Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks
Box-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0–7.0...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/2879406 |
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| author | Bürge Aşçı Şule Dinç Zor Özlem Aksu Dönmez |
| author_facet | Bürge Aşçı Şule Dinç Zor Özlem Aksu Dönmez |
| author_sort | Bürge Aşçı |
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| description | Box-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0–7.0), flow rate (1.0–1.4 mL/min), and mobile phase ratio (85–95% acetate buffer). Resolution values of all peak pairs were used as a response. Stationary phase was Inertsil OctaDecylSilane- (ODS-) 3V reverse phase column (250 × 4.6 mm, 5 μm) dimensions. The detection was performed at 230 nm. Optimal values were found 6.0 pH, 1.0 mL/min flow rate, and 95% mobile phase ratio for the method which was validated by calculating the linearity (r2>0.9962), accuracy (recoveries ≥ 95.75%), precision (intraday variation ≤ 1.923%, interday variation ≤ 1.950%), limits of detection (LODs), and limits of quantification (LOQs) parameters. LODs and LOQs for analytes were in the range of 0.10–0.19 μg/mL and 0.33–0.63 μg/mL, respectively. The proposed method was applied successfully for the simultaneous determination of the mixtures of five food additives and caffeine in soft drinks. |
| format | Article |
| id | doaj-art-0b9d17cd3c154ea29fa8b2b383b009c6 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-0b9d17cd3c154ea29fa8b2b383b009c62025-02-03T06:11:31ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792016-01-01201610.1155/2016/28794062879406Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft DrinksBürge Aşçı0Şule Dinç Zor1Özlem Aksu Dönmez2Department of Chemistry, Faculty of Science and Arts, Yildiz Technical University, Davutpasa, 34220 Istanbul, TurkeyDepartment of Chemistry, Faculty of Science and Arts, Yildiz Technical University, Davutpasa, 34220 Istanbul, TurkeyDepartment of Chemistry, Faculty of Science and Arts, Yildiz Technical University, Davutpasa, 34220 Istanbul, TurkeyBox-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0–7.0), flow rate (1.0–1.4 mL/min), and mobile phase ratio (85–95% acetate buffer). Resolution values of all peak pairs were used as a response. Stationary phase was Inertsil OctaDecylSilane- (ODS-) 3V reverse phase column (250 × 4.6 mm, 5 μm) dimensions. The detection was performed at 230 nm. Optimal values were found 6.0 pH, 1.0 mL/min flow rate, and 95% mobile phase ratio for the method which was validated by calculating the linearity (r2>0.9962), accuracy (recoveries ≥ 95.75%), precision (intraday variation ≤ 1.923%, interday variation ≤ 1.950%), limits of detection (LODs), and limits of quantification (LOQs) parameters. LODs and LOQs for analytes were in the range of 0.10–0.19 μg/mL and 0.33–0.63 μg/mL, respectively. The proposed method was applied successfully for the simultaneous determination of the mixtures of five food additives and caffeine in soft drinks.http://dx.doi.org/10.1155/2016/2879406 |
| spellingShingle | Bürge Aşçı Şule Dinç Zor Özlem Aksu Dönmez Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks International Journal of Analytical Chemistry |
| title | Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks |
| title_full | Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks |
| title_fullStr | Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks |
| title_full_unstemmed | Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks |
| title_short | Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks |
| title_sort | development and validation of hplc method for the simultaneous determination of five food additives and caffeine in soft drinks |
| url | http://dx.doi.org/10.1155/2016/2879406 |
| work_keys_str_mv | AT burgeascı developmentandvalidationofhplcmethodforthesimultaneousdeterminationoffivefoodadditivesandcaffeineinsoftdrinks AT suledinczor developmentandvalidationofhplcmethodforthesimultaneousdeterminationoffivefoodadditivesandcaffeineinsoftdrinks AT ozlemaksudonmez developmentandvalidationofhplcmethodforthesimultaneousdeterminationoffivefoodadditivesandcaffeineinsoftdrinks |