Identification of potential substrates of LNX1 in chicken cells

Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin ligase,...

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Main Authors: Yuhong Liu, Qianmei Gao, Qi Zhang, Chen Li, Sha Liu, Meng Su, Danli Song, Guiping Zhao, Qinghe Li
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579124012112
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author Yuhong Liu
Qianmei Gao
Qi Zhang
Chen Li
Sha Liu
Meng Su
Danli Song
Guiping Zhao
Qinghe Li
author_facet Yuhong Liu
Qianmei Gao
Qi Zhang
Chen Li
Sha Liu
Meng Su
Danli Song
Guiping Zhao
Qinghe Li
author_sort Yuhong Liu
collection DOAJ
description Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin ligase, catalyzes the ubiquitination and subsequent degradation of specific substrate proteins. A cell line with stable knockdown of the LNX1 gene was developed using chicken macrophages (HD11) as the model. The relative quantitative values of each protein were analyzed using 4D-FastDIA quantitative proteomics. This identified 319 proteins with up-regulated expression, a majority of which relate to the immune system. Overall, this study uncovered potential substrate proteins targeted by LNX1 and their associated biological pathways. The findings suggest that LNX1 could be implicated in the regulation of the chicken innate immune system by mediating protein ubiquitination.
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institution Kabale University
issn 0032-5791
language English
publishDate 2025-01-01
publisher Elsevier
record_format Article
series Poultry Science
spelling doaj-art-0bcca6783ce34d118fa8c27d5dbe31652025-01-22T05:40:46ZengElsevierPoultry Science0032-57912025-01-011041104633Identification of potential substrates of LNX1 in chicken cellsYuhong Liu0Qianmei Gao1Qi Zhang2Chen Li3Sha Liu4Meng Su5Danli Song6Guiping Zhao7Qinghe Li8Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaInstitute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Corresponding author.Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin ligase, catalyzes the ubiquitination and subsequent degradation of specific substrate proteins. A cell line with stable knockdown of the LNX1 gene was developed using chicken macrophages (HD11) as the model. The relative quantitative values of each protein were analyzed using 4D-FastDIA quantitative proteomics. This identified 319 proteins with up-regulated expression, a majority of which relate to the immune system. Overall, this study uncovered potential substrate proteins targeted by LNX1 and their associated biological pathways. The findings suggest that LNX1 could be implicated in the regulation of the chicken innate immune system by mediating protein ubiquitination.http://www.sciencedirect.com/science/article/pii/S0032579124012112HD11LNX1UbiquitinationProteomicsInnate Immunity
spellingShingle Yuhong Liu
Qianmei Gao
Qi Zhang
Chen Li
Sha Liu
Meng Su
Danli Song
Guiping Zhao
Qinghe Li
Identification of potential substrates of LNX1 in chicken cells
Poultry Science
HD11
LNX1
Ubiquitination
Proteomics
Innate Immunity
title Identification of potential substrates of LNX1 in chicken cells
title_full Identification of potential substrates of LNX1 in chicken cells
title_fullStr Identification of potential substrates of LNX1 in chicken cells
title_full_unstemmed Identification of potential substrates of LNX1 in chicken cells
title_short Identification of potential substrates of LNX1 in chicken cells
title_sort identification of potential substrates of lnx1 in chicken cells
topic HD11
LNX1
Ubiquitination
Proteomics
Innate Immunity
url http://www.sciencedirect.com/science/article/pii/S0032579124012112
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