Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial Fungi
<i>Lysobacter</i>, a genus of Gram-negative bacteria, is known for producing antibiotic compounds, making it a promising biocontrol agent against crop pathogens. As part of the soil microbiome, <i>Lysobacter</i> species cooccur with a variety of microorganisms in the ecosyste...
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2025-05-01
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| author | Vishakha Jayasekera Yong Han Liangcheng Du |
| author_facet | Vishakha Jayasekera Yong Han Liangcheng Du |
| author_sort | Vishakha Jayasekera |
| collection | DOAJ |
| description | <i>Lysobacter</i>, a genus of Gram-negative bacteria, is known for producing antibiotic compounds, making it a promising biocontrol agent against crop pathogens. As part of the soil microbiome, <i>Lysobacter</i> species cooccur with a variety of microorganisms in the ecosystem. However, little is known about bioactive natural products involved in <i>Lysobacter</i>’s interactions with other organisms. This study investigated interactions between <i>Lysobacter</i> sp. 3655 and two economically important fungal pathogens, <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>. We discovered a <i>Lysobacter</i> molecule that is dramatically suppressed when co-culturing with the fungi, and the structure of this molecule was determined to be pyrrole-2-carboxylic acid (P2C). Chitin, a primary component of fungal cell walls, also suppressed P2C production in <i>Lysobacter</i>. Exogenous P2C addition promoted formation of <i>Lysobacter</i> biofilms within a range of concentrations, suggesting its potential role as a signaling molecule. Previously reported result showed that the mutation of the global regulator Clp in <i>Lysobacter enzymogenes</i> led to drastic increase of biofilm formation. Intriguingly, while P2C increased the biofilm formation in the wildtype of <i>L. enzymogenes</i>, it reduced the biofilms in the Clp mutant. Together, these findings reveal P2C as a novel signaling molecule mediating the interaction between <i>Lysobacter</i> and surrounding fungal species, highlighting its role in <i>Lysobacter</i> adaptation in response to environmental conditions. |
| format | Article |
| id | doaj-art-0bdccbfc0d65460686dd3208bcfbe925 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Microorganisms |
| spelling | doaj-art-0bdccbfc0d65460686dd3208bcfbe9252025-08-20T03:27:32ZengMDPI AGMicroorganisms2076-26072025-05-01136120210.3390/microorganisms13061202Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial FungiVishakha Jayasekera0Yong Han1Liangcheng Du2Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USAEdison Biotechnology Institute, Ohio University, Athens, OH 45701, USADepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA<i>Lysobacter</i>, a genus of Gram-negative bacteria, is known for producing antibiotic compounds, making it a promising biocontrol agent against crop pathogens. As part of the soil microbiome, <i>Lysobacter</i> species cooccur with a variety of microorganisms in the ecosystem. However, little is known about bioactive natural products involved in <i>Lysobacter</i>’s interactions with other organisms. This study investigated interactions between <i>Lysobacter</i> sp. 3655 and two economically important fungal pathogens, <i>Fusarium graminearum</i> and <i>Fusarium verticillioides</i>. We discovered a <i>Lysobacter</i> molecule that is dramatically suppressed when co-culturing with the fungi, and the structure of this molecule was determined to be pyrrole-2-carboxylic acid (P2C). Chitin, a primary component of fungal cell walls, also suppressed P2C production in <i>Lysobacter</i>. Exogenous P2C addition promoted formation of <i>Lysobacter</i> biofilms within a range of concentrations, suggesting its potential role as a signaling molecule. Previously reported result showed that the mutation of the global regulator Clp in <i>Lysobacter enzymogenes</i> led to drastic increase of biofilm formation. Intriguingly, while P2C increased the biofilm formation in the wildtype of <i>L. enzymogenes</i>, it reduced the biofilms in the Clp mutant. Together, these findings reveal P2C as a novel signaling molecule mediating the interaction between <i>Lysobacter</i> and surrounding fungal species, highlighting its role in <i>Lysobacter</i> adaptation in response to environmental conditions.https://www.mdpi.com/2076-2607/13/6/1202<i>Lysobacter</i>biocontrolpyrrole-2-carboxylic acidsignalingbiofilm |
| spellingShingle | Vishakha Jayasekera Yong Han Liangcheng Du Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial Fungi Microorganisms <i>Lysobacter</i> biocontrol pyrrole-2-carboxylic acid signaling biofilm |
| title | Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial Fungi |
| title_full | Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial Fungi |
| title_fullStr | Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial Fungi |
| title_full_unstemmed | Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial Fungi |
| title_short | Identification of Pyrrole-2-Carboxylic Acid from the Biocontrol Agent <i>Lysobacter</i> Involved in Interactions with Fusarial Fungi |
| title_sort | identification of pyrrole 2 carboxylic acid from the biocontrol agent i lysobacter i involved in interactions with fusarial fungi |
| topic | <i>Lysobacter</i> biocontrol pyrrole-2-carboxylic acid signaling biofilm |
| url | https://www.mdpi.com/2076-2607/13/6/1202 |
| work_keys_str_mv | AT vishakhajayasekera identificationofpyrrole2carboxylicacidfromthebiocontrolagentilysobacteriinvolvedininteractionswithfusarialfungi AT yonghan identificationofpyrrole2carboxylicacidfromthebiocontrolagentilysobacteriinvolvedininteractionswithfusarialfungi AT liangchengdu identificationofpyrrole2carboxylicacidfromthebiocontrolagentilysobacteriinvolvedininteractionswithfusarialfungi |