Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The Gambia

Background and Aim: In The Gambia, malaria transmission persists due to insecticide resistance and residual vector behavior, despite extensive use of indoor residual spraying and insecticide-treated nets. Community-led larval source management using Bacillus thuringiensis var. israelensis (Bti) offe...

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Main Authors: Babucarr Jassey, Ririh Yudhastuti, Buba Manjang, Ibrahim Touray, Muhammad Rasyid Ridha, Khuliyah Candraning Diyanah, Fitiara Indah Permatasari
Format: Article
Language:English
Published: Veterinary World 2025-08-01
Series:Veterinary World
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Online Access:https://www.veterinaryworld.org/Vol.18/August-2025/1.pdf
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author Babucarr Jassey
Ririh Yudhastuti
Buba Manjang
Ibrahim Touray
Muhammad Rasyid Ridha
Khuliyah Candraning Diyanah
Fitiara Indah Permatasari
author_facet Babucarr Jassey
Ririh Yudhastuti
Buba Manjang
Ibrahim Touray
Muhammad Rasyid Ridha
Khuliyah Candraning Diyanah
Fitiara Indah Permatasari
author_sort Babucarr Jassey
collection DOAJ
description Background and Aim: In The Gambia, malaria transmission persists due to insecticide resistance and residual vector behavior, despite extensive use of indoor residual spraying and insecticide-treated nets. Community-led larval source management using Bacillus thuringiensis var. israelensis (Bti) offers a sustainable vector control alternative. This study aimed to assess the effectiveness and feasibility of community-led Bti application for reducing Anopheles mosquito populations, compared to expert-supervised application and non-intervention control arms. Materials and Methods: A non-randomized controlled trial was conducted across malaria-endemic regions in The Gambia from 2023 to 2024. Intervention arms included: (1) community-led Bti application, (2) expert-supervised Bti application, and (3) untreated control. Trained volunteers and entomologists applied Bti to breeding sites at weekly or biweekly intervals. Entomological surveys were conducted biweekly to monitor larval, pupal, and adult mosquito densities. Data were analyzed using generalized linear mixed models and negative binomial regression, adjusting for environmental covariates. Results: By round 10, community-led and expert-supervised interventions achieved 96.8% and 98.6% reductions in larval density, 97.4% and 99.1% reductions in pupal emergence, and 96.2% and 98.8% reductions in adult mosquito populations, respectively. Statistically significant declines in mosquito densities were observed by 2024 (p < 0.001). Community participation enabled high coverage and operational sustainability, with over 85% of participants reporting visible mosquito reduction. Conclusion: Seasonal Bti application, especially when led by trained community members, significantly suppresses Anopheles populations. Although expert-supervised methods yielded slightly higher efficacy, community-led biolarviciding offers a scalable, sustainable, and environmentally safe vector control strategy, supporting The Gambia’s malaria elimination goals.
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spelling doaj-art-0bba5f3326234df285e9acd57c6f1ccc2025-08-20T02:56:20ZengVeterinary WorldVeterinary World0972-89882231-09162025-08-011872158216810.14202/vetworld.2025.2158-2168Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The GambiaBabucarr Jassey0https://orcid.org/0000-0001-5913-858XRirih Yudhastuti1https://orcid.org/0000-0002-1091-0687Buba Manjang2https://orcid.org/0000-0003-2700-5875Ibrahim Touray3https://orcid.org/0009-0006-4792-5061Muhammad Rasyid Ridha4https://orcid.org/0000-0003-3164-336XKhuliyah Candraning Diyanah5https://orcid.org/0009-0009-2651-5233Fitiara Indah Permatasari6https://orcid.org/0009-0006-5936-7319Department of Public Health, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia.Department of Public Health, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia.Department of Public Health Services, Ministry of Health, The Gambia, West Africa.Department of Public Health Services, Ministry of Health, The Gambia, West Africa.Department of Public Health, Faculty of Medicine and Health Sciences, Universitas Lambung Mangkurat, Banjarbaru, Indonesia.Department of Public Health, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia.Department of Nutrition, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia.Background and Aim: In The Gambia, malaria transmission persists due to insecticide resistance and residual vector behavior, despite extensive use of indoor residual spraying and insecticide-treated nets. Community-led larval source management using Bacillus thuringiensis var. israelensis (Bti) offers a sustainable vector control alternative. This study aimed to assess the effectiveness and feasibility of community-led Bti application for reducing Anopheles mosquito populations, compared to expert-supervised application and non-intervention control arms. Materials and Methods: A non-randomized controlled trial was conducted across malaria-endemic regions in The Gambia from 2023 to 2024. Intervention arms included: (1) community-led Bti application, (2) expert-supervised Bti application, and (3) untreated control. Trained volunteers and entomologists applied Bti to breeding sites at weekly or biweekly intervals. Entomological surveys were conducted biweekly to monitor larval, pupal, and adult mosquito densities. Data were analyzed using generalized linear mixed models and negative binomial regression, adjusting for environmental covariates. Results: By round 10, community-led and expert-supervised interventions achieved 96.8% and 98.6% reductions in larval density, 97.4% and 99.1% reductions in pupal emergence, and 96.2% and 98.8% reductions in adult mosquito populations, respectively. Statistically significant declines in mosquito densities were observed by 2024 (p < 0.001). Community participation enabled high coverage and operational sustainability, with over 85% of participants reporting visible mosquito reduction. Conclusion: Seasonal Bti application, especially when led by trained community members, significantly suppresses Anopheles populations. Although expert-supervised methods yielded slightly higher efficacy, community-led biolarviciding offers a scalable, sustainable, and environmentally safe vector control strategy, supporting The Gambia’s malaria elimination goals.https://www.veterinaryworld.org/Vol.18/August-2025/1.pdfanopheles mosquitoesbacillus thuringiensis israelensisbiolarvicidingcommunity participationlarval source managementmalaria controlthe gambia
spellingShingle Babucarr Jassey
Ririh Yudhastuti
Buba Manjang
Ibrahim Touray
Muhammad Rasyid Ridha
Khuliyah Candraning Diyanah
Fitiara Indah Permatasari
Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The Gambia
Veterinary World
anopheles mosquitoes
bacillus thuringiensis israelensis
biolarviciding
community participation
larval source management
malaria control
the gambia
title Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The Gambia
title_full Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The Gambia
title_fullStr Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The Gambia
title_full_unstemmed Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The Gambia
title_short Empowering communities for malaria control: Effectiveness of community-led biolarviciding using Bacillus thuringiensis israelensis in The Gambia
title_sort empowering communities for malaria control effectiveness of community led biolarviciding using bacillus thuringiensis israelensis in the gambia
topic anopheles mosquitoes
bacillus thuringiensis israelensis
biolarviciding
community participation
larval source management
malaria control
the gambia
url https://www.veterinaryworld.org/Vol.18/August-2025/1.pdf
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