Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thaliana

Plant growth and development are tightly regulated by cell division, elongation, and differentiation. A visible plant phenotype at the tissue or organ level is coordinated at the cellular level. Among these cellular regulations (cell division, elongation and differentiation), cell division in plants...

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Main Authors: Olivia S. Hazelwood, M. Arif Ashraf
Format: Article
Language:English
Published: Cambridge University Press 2024-01-01
Series:Quantitative Plant Biology
Subjects:
Online Access:https://www.cambridge.org/core/product/identifier/S2632882824000183/type/journal_article
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author Olivia S. Hazelwood
M. Arif Ashraf
author_facet Olivia S. Hazelwood
M. Arif Ashraf
author_sort Olivia S. Hazelwood
collection DOAJ
description Plant growth and development are tightly regulated by cell division, elongation, and differentiation. A visible plant phenotype at the tissue or organ level is coordinated at the cellular level. Among these cellular regulations (cell division, elongation and differentiation), cell division in plants follows the same universal mechanisms across kingdoms of life, and involves conserved cell cycle regulatory proteins (cyclins, cyclin-dependent kinase and cell cycle inhibitors). Cell division is regulated through distinct cell cycle steps (G1, S, G2 and M), and these individual steps are visualised using transgenic marker lines. As a result, a quantitative cell cycle approach in plants during development and stress conditions relies on the accuracy of cell cycle markers. In this perspective article, we highlight the available cell cycle marker lines in plants, common practices within plant biology communities based on existing literature and provide a road map to a thorough quantitative approach of cell cycle regulation in plants.
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publisher Cambridge University Press
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series Quantitative Plant Biology
spelling doaj-art-0b986731be5840b2b3ba286aad7042fb2025-08-20T02:45:15ZengCambridge University PressQuantitative Plant Biology2632-88282024-01-01510.1017/qpb.2024.18Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thalianaOlivia S. Hazelwood0M. Arif Ashraf1https://orcid.org/0000-0002-0879-5835Department of Biology, Howard University, Washington, DC, USADepartment of Biology, Howard University, Washington, DC, USAPlant growth and development are tightly regulated by cell division, elongation, and differentiation. A visible plant phenotype at the tissue or organ level is coordinated at the cellular level. Among these cellular regulations (cell division, elongation and differentiation), cell division in plants follows the same universal mechanisms across kingdoms of life, and involves conserved cell cycle regulatory proteins (cyclins, cyclin-dependent kinase and cell cycle inhibitors). Cell division is regulated through distinct cell cycle steps (G1, S, G2 and M), and these individual steps are visualised using transgenic marker lines. As a result, a quantitative cell cycle approach in plants during development and stress conditions relies on the accuracy of cell cycle markers. In this perspective article, we highlight the available cell cycle marker lines in plants, common practices within plant biology communities based on existing literature and provide a road map to a thorough quantitative approach of cell cycle regulation in plants.https://www.cambridge.org/core/product/identifier/S2632882824000183/type/journal_articleabiotic stresscell biologycell cycleplant developmentquantitative cell biology
spellingShingle Olivia S. Hazelwood
M. Arif Ashraf
Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thaliana
Quantitative Plant Biology
abiotic stress
cell biology
cell cycle
plant development
quantitative cell biology
title Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thaliana
title_full Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thaliana
title_fullStr Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thaliana
title_full_unstemmed Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thaliana
title_short Molecular markers in cell cycle visualisation during development and stress conditions in Arabidopsis thaliana
title_sort molecular markers in cell cycle visualisation during development and stress conditions in arabidopsis thaliana
topic abiotic stress
cell biology
cell cycle
plant development
quantitative cell biology
url https://www.cambridge.org/core/product/identifier/S2632882824000183/type/journal_article
work_keys_str_mv AT oliviashazelwood molecularmarkersincellcyclevisualisationduringdevelopmentandstressconditionsinarabidopsisthaliana
AT marifashraf molecularmarkersincellcyclevisualisationduringdevelopmentandstressconditionsinarabidopsisthaliana