Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure
Abstract Background Preimplantation embryos in vivo are exposed to various growth factors in the female reproductive tract that are absent in in vitro embryo culture media. Cell-free fat extract exerts antioxidant, anti-ageing, and ovarian function-promoting effects. However, its effects on embryo q...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Reproductive Biology and Endocrinology |
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| Online Access: | https://doi.org/10.1186/s12958-024-01341-4 |
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| author | Xiaowei Zhou Hanfei Zhu Jingru Duan Lan Xia Shen Zhao Huihui Xu Xian Wu Xiaoling Wang Bufang Xu Yi Cao Aijun Zhang |
| author_facet | Xiaowei Zhou Hanfei Zhu Jingru Duan Lan Xia Shen Zhao Huihui Xu Xian Wu Xiaoling Wang Bufang Xu Yi Cao Aijun Zhang |
| author_sort | Xiaowei Zhou |
| collection | DOAJ |
| description | Abstract Background Preimplantation embryos in vivo are exposed to various growth factors in the female reproductive tract that are absent in in vitro embryo culture media. Cell-free fat extract exerts antioxidant, anti-ageing, and ovarian function-promoting effects. However, its effects on embryo quality are yet to be investigated. Methods We assessed the effect of cell-free fat extract supplementation on embryo culture using a naturally ageing mouse model. We assessed the model’s efficacy in influencing embryo development and pregnancy rates in older women with in vitro fertilization failure. In addition, we performed immunofluorescence staining, multiplex immunoassay, whole-genome amplification and DNA sequencing, time-lapse embryo monitoring, and in vitro experiments. Results Cell-free fat extract-supplemented media has a suitable osmolarity and pH and contains high levels of bioactive growth factors. Cell-free fat extract promoted embryo development and implantation in aged mice, probably by increasing embryo growth rate, inhibiting cell apoptosis, and promoting blastocyst adhesion. Clinical results showed that the cell-free fat extract group had significantly higher rates of the day 3 available and high-quality embryos than the control group, and the rate of usable embryos tended to be higher in the cell-free fat extract group. Furthermore, implantation and clinical pregnancy rates improved in the cell-free fat extract group than in the control group. Conclusions Our study implies that cell-free fat extract supplementation can promote embryo development and clinical outcomes and may serve as a rescue strategy for older women with in vitro fertilization failure. |
| format | Article |
| id | doaj-art-0ba8d51742f4439c82ba3561f93784fa |
| institution | Kabale University |
| issn | 1477-7827 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj-art-0ba8d51742f4439c82ba3561f93784fa2025-01-19T12:43:29ZengBMCReproductive Biology and Endocrinology1477-78272025-01-0123111210.1186/s12958-024-01341-4Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failureXiaowei Zhou0Hanfei Zhu1Jingru Duan2Lan Xia3Shen Zhao4Huihui Xu5Xian Wu6Xiaoling Wang7Bufang Xu8Yi Cao9Aijun Zhang10Department of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, The Minhang Hospital of Fudan UniversityDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineAbstract Background Preimplantation embryos in vivo are exposed to various growth factors in the female reproductive tract that are absent in in vitro embryo culture media. Cell-free fat extract exerts antioxidant, anti-ageing, and ovarian function-promoting effects. However, its effects on embryo quality are yet to be investigated. Methods We assessed the effect of cell-free fat extract supplementation on embryo culture using a naturally ageing mouse model. We assessed the model’s efficacy in influencing embryo development and pregnancy rates in older women with in vitro fertilization failure. In addition, we performed immunofluorescence staining, multiplex immunoassay, whole-genome amplification and DNA sequencing, time-lapse embryo monitoring, and in vitro experiments. Results Cell-free fat extract-supplemented media has a suitable osmolarity and pH and contains high levels of bioactive growth factors. Cell-free fat extract promoted embryo development and implantation in aged mice, probably by increasing embryo growth rate, inhibiting cell apoptosis, and promoting blastocyst adhesion. Clinical results showed that the cell-free fat extract group had significantly higher rates of the day 3 available and high-quality embryos than the control group, and the rate of usable embryos tended to be higher in the cell-free fat extract group. Furthermore, implantation and clinical pregnancy rates improved in the cell-free fat extract group than in the control group. Conclusions Our study implies that cell-free fat extract supplementation can promote embryo development and clinical outcomes and may serve as a rescue strategy for older women with in vitro fertilization failure.https://doi.org/10.1186/s12958-024-01341-4Embryo developmentCulture mediaCell-free fat extractGrowth factorIn vitro fertilizationOlder women |
| spellingShingle | Xiaowei Zhou Hanfei Zhu Jingru Duan Lan Xia Shen Zhao Huihui Xu Xian Wu Xiaoling Wang Bufang Xu Yi Cao Aijun Zhang Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure Reproductive Biology and Endocrinology Embryo development Culture media Cell-free fat extract Growth factor In vitro fertilization Older women |
| title | Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure |
| title_full | Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure |
| title_fullStr | Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure |
| title_full_unstemmed | Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure |
| title_short | Cell-free fat extract improves embryo development and clinical outcomes in older women with previous in-vitro fertilization failure |
| title_sort | cell free fat extract improves embryo development and clinical outcomes in older women with previous in vitro fertilization failure |
| topic | Embryo development Culture media Cell-free fat extract Growth factor In vitro fertilization Older women |
| url | https://doi.org/10.1186/s12958-024-01341-4 |
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