Pre‐clinical enteropathy in healthy soft‐coated wheaten terriers

Abstract Background Soft‐coated wheaten terriers (SCWTs) have a predisposition to the development of protein‐losing enteropathy (PLE). Early recognition of disease may improve morbidity and mortality in these and other at‐risk dogs. Preclinical inflammatory bowel disease (IBD), characterized by incr...

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Main Authors: M. Katherine Tolbert, Jewels Darrow, Louise Grubb, Stuart Fitzgerald, Rebecca Bergee, Josh Price, Marcos Mariano, Michael Hong, Chi‐Hsuan Sung, Tracy Hill, Joerg M. Steiner, Jan S. Suchodolski
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Journal of Veterinary Internal Medicine
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Online Access:https://doi.org/10.1111/jvim.17293
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author M. Katherine Tolbert
Jewels Darrow
Louise Grubb
Stuart Fitzgerald
Rebecca Bergee
Josh Price
Marcos Mariano
Michael Hong
Chi‐Hsuan Sung
Tracy Hill
Joerg M. Steiner
Jan S. Suchodolski
author_facet M. Katherine Tolbert
Jewels Darrow
Louise Grubb
Stuart Fitzgerald
Rebecca Bergee
Josh Price
Marcos Mariano
Michael Hong
Chi‐Hsuan Sung
Tracy Hill
Joerg M. Steiner
Jan S. Suchodolski
author_sort M. Katherine Tolbert
collection DOAJ
description Abstract Background Soft‐coated wheaten terriers (SCWTs) have a predisposition to the development of protein‐losing enteropathy (PLE). Early recognition of disease may improve morbidity and mortality in these and other at‐risk dogs. Preclinical inflammatory bowel disease (IBD), characterized by increased intestinal permeability, immune dysregulation and inflammation, and changes to the gut microbial composition, or biochemical evidence of disease many years before development of clinical signs, has been proposed for people at risk for IBD. Hypothesis/Objectives Determine if changes in fecal metabolites and intestinal permeability could be identified in SCWTs before development of clinical signs. We hypothesized that, in contrast to healthy non‐SCWT dogs, healthy SCWT would have changes similar to those of dogs with PLE. Animals Twelve healthy SCWTs, 10 healthy non‐SCWTs, and 8 PLE dogs. Methods Prospective study. Fecal calprotectin, targeted metabolites and unconjugated bile acids, intestinal permeability testing, and video capsule endoscopy were evaluated. Single‐factor analysis of variance (ANOVA) was performed to evaluate fecal metabolites and bile acids for group differences. A repeated‐measures mixed model ANOVA was performed for blood lactulose:galactose area under the curve (AUC). Results Significant differences among groups were found for several fecal fatty acids and sterols. Healthy non‐SCWT dogs, but not healthy SCWTs, were found to have significantly lower AUCs than PLE dogs (P = .04). Conclusions Healthy SCWT dogs had changes in several biomarkers used to identify preclinical IBD in humans.
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spelling doaj-art-0b97e0818db9425cb91c67fffe0f2c1d2025-08-20T02:14:23ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-03-01392n/an/a10.1111/jvim.17293Pre‐clinical enteropathy in healthy soft‐coated wheaten terriersM. Katherine Tolbert0Jewels Darrow1Louise Grubb2Stuart Fitzgerald3Rebecca Bergee4Josh Price5Marcos Mariano6Michael Hong7Chi‐Hsuan Sung8Tracy Hill9Joerg M. Steiner10Jan S. Suchodolski11Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station Texas USACollege of Veterinary Medicine North Carolina State University Raleigh North Carolina USATrivium Vet Waterford IrelandTrivium Vet Waterford IrelandOIT Research Consulting Support University of Tennessee Knoxville Tennessee USAOIT Research Consulting Support University of Tennessee Knoxville Tennessee USAPharmaceutical and Molecular Biotechnology Research Centre South East Technological University Waterford IrelandGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station Texas USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station Texas USAEthos Veterinary Health, Inc. Westlake Village California USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station Texas USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station Texas USAAbstract Background Soft‐coated wheaten terriers (SCWTs) have a predisposition to the development of protein‐losing enteropathy (PLE). Early recognition of disease may improve morbidity and mortality in these and other at‐risk dogs. Preclinical inflammatory bowel disease (IBD), characterized by increased intestinal permeability, immune dysregulation and inflammation, and changes to the gut microbial composition, or biochemical evidence of disease many years before development of clinical signs, has been proposed for people at risk for IBD. Hypothesis/Objectives Determine if changes in fecal metabolites and intestinal permeability could be identified in SCWTs before development of clinical signs. We hypothesized that, in contrast to healthy non‐SCWT dogs, healthy SCWT would have changes similar to those of dogs with PLE. Animals Twelve healthy SCWTs, 10 healthy non‐SCWTs, and 8 PLE dogs. Methods Prospective study. Fecal calprotectin, targeted metabolites and unconjugated bile acids, intestinal permeability testing, and video capsule endoscopy were evaluated. Single‐factor analysis of variance (ANOVA) was performed to evaluate fecal metabolites and bile acids for group differences. A repeated‐measures mixed model ANOVA was performed for blood lactulose:galactose area under the curve (AUC). Results Significant differences among groups were found for several fecal fatty acids and sterols. Healthy non‐SCWT dogs, but not healthy SCWTs, were found to have significantly lower AUCs than PLE dogs (P = .04). Conclusions Healthy SCWT dogs had changes in several biomarkers used to identify preclinical IBD in humans.https://doi.org/10.1111/jvim.17293calprotectincaninedysbiosisintestinal permeabilityleaky gutprotein‐losing enteropathy
spellingShingle M. Katherine Tolbert
Jewels Darrow
Louise Grubb
Stuart Fitzgerald
Rebecca Bergee
Josh Price
Marcos Mariano
Michael Hong
Chi‐Hsuan Sung
Tracy Hill
Joerg M. Steiner
Jan S. Suchodolski
Pre‐clinical enteropathy in healthy soft‐coated wheaten terriers
Journal of Veterinary Internal Medicine
calprotectin
canine
dysbiosis
intestinal permeability
leaky gut
protein‐losing enteropathy
title Pre‐clinical enteropathy in healthy soft‐coated wheaten terriers
title_full Pre‐clinical enteropathy in healthy soft‐coated wheaten terriers
title_fullStr Pre‐clinical enteropathy in healthy soft‐coated wheaten terriers
title_full_unstemmed Pre‐clinical enteropathy in healthy soft‐coated wheaten terriers
title_short Pre‐clinical enteropathy in healthy soft‐coated wheaten terriers
title_sort pre clinical enteropathy in healthy soft coated wheaten terriers
topic calprotectin
canine
dysbiosis
intestinal permeability
leaky gut
protein‐losing enteropathy
url https://doi.org/10.1111/jvim.17293
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