Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphere
Abstract This study investigated plant growth-promoting (PGP) mechanisms in Priestia aryabhattai VMYP6 and Paenibacillus sp. VMY10, isolated from tomato roots. Their genomes were initially assessed in silico through various approaches, and these observations were then compared with results obtained...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-87390-0 |
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| author | Carolina Almirón Tomás Denis Petitti María Agustina Ponso Ana María Romero Vanessa Andrea Areco María Isabel Bianco Martín Espariz Pablo Marcelo Yaryura |
| author_facet | Carolina Almirón Tomás Denis Petitti María Agustina Ponso Ana María Romero Vanessa Andrea Areco María Isabel Bianco Martín Espariz Pablo Marcelo Yaryura |
| author_sort | Carolina Almirón |
| collection | DOAJ |
| description | Abstract This study investigated plant growth-promoting (PGP) mechanisms in Priestia aryabhattai VMYP6 and Paenibacillus sp. VMY10, isolated from tomato roots. Their genomes were initially assessed in silico through various approaches, and these observations were then compared with results obtained in vitro and in vivo. Both possess genes associated with the production of siderophores, indole acetic acid (IAA) and cytokinins (CKs), all of which have been shown to promote plant growth. The two strains were able to produce these compounds in vitro. Although both genomes harbor genes for phosphorus solubilization, only VMY10 demonstrated this ability in vitro. Genes linked to flagellar assembly and chemotaxis were identified in the two cases. Both strains were able to colonize plant roots, even though VMYP6 lacked motility and no flagella were observed microscopically. In the greenhouse, tomato plants inoculated with the strains showed increased biomass, leaf area, and root length. These findings underscore the importance of integrating in vitro assays, genomic analyses, and plant trials to gain a comprehensive insight into the PGP mechanisms of rhizobacteria like VMYP6 and VMY10. Such insight may contribute to improving the selection of strains used as biofertilizers in tomato, a major crop worldwide. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-0b80385e2f044a749a3ea59f81ead21d2025-02-02T12:21:16ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-87390-0Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphereCarolina Almirón0Tomás Denis Petitti1María Agustina Ponso2Ana María Romero3Vanessa Andrea Areco4María Isabel Bianco5Martín Espariz6Pablo Marcelo Yaryura7Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB, UNVM-CONICET)Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICETInstituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB, UNVM-CONICET)Cátedra de Fitopatología, Departamento de Producción Vegetal, Facultad de Agronomía, Universidad de Buenos AiresInstituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB, UNVM-CONICET)Instituto de Ciencia y Tecnología Dr. César Milstein – Fundación Pablo Cassará – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICETInstituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB, UNVM-CONICET)Abstract This study investigated plant growth-promoting (PGP) mechanisms in Priestia aryabhattai VMYP6 and Paenibacillus sp. VMY10, isolated from tomato roots. Their genomes were initially assessed in silico through various approaches, and these observations were then compared with results obtained in vitro and in vivo. Both possess genes associated with the production of siderophores, indole acetic acid (IAA) and cytokinins (CKs), all of which have been shown to promote plant growth. The two strains were able to produce these compounds in vitro. Although both genomes harbor genes for phosphorus solubilization, only VMY10 demonstrated this ability in vitro. Genes linked to flagellar assembly and chemotaxis were identified in the two cases. Both strains were able to colonize plant roots, even though VMYP6 lacked motility and no flagella were observed microscopically. In the greenhouse, tomato plants inoculated with the strains showed increased biomass, leaf area, and root length. These findings underscore the importance of integrating in vitro assays, genomic analyses, and plant trials to gain a comprehensive insight into the PGP mechanisms of rhizobacteria like VMYP6 and VMY10. Such insight may contribute to improving the selection of strains used as biofertilizers in tomato, a major crop worldwide.https://doi.org/10.1038/s41598-025-87390-0 |
| spellingShingle | Carolina Almirón Tomás Denis Petitti María Agustina Ponso Ana María Romero Vanessa Andrea Areco María Isabel Bianco Martín Espariz Pablo Marcelo Yaryura Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphere Scientific Reports |
| title | Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphere |
| title_full | Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphere |
| title_fullStr | Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphere |
| title_full_unstemmed | Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphere |
| title_short | Functional and genomic analyses of plant growth promoting traits in Priestia aryabhattai and Paenibacillus sp. isolates from tomato rhizosphere |
| title_sort | functional and genomic analyses of plant growth promoting traits in priestia aryabhattai and paenibacillus sp isolates from tomato rhizosphere |
| url | https://doi.org/10.1038/s41598-025-87390-0 |
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