Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis
ABSTRACT: Breastfeeding by mothers with gestational diabetes mellitus (GDM) has been shown to reduce maternal insulin demands and diminish the risks of diabetes in infants, leading to improved long-term health outcomes. Milk fat globule membrane (MFGM) proteins play a crucial role in influencing the...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224010725 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850266405005426688 |
|---|---|
| author | Ye Tao Qingcheng Wang Min Xiao Haihong Li Haifeng Wang Zhujun Mao Lai Zhang XiaoLi Zhou Huijuan Yang Qing Shen |
| author_facet | Ye Tao Qingcheng Wang Min Xiao Haihong Li Haifeng Wang Zhujun Mao Lai Zhang XiaoLi Zhou Huijuan Yang Qing Shen |
| author_sort | Ye Tao |
| collection | DOAJ |
| description | ABSTRACT: Breastfeeding by mothers with gestational diabetes mellitus (GDM) has been shown to reduce maternal insulin demands and diminish the risks of diabetes in infants, leading to improved long-term health outcomes. Milk fat globule membrane (MFGM) proteins play a crucial role in influencing the immunity and cognitive development of infants. Understanding the alterations in MFGM proteins in breast milk from mothers with GDM is essential for enhancing their self-efficacy and increase breastfeeding rates. The objective of this study is to investigate and compare MFGM proteins in milk from mothers with and without GDM based on tandem mass tag (TMT) labeling and liquid chromatography–tandem MS techniques. A total of 5,402 proteins were identified, including 4 upregulated proteins and 24 downregulated proteins. These significantly altered proteins were found to be associated with human diseases, cellular processes, and metabolism pathways. Additionally, the oxidative phosphorylation pathway emerged as the predominant pathway through Gene Set Enrichment Analysis involving all genes. |
| format | Article |
| id | doaj-art-0bde807905934200ac9f8d8ec4cc550a |
| institution | OA Journals |
| issn | 0022-0302 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-0bde807905934200ac9f8d8ec4cc550a2025-08-20T01:54:11ZengElsevierJournal of Dairy Science0022-03022024-12-0110712103401035110.3168/jds.2024-25077Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysisYe Tao0Qingcheng Wang1Min Xiao2Haihong Li3Haifeng Wang4Zhujun Mao5Lai Zhang6XiaoLi Zhou7Huijuan Yang8Qing Shen9Hangzhou Linping District Maternal & Child Health Care Hospital, Hangzhou, Zhejiang 311113, ChinaLaboratory of Medicine-Food Homology Innovation and Achievement Transformation, Linping Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, Zhejiang 311110, ChinaHangzhou Linping District Maternal & Child Health Care Hospital, Hangzhou, Zhejiang 311113, ChinaHangzhou Linping District Maternal & Child Health Care Hospital, Hangzhou, Zhejiang 311113, ChinaLaboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China; Corresponding authorsPanvascular Diseases Research Center, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, ChinaHangzhou Linping District Maternal & Child Health Care Hospital, Hangzhou, Zhejiang 311113, ChinaHangzhou Linping District Maternal & Child Health Care Hospital, Hangzhou, Zhejiang 311113, ChinaCollege of Standardization, China Jiliang University, Hangzhou 310018, PR ChinaLaboratory of Medicine-Food Homology Innovation and Achievement Transformation, Linping Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, Zhejiang 311110, China; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China; Panvascular Diseases Research Center, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China; Corresponding authorsABSTRACT: Breastfeeding by mothers with gestational diabetes mellitus (GDM) has been shown to reduce maternal insulin demands and diminish the risks of diabetes in infants, leading to improved long-term health outcomes. Milk fat globule membrane (MFGM) proteins play a crucial role in influencing the immunity and cognitive development of infants. Understanding the alterations in MFGM proteins in breast milk from mothers with GDM is essential for enhancing their self-efficacy and increase breastfeeding rates. The objective of this study is to investigate and compare MFGM proteins in milk from mothers with and without GDM based on tandem mass tag (TMT) labeling and liquid chromatography–tandem MS techniques. A total of 5,402 proteins were identified, including 4 upregulated proteins and 24 downregulated proteins. These significantly altered proteins were found to be associated with human diseases, cellular processes, and metabolism pathways. Additionally, the oxidative phosphorylation pathway emerged as the predominant pathway through Gene Set Enrichment Analysis involving all genes.http://www.sciencedirect.com/science/article/pii/S0022030224010725gestational diabetes mellitusmilk fat globule membranetandem mass tag labeling techniquesgene set enrichment analysis |
| spellingShingle | Ye Tao Qingcheng Wang Min Xiao Haihong Li Haifeng Wang Zhujun Mao Lai Zhang XiaoLi Zhou Huijuan Yang Qing Shen Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis Journal of Dairy Science gestational diabetes mellitus milk fat globule membrane tandem mass tag labeling techniques gene set enrichment analysis |
| title | Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis |
| title_full | Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis |
| title_fullStr | Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis |
| title_full_unstemmed | Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis |
| title_short | Gestational diabetes mellitus-induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis |
| title_sort | gestational diabetes mellitus induced milk fat globule membrane protein changes of human mature milk based on tandem mass tag proteomic analysis |
| topic | gestational diabetes mellitus milk fat globule membrane tandem mass tag labeling techniques gene set enrichment analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224010725 |
| work_keys_str_mv | AT yetao gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT qingchengwang gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT minxiao gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT haihongli gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT haifengwang gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT zhujunmao gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT laizhang gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT xiaolizhou gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT huijuanyang gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis AT qingshen gestationaldiabetesmellitusinducedmilkfatglobulemembraneproteinchangesofhumanmaturemilkbasedontandemmasstagproteomicanalysis |