Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism
In Thermococcales, energy metabolism genes are regulated by the sulfur-responsive transcriptional regulator SurR. In the piezophilic archaeon Thermococcus barophilus, these genes are also influenced by hydrostatic pressure. To explore the interaction between pressure, sulfur availability, and gene r...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1593936/full |
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| author | Yann Moalic Toan Bao Hung Nguyen Jordan Hartunians Tiphaine Birien Axel Thiel Mohamed Jebbar |
| author_facet | Yann Moalic Toan Bao Hung Nguyen Jordan Hartunians Tiphaine Birien Axel Thiel Mohamed Jebbar |
| author_sort | Yann Moalic |
| collection | DOAJ |
| description | In Thermococcales, energy metabolism genes are regulated by the sulfur-responsive transcriptional regulator SurR. In the piezophilic archaeon Thermococcus barophilus, these genes are also influenced by hydrostatic pressure. To explore the interaction between pressure, sulfur availability, and gene regulation, we constructed and analyzed several deletion mutants, including a partial surR knockout, under varying conditions. Our results show that hydrostatic pressure modulates the expression of energy metabolism genes and that SurR is essential for activating the hydrogenogenic gene cluster, even in sulfur-rich environments. Under sulfur limitation, the membrane-bound sulfur-reducing complex (MBS) is required for normal growth. These regulatory patterns expand current models derived from non-piezophilic species such as Pyrococcus furiosus and Thermococcus kodakarensis. Overall, our findings indicate that hydrostatic pressure shapes SurR function in T. barophilus, reflecting its adaptive plasticity in extreme environments. |
| format | Article |
| id | doaj-art-0bc8e5a45fb94853ad3a7ea27da917a7 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-0bc8e5a45fb94853ad3a7ea27da917a72025-08-20T03:21:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15939361593936Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolismYann MoalicToan Bao Hung NguyenJordan HartuniansTiphaine BirienAxel ThielMohamed JebbarIn Thermococcales, energy metabolism genes are regulated by the sulfur-responsive transcriptional regulator SurR. In the piezophilic archaeon Thermococcus barophilus, these genes are also influenced by hydrostatic pressure. To explore the interaction between pressure, sulfur availability, and gene regulation, we constructed and analyzed several deletion mutants, including a partial surR knockout, under varying conditions. Our results show that hydrostatic pressure modulates the expression of energy metabolism genes and that SurR is essential for activating the hydrogenogenic gene cluster, even in sulfur-rich environments. Under sulfur limitation, the membrane-bound sulfur-reducing complex (MBS) is required for normal growth. These regulatory patterns expand current models derived from non-piezophilic species such as Pyrococcus furiosus and Thermococcus kodakarensis. Overall, our findings indicate that hydrostatic pressure shapes SurR function in T. barophilus, reflecting its adaptive plasticity in extreme environments.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1593936/fullpiezophilic archaeahigh hydrostatic pressureenergy metabolismtranscriptional regulationsulfur metabolismgene expression |
| spellingShingle | Yann Moalic Toan Bao Hung Nguyen Jordan Hartunians Tiphaine Birien Axel Thiel Mohamed Jebbar Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism Frontiers in Microbiology piezophilic archaea high hydrostatic pressure energy metabolism transcriptional regulation sulfur metabolism gene expression |
| title | Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism |
| title_full | Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism |
| title_fullStr | Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism |
| title_full_unstemmed | Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism |
| title_short | Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism |
| title_sort | regulation of gene expression under high hydrostatic pressure the versatile role of the master regulator surr in energy metabolism |
| topic | piezophilic archaea high hydrostatic pressure energy metabolism transcriptional regulation sulfur metabolism gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1593936/full |
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