An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section
Relative abundance of tumour angiogenesis has been shown to be of clinical relevance in cancers of various locations such as the ovary. Nevertheless, several problems are encountered when quantifying tumour microvessels: (i) as many other tumour markers, vascularity pattern is often heterogeneous wi...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2003/473902 |
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| _version_ | 1849308948799160320 |
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| author | Nga Tran Kim Nicolas Elie Benoît Plancoulaine Paulette Herlin Michel Coster |
| author_facet | Nga Tran Kim Nicolas Elie Benoît Plancoulaine Paulette Herlin Michel Coster |
| author_sort | Nga Tran Kim |
| collection | DOAJ |
| description | Relative abundance of tumour angiogenesis has been shown to be of clinical relevance in cancers of various locations such as the ovary. Nevertheless, several problems are encountered when quantifying tumour microvessels: (i) as many other tumour markers, vascularity pattern is often heterogeneous within the tumour mass and even within the same histological section. As a consequence, an adequate acquisition method must be developed for accurate field sampling. (ii) Manual microvessel counting is long, tedious and subject to poor reproducibility. Introduction in routine practice requires a fast, reproducible and reliable automatic image processing. In this study we present an original procedure combining a slide scanner image acquisition and a fully automatic image analysis sequence. The slide scanner offers the advantage of recording an image of the whole histological section for subsequent automatic blood vessel detection and hot spot area location. Microvessel density and surface fraction were measured for the whole section as well as within hot spots. Different immunostaining methods were tested in order to optimise the procedure. Moreover, the method proposed was submitted to a quality control procedure, with reference to interactive identification of microvessels at scanner level. This experiment showed that 93 to 97% of blood vessels were detected, according to the staining protocol used. Colour figures can be viewed on http://www.esacp.org/acp/2003/25‐2/kim.htm. |
| format | Article |
| id | doaj-art-0bb9c3dfcb5b4cbe9eeb20ea7601f67c |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-0bb9c3dfcb5b4cbe9eeb20ea7601f67c2025-08-20T03:54:19ZengWileyAnalytical Cellular Pathology0921-89121878-36512003-01-01252637510.1155/2003/473902An Original Approach for Quantification of Blood Vessels on the Whole Tumour SectionNga Tran Kim0Nicolas Elie1Benoît Plancoulaine2Paulette Herlin3Michel Coster4GRECAN, Equipe Universitaire d'Accueil 1772, Centre F. Baclesse, Route de Lion sur Mer, F‐14076 Caen, FranceGRECAN, Equipe Universitaire d'Accueil 1772, Centre F. Baclesse, Route de Lion sur Mer, F‐14076 Caen, FranceDépartement de Mesures Physiques, IUT de Caen, Boulevard du Maréchal Juin, F‐14032 Caen, FranceGRECAN, Equipe Universitaire d'Accueil 1772, Centre F. Baclesse, Route de Lion sur Mer, F‐14076 Caen, FranceLERMAT‐ISMRA, UPRES‐A CNRS 6004, F‐14032 Caen, FranceRelative abundance of tumour angiogenesis has been shown to be of clinical relevance in cancers of various locations such as the ovary. Nevertheless, several problems are encountered when quantifying tumour microvessels: (i) as many other tumour markers, vascularity pattern is often heterogeneous within the tumour mass and even within the same histological section. As a consequence, an adequate acquisition method must be developed for accurate field sampling. (ii) Manual microvessel counting is long, tedious and subject to poor reproducibility. Introduction in routine practice requires a fast, reproducible and reliable automatic image processing. In this study we present an original procedure combining a slide scanner image acquisition and a fully automatic image analysis sequence. The slide scanner offers the advantage of recording an image of the whole histological section for subsequent automatic blood vessel detection and hot spot area location. Microvessel density and surface fraction were measured for the whole section as well as within hot spots. Different immunostaining methods were tested in order to optimise the procedure. Moreover, the method proposed was submitted to a quality control procedure, with reference to interactive identification of microvessels at scanner level. This experiment showed that 93 to 97% of blood vessels were detected, according to the staining protocol used. Colour figures can be viewed on http://www.esacp.org/acp/2003/25‐2/kim.htm.http://dx.doi.org/10.1155/2003/473902 |
| spellingShingle | Nga Tran Kim Nicolas Elie Benoît Plancoulaine Paulette Herlin Michel Coster An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section Analytical Cellular Pathology |
| title | An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section |
| title_full | An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section |
| title_fullStr | An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section |
| title_full_unstemmed | An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section |
| title_short | An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section |
| title_sort | original approach for quantification of blood vessels on the whole tumour section |
| url | http://dx.doi.org/10.1155/2003/473902 |
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