
| description | Abstract Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP‐MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP‐MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP‐MS studies on human protein complexes. |
| format | Article |
| id | doaj-art-0bdef0c2f00342f8ade8e4e8a7705cf7 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-0bdef0c2f00342f8ade8e4e8a7705cf72025-08-24T11:58:58ZengSpringer NatureMolecular Systems Biology1744-42922009-01-015111410.1038/msb.2008.75An integrated workflow for charting the human interaction proteome: insights into the PP2A systemTimo Glatter0Alexander Wepf1Ruedi Aebersold2Matthias Gstaiger3Institute of Molecular Systems Biology, ETH ZurichInstitute of Molecular Systems Biology, ETH ZurichInstitute of Molecular Systems Biology, ETH ZurichInstitute of Molecular Systems Biology, ETH ZurichAbstract Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP‐MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP‐MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP‐MS studies on human protein complexes.https://doi.org/10.1038/msb.2008.75human interactomemass spectrometryPP2Aprotein complexes |
| spellingShingle | Timo Glatter Alexander Wepf Ruedi Aebersold Matthias Gstaiger An integrated workflow for charting the human interaction proteome: insights into the PP2A system Molecular Systems Biology human interactome mass spectrometry PP2A protein complexes |
| title | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
| title_full | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
| title_fullStr | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
| title_full_unstemmed | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
| title_short | An integrated workflow for charting the human interaction proteome: insights into the PP2A system |
| title_sort | integrated workflow for charting the human interaction proteome insights into the pp2a system |
| topic | human interactome mass spectrometry PP2A protein complexes |
| url | https://doi.org/10.1038/msb.2008.75 |
| work_keys_str_mv | AT timoglatter anintegratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem AT alexanderwepf anintegratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem AT ruediaebersold anintegratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem AT matthiasgstaiger anintegratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem AT timoglatter integratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem AT alexanderwepf integratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem AT ruediaebersold integratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem AT matthiasgstaiger integratedworkflowforchartingthehumaninteractionproteomeinsightsintothepp2asystem |