The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United Kingdom
An EBSL-encoding plasmid, pESBL-PH, was identified during a nosocomial outbreak of <i>Klebsiella pneumoniae</i> ST628 at a United Kingdom general district hospital in 2018. The plasmid from the earliest 2018 <i>K. pneumoniae</i> strain discovered during the outbreak was assem...
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2025-04-01
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| author | Stephen Mark Edward Fordham Anna Mantzouratou Elizabeth Sheridan |
| author_facet | Stephen Mark Edward Fordham Anna Mantzouratou Elizabeth Sheridan |
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| description | An EBSL-encoding plasmid, pESBL-PH, was identified during a nosocomial outbreak of <i>Klebsiella pneumoniae</i> ST628 at a United Kingdom general district hospital in 2018. The plasmid from the earliest 2018 <i>K. pneumoniae</i> strain discovered during the outbreak was assembled using both Oxford nanopore long reads and illumina short reads, yielding a fully closed plasmid, pESBL-PH-2018. pESBL-PH-2018 was queried against the complete NCBI RefSeq Plasmid Database, comprising 93,823 plasmids, which was downloaded on 16 July 2024. To identify structurally similar plasmids, strict thresholds were applied, including a mash similarity ≥0.98. This returned 61 plasmids belonging to 13 unique sequence types (STs) hosts. The plasmids were detected from 13 unique countries, dating from 2012 to 2023. The AMR region of the plasmids varied. Interestingly IS<i>26</i>-mediated tandem amplification of resistance genes, including the ESBL gene <i>bla</i><sub>CTX-M-15</sub> was identified in two independent strains, raising their copy number to three. Furthermore, the genomic background of strains carrying a pESBL-PH-2018-like plasmid were analyzed, revealing truncation of the chromosomal <i>ompK36</i> porin gene and carbapenem resistance gene carriage on accessory plasmids in 17.85% and 26.78% of strains with a complete chromosome available. This analysis reveals the widespread dissemination of an ESBL-encoding plasmid in a background of resistance-encoding strains, requiring active surveillance. |
| format | Article |
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| institution | DOAJ |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-0bb070e18ab244c4858eb5b7673fc89c2025-08-20T03:14:29ZengMDPI AGMicrobiology Research2036-74812025-04-011659010.3390/microbiolres16050090The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United KingdomStephen Mark Edward Fordham0Anna Mantzouratou1Elizabeth Sheridan2Department of Life & Environmental Sciences, Talbot Campus Fern Barrow, Bournemouth University, Poole BH12 5BB, UKDepartment of Life & Environmental Sciences, Talbot Campus Fern Barrow, Bournemouth University, Poole BH12 5BB, UKDepartment of Life & Environmental Sciences, Talbot Campus Fern Barrow, Bournemouth University, Poole BH12 5BB, UKAn EBSL-encoding plasmid, pESBL-PH, was identified during a nosocomial outbreak of <i>Klebsiella pneumoniae</i> ST628 at a United Kingdom general district hospital in 2018. The plasmid from the earliest 2018 <i>K. pneumoniae</i> strain discovered during the outbreak was assembled using both Oxford nanopore long reads and illumina short reads, yielding a fully closed plasmid, pESBL-PH-2018. pESBL-PH-2018 was queried against the complete NCBI RefSeq Plasmid Database, comprising 93,823 plasmids, which was downloaded on 16 July 2024. To identify structurally similar plasmids, strict thresholds were applied, including a mash similarity ≥0.98. This returned 61 plasmids belonging to 13 unique sequence types (STs) hosts. The plasmids were detected from 13 unique countries, dating from 2012 to 2023. The AMR region of the plasmids varied. Interestingly IS<i>26</i>-mediated tandem amplification of resistance genes, including the ESBL gene <i>bla</i><sub>CTX-M-15</sub> was identified in two independent strains, raising their copy number to three. Furthermore, the genomic background of strains carrying a pESBL-PH-2018-like plasmid were analyzed, revealing truncation of the chromosomal <i>ompK36</i> porin gene and carbapenem resistance gene carriage on accessory plasmids in 17.85% and 26.78% of strains with a complete chromosome available. This analysis reveals the widespread dissemination of an ESBL-encoding plasmid in a background of resistance-encoding strains, requiring active surveillance.https://www.mdpi.com/2036-7481/16/5/90<i>Klebsiella pneumoniae</i>plasmidsST628outbreak |
| spellingShingle | Stephen Mark Edward Fordham Anna Mantzouratou Elizabeth Sheridan The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United Kingdom Microbiology Research <i>Klebsiella pneumoniae</i> plasmids ST628 outbreak |
| title | The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United Kingdom |
| title_full | The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United Kingdom |
| title_fullStr | The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United Kingdom |
| title_full_unstemmed | The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United Kingdom |
| title_short | The Presence of an ESBL-Encoding Plasmid Reported During a <i>Klebsiella pneumoniae</i> Nosocomial Outbreak in the United Kingdom |
| title_sort | presence of an esbl encoding plasmid reported during a i klebsiella pneumoniae i nosocomial outbreak in the united kingdom |
| topic | <i>Klebsiella pneumoniae</i> plasmids ST628 outbreak |
| url | https://www.mdpi.com/2036-7481/16/5/90 |
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