Estimation of Lamotrigine by RP-HPLC Method

A rapid and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of lamotrigine in its pure form as well as in pharmaceutical dosage forms. Chromatography was carried out on a Luna C18 column using a mixture of potassium dihydrogen phosphate...

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Main Authors: D. Anantha Kumar, CH. Venkata Kumar, P. Seetharamaiah, J. V. L. N. Seshagiri Rao
Format: Article
Language:English
Published: Wiley 2010-01-01
Series:E-Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2010/754745
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author D. Anantha Kumar
CH. Venkata Kumar
P. Seetharamaiah
J. V. L. N. Seshagiri Rao
author_facet D. Anantha Kumar
CH. Venkata Kumar
P. Seetharamaiah
J. V. L. N. Seshagiri Rao
author_sort D. Anantha Kumar
collection DOAJ
description A rapid and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of lamotrigine in its pure form as well as in pharmaceutical dosage forms. Chromatography was carried out on a Luna C18 column using a mixture of potassium dihydrogen phosphate buffer (pH 7.3) and methanol in a ratio of 60:40 v/v as the mobile phase at a flow rate of 1.0 mL/min. The detection was done at 305 nm. The retention time of the drug was 6.1 min. The method produced linear responses in the concentration range of 10 to 70 μg/mL of lamotrigine. The method was found to be reproducible for analysis of the drug in tablet dosage forms.
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2090-9810
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publishDate 2010-01-01
publisher Wiley
record_format Article
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spelling doaj-art-0b98016ca6514e22a4090b2972bf08732025-08-20T02:03:28ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017S1S203S20810.1155/2010/754745Estimation of Lamotrigine by RP-HPLC MethodD. Anantha Kumar0CH. Venkata Kumar1P. Seetharamaiah2J. V. L. N. Seshagiri Rao3Pharmaceutical Analysis and Quality Assurance Division, College of Pharmaceutical Sciences Andhra University, Visakhapatnam-530 003, IndiaPharmaceutical Analysis and Quality Assurance Division, College of Pharmaceutical Sciences Andhra University, Visakhapatnam-530 003, IndiaPharmaceutical Analysis and Quality Assurance Division, College of Pharmaceutical Sciences Andhra University, Visakhapatnam-530 003, IndiaPharmaceutical Analysis and Quality Assurance Division, College of Pharmaceutical Sciences Andhra University, Visakhapatnam-530 003, IndiaA rapid and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of lamotrigine in its pure form as well as in pharmaceutical dosage forms. Chromatography was carried out on a Luna C18 column using a mixture of potassium dihydrogen phosphate buffer (pH 7.3) and methanol in a ratio of 60:40 v/v as the mobile phase at a flow rate of 1.0 mL/min. The detection was done at 305 nm. The retention time of the drug was 6.1 min. The method produced linear responses in the concentration range of 10 to 70 μg/mL of lamotrigine. The method was found to be reproducible for analysis of the drug in tablet dosage forms.http://dx.doi.org/10.1155/2010/754745
spellingShingle D. Anantha Kumar
CH. Venkata Kumar
P. Seetharamaiah
J. V. L. N. Seshagiri Rao
Estimation of Lamotrigine by RP-HPLC Method
E-Journal of Chemistry
title Estimation of Lamotrigine by RP-HPLC Method
title_full Estimation of Lamotrigine by RP-HPLC Method
title_fullStr Estimation of Lamotrigine by RP-HPLC Method
title_full_unstemmed Estimation of Lamotrigine by RP-HPLC Method
title_short Estimation of Lamotrigine by RP-HPLC Method
title_sort estimation of lamotrigine by rp hplc method
url http://dx.doi.org/10.1155/2010/754745
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