Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea
The marine diatom genus <i>Pseudo-nitzschia</i> comprises cosmopolitan phytoplankton species commonly present in the Adriatic Sea. Species within the genus <i>Pseudo-nitzschia</i> have been of significant concern because they produce domoic acid (DA), which can cause amnesic...
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2025-01-01
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| author | Tina Bonačić Jasna Arapov Ivana Bušelić Ivana Lepen Pleić Blanka Milić Roje Tina Tomašević Mia Bužančić Marija Mladinić Silvia Casabianca Antonella Penna Sanda Skejić Živana Ninčević Gladan |
| author_facet | Tina Bonačić Jasna Arapov Ivana Bušelić Ivana Lepen Pleić Blanka Milić Roje Tina Tomašević Mia Bužančić Marija Mladinić Silvia Casabianca Antonella Penna Sanda Skejić Živana Ninčević Gladan |
| author_sort | Tina Bonačić |
| collection | DOAJ |
| description | The marine diatom genus <i>Pseudo-nitzschia</i> comprises cosmopolitan phytoplankton species commonly present in the Adriatic Sea. Species within the genus <i>Pseudo-nitzschia</i> have been of significant concern because they produce domoic acid (DA), which can cause amnesic shellfish poisoning (ASP). In this study, we identified <i>Pseudo-nitzschia</i> species along the Central and Southeastern Adriatic Sea, where monthly sampling carried out from February 2022 to February 2024 allowed for comprehensive species documentation. <i>Pseudo-nitzschia</i> species cell cultures isolated from the study areas were morphologically and molecularly analysed. Morphological analyses were performed using a scanning electron microscope (FE-SEM/STEM), while molecular analyses were conducted, targeting the ITS1-5.8S-ITS2, LSU, and <i>rbc</i>L regions, to confirm species identity. This integrative approach led to the identification of eight species: <i>Pseudo-nitzschia allochrona</i>, <i>Pseudo-nitzschia calliantha</i>, <i>Pseudo-nitzschia delicatissima</i>, <i>Pseudo-nitzschia fraudulenta</i>, <i>Pseudo-nitzschia mannii</i>, <i>Pseudo-nitzschia multistriata</i>, <i>Pseudo-nitzschia pseudodelicatissima</i>, and <i>Pseudo-nitzschia subfraudulenta</i>. Our findings underscore the value of a combined approach for reliable species identification and contribute to the development of genetic sequence databases that support the advancement of next-generation methods such as metabarcoding. This research emphasises the importance of combined morphological and molecular methods for the differentiation of the cryptic and pseudo-cryptic <i>Pseudo-nitzschia</i> species. |
| format | Article |
| id | doaj-art-0b9ef7ef0df6406d8fbd6f3e6f181276 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Plants |
| spelling | doaj-art-0b9ef7ef0df6406d8fbd6f3e6f1812762025-01-24T13:46:55ZengMDPI AGPlants2223-77472025-01-0114224510.3390/plants14020245Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic SeaTina Bonačić0Jasna Arapov1Ivana Bušelić2Ivana Lepen Pleić3Blanka Milić Roje4Tina Tomašević5Mia Bužančić6Marija Mladinić7Silvia Casabianca8Antonella Penna9Sanda Skejić10Živana Ninčević Gladan11Institute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Ravnice 48, 10000 Zagreb, CroatiaDepartment of Biomolecular Sciences, Campus E. Mattei, University of Urbino, Via Ca’ le Suore 2/4, 61029 Urbino, ItalyDepartment of Biomolecular Sciences, Campus E. Mattei, University of Urbino, Via Ca’ le Suore 2/4, 61029 Urbino, ItalyInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaInstitute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, CroatiaThe marine diatom genus <i>Pseudo-nitzschia</i> comprises cosmopolitan phytoplankton species commonly present in the Adriatic Sea. Species within the genus <i>Pseudo-nitzschia</i> have been of significant concern because they produce domoic acid (DA), which can cause amnesic shellfish poisoning (ASP). In this study, we identified <i>Pseudo-nitzschia</i> species along the Central and Southeastern Adriatic Sea, where monthly sampling carried out from February 2022 to February 2024 allowed for comprehensive species documentation. <i>Pseudo-nitzschia</i> species cell cultures isolated from the study areas were morphologically and molecularly analysed. Morphological analyses were performed using a scanning electron microscope (FE-SEM/STEM), while molecular analyses were conducted, targeting the ITS1-5.8S-ITS2, LSU, and <i>rbc</i>L regions, to confirm species identity. This integrative approach led to the identification of eight species: <i>Pseudo-nitzschia allochrona</i>, <i>Pseudo-nitzschia calliantha</i>, <i>Pseudo-nitzschia delicatissima</i>, <i>Pseudo-nitzschia fraudulenta</i>, <i>Pseudo-nitzschia mannii</i>, <i>Pseudo-nitzschia multistriata</i>, <i>Pseudo-nitzschia pseudodelicatissima</i>, and <i>Pseudo-nitzschia subfraudulenta</i>. Our findings underscore the value of a combined approach for reliable species identification and contribute to the development of genetic sequence databases that support the advancement of next-generation methods such as metabarcoding. This research emphasises the importance of combined morphological and molecular methods for the differentiation of the cryptic and pseudo-cryptic <i>Pseudo-nitzschia</i> species.https://www.mdpi.com/2223-7747/14/2/245diatomsmolecular analysismorphologyITS1-5.8S-ITS2LSU<i>rbc</i>L |
| spellingShingle | Tina Bonačić Jasna Arapov Ivana Bušelić Ivana Lepen Pleić Blanka Milić Roje Tina Tomašević Mia Bužančić Marija Mladinić Silvia Casabianca Antonella Penna Sanda Skejić Živana Ninčević Gladan Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea Plants diatoms molecular analysis morphology ITS1-5.8S-ITS2 LSU <i>rbc</i>L |
| title | Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea |
| title_full | Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea |
| title_fullStr | Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea |
| title_full_unstemmed | Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea |
| title_short | Advancing the Taxonomy of the Diatom <i>Pseudo-nitzschia</i> Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea |
| title_sort | advancing the taxonomy of the diatom i pseudo nitzschia i through an integrative study conducted in the central and southeastern adriatic sea |
| topic | diatoms molecular analysis morphology ITS1-5.8S-ITS2 LSU <i>rbc</i>L |
| url | https://www.mdpi.com/2223-7747/14/2/245 |
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