A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantel
This study was designed to compare the effectiveness of ivermectin (IVM) with praziquantel (PZQ) in treating Schistosoma mansoni-infected mice through biological and proteomic analysis. Detecting protein structure changes in the worms after treatment could help pursue drug efficacy in schistosomiasi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1646155/full |
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| author | Eman Sayed El-Wakil Mona Mohamed Tolba Mona Hasan El-Sayad Maha Khairy Hassen Hayat S. Al-Rashidi Mina A. Almayouf Wafa Abdullah I. Al-Megrin Hind Alzaylaee Esraa Abdelhamid Moneer |
| author_facet | Eman Sayed El-Wakil Mona Mohamed Tolba Mona Hasan El-Sayad Maha Khairy Hassen Hayat S. Al-Rashidi Mina A. Almayouf Wafa Abdullah I. Al-Megrin Hind Alzaylaee Esraa Abdelhamid Moneer |
| author_sort | Eman Sayed El-Wakil |
| collection | DOAJ |
| description | This study was designed to compare the effectiveness of ivermectin (IVM) with praziquantel (PZQ) in treating Schistosoma mansoni-infected mice through biological and proteomic analysis. Detecting protein structure changes in the worms after treatment could help pursue drug efficacy in schistosomiasis. Sixty Swiss albino mice were infected with S. mansoni cercariae and were divided into three major groups (Infected untreated control, praziquantel-treated, and ivermectin-treated). The evaluation of treatment was performed by parasitological, histopathological analysis, scanning electron microscopy (SEM), and proteomic analysis of the worms through lysis and protein extraction, SDS-PAGE, Mass Spectrometry, and data analysis. The treated groups significantly reduced mean worm load and ova count with smaller granulomas compared to the infected control group. In adult worms treated with PZQ and IVM, severe tegumental destruction, peeling, erosion, ulceration, and suckers damage were detected by SEM. The proteomic study identified 19 protein bands, 12 commonly shared proteins between all studied groups, and seven differential protein bands. Molecular and biological function administered from the NCBInr database revealed the presence of glycolytic proteins, structural proteins, and cytosol stress response. Although praziquantel outperformed ivermectin, the anti-schistosomal properties of ivermectin are encouraging, evidenced by changes in the protein structure of the worms detected after ivermectin treatment. This may open the way to use ivermectin in combination with other anti-schistosomal medicines to avoid any potential resistance from monotherapy. Besides, it highlights the role of proteomic analysis in differential protein identification that could help efficiently treat schistosomiasis. |
| format | Article |
| id | doaj-art-0b850adcea6f421db4f1d30b52abbb64 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-0b850adcea6f421db4f1d30b52abbb642025-08-20T03:31:30ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-07-011210.3389/fvets.2025.16461551646155A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantelEman Sayed El-Wakil0Mona Mohamed Tolba1Mona Hasan El-Sayad2Maha Khairy Hassen3Hayat S. Al-Rashidi4Mina A. Almayouf5Wafa Abdullah I. Al-Megrin6Hind Alzaylaee7Esraa Abdelhamid Moneer8Department of Parasitology, Theodor Bilharz Research Institute, Kornaish El-Nile, Giza, EgyptParasitology Department, Medical Research Institute, Alexandria University, Alexandria, EgyptParasitology Department, Medical Research Institute, Alexandria University, Alexandria, EgyptDepartment of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, EgyptDepartment of Biology, College of Science, Qassim University, Buraydah, Saudi ArabiaDepartment of Biology, College of Science, Qassim University, Buraydah, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, EgyptThis study was designed to compare the effectiveness of ivermectin (IVM) with praziquantel (PZQ) in treating Schistosoma mansoni-infected mice through biological and proteomic analysis. Detecting protein structure changes in the worms after treatment could help pursue drug efficacy in schistosomiasis. Sixty Swiss albino mice were infected with S. mansoni cercariae and were divided into three major groups (Infected untreated control, praziquantel-treated, and ivermectin-treated). The evaluation of treatment was performed by parasitological, histopathological analysis, scanning electron microscopy (SEM), and proteomic analysis of the worms through lysis and protein extraction, SDS-PAGE, Mass Spectrometry, and data analysis. The treated groups significantly reduced mean worm load and ova count with smaller granulomas compared to the infected control group. In adult worms treated with PZQ and IVM, severe tegumental destruction, peeling, erosion, ulceration, and suckers damage were detected by SEM. The proteomic study identified 19 protein bands, 12 commonly shared proteins between all studied groups, and seven differential protein bands. Molecular and biological function administered from the NCBInr database revealed the presence of glycolytic proteins, structural proteins, and cytosol stress response. Although praziquantel outperformed ivermectin, the anti-schistosomal properties of ivermectin are encouraging, evidenced by changes in the protein structure of the worms detected after ivermectin treatment. This may open the way to use ivermectin in combination with other anti-schistosomal medicines to avoid any potential resistance from monotherapy. Besides, it highlights the role of proteomic analysis in differential protein identification that could help efficiently treat schistosomiasis.https://www.frontiersin.org/articles/10.3389/fvets.2025.1646155/fullivermectinpraziquantelproteomic analysisscanning electron microscopeSchistosoma mansoni |
| spellingShingle | Eman Sayed El-Wakil Mona Mohamed Tolba Mona Hasan El-Sayad Maha Khairy Hassen Hayat S. Al-Rashidi Mina A. Almayouf Wafa Abdullah I. Al-Megrin Hind Alzaylaee Esraa Abdelhamid Moneer A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantel Frontiers in Veterinary Science ivermectin praziquantel proteomic analysis scanning electron microscope Schistosoma mansoni |
| title | A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantel |
| title_full | A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantel |
| title_fullStr | A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantel |
| title_full_unstemmed | A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantel |
| title_short | A comparative parasitological, histopathological, and proteomic analysis of Schistosoma mansoni infected mice treated with ivermectin and praziquantel |
| title_sort | comparative parasitological histopathological and proteomic analysis of schistosoma mansoni infected mice treated with ivermectin and praziquantel |
| topic | ivermectin praziquantel proteomic analysis scanning electron microscope Schistosoma mansoni |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1646155/full |
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