The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats
The aim of the research was to determine possible changes in the morphology of cells of the leukocyte order of peripheral blood, using geometric morphometric tests, after the application of calcium-aluminate and calcium-silicate cements to the dental pulp in rats. The study included 27 Wistar rats,...
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Kafkas University, Faculty of Veterinary Medicine
2024-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3157 |
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| author | Muhamed KATICA Zurifa AJANOVIĆ Aida BEŠIĆ Ognjenka JANKOVIĆ Emina DERVIŠEVIĆ Nedžad HADŽIOMEROVIĆ Faruk TANDIR 5 Smiljana PARAŠ Amina LUČKIN Vukoman JOKANOVIĆ |
| author_facet | Muhamed KATICA Zurifa AJANOVIĆ Aida BEŠIĆ Ognjenka JANKOVIĆ Emina DERVIŠEVIĆ Nedžad HADŽIOMEROVIĆ Faruk TANDIR 5 Smiljana PARAŠ Amina LUČKIN Vukoman JOKANOVIĆ |
| author_sort | Muhamed KATICA |
| collection | DOAJ |
| description | The aim of the research was to determine possible changes in the morphology of cells
of the leukocyte order of peripheral blood, using geometric morphometric tests, after
the application of calcium-aluminate and calcium-silicate cements to the dental pulp
in rats. The study included 27 Wistar rats, divided into an experimental group (n=18)
and a control group (n=9). Trepanation of the tip of the pulp cavity was performed,
and placement of calcium-aluminate and calcium-silicate dental cements directly on
the pulp. Peripheral blood samples were collected by vena caudalis puncture, with the
aim of making blood smears. In the tpsUtil program, two-dimensional models of the
examined leukocytes were created and they were converted into tps files, on which
sixteen specific points were marked in the tpsDig program. We analyzed their shape
in the MorphoJ program. The results of discriminant functional analysis determined
that there was a statistically significant difference in the shape of the lymphocytes
between the experimental animals, to which dental cements were applied, compared to
the lymphocytes from the control group. Morphological differences were determined
between the lymphocytes to which calcium aluminate and calcium silicate were applied.
The results indicate that there were statistically significant morphological differences
between these two groups of lymphocytes (P=0.02). The results obtained indicate the
possibly unfavorable influence of the tested dental cements, especially calcium silicate,
on leukocytic cells. |
| format | Article |
| id | doaj-art-0b7f3661beb04209a100c269666e1995 |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-0b7f3661beb04209a100c269666e19952025-01-04T21:15:10ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512024-11-0131111111710.9775/kvfd.2024.329473157The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar ratsMuhamed KATICA0Zurifa AJANOVIĆ1Aida BEŠIĆ2Ognjenka JANKOVIĆ3Emina DERVIŠEVIĆ4Nedžad HADŽIOMEROVIĆ5Faruk TANDIR 5 Smiljana PARAŠ6Amina LUČKIN7Vukoman JOKANOVIĆ8University of Sarajevo, Veterinary Faculty, Department of Clinical Sciences of Veterinary Medicine, 71000 Sarajevo, BOSNIA AND HERZEGOVINAUniversity of Sarajevo, Faculty of Medicine, Department of Anatomy, 71000 Sarajevo, BOSNIA AND HERZEGOVINA 3 University of Banja Luka, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, 78000 Banja Luka, BOSNIA AND HERZEGOVINAUniversity of Sarajevo, Veterinary Faculty, Department of Clinical Sciences of Veterinary Medicine, 71000 Sarajevo, BOSNIA AND HERZEGOVINAUniversity of Sarajevo, Faculty of Medicine, Department of Forensic Medicine, 71000 Sarajevo, BOSNIA AND HERZEGOVINAUniversity of Sarajevo, Veterinary Faculty, Department of Basic Sciences of Veterinary Medicine, 71000 Sarajevo, BOSNIA AND HERZEGOVINAUniversity of Banja Luka, Faculty of Science and Mathematics, Department of Zoology, 78 000 Banja Luka, BOSNIA AND HERZEGOVINA7 University of Sarajevo, Clinic for Orthopedics and Traumatology, Clinical Center University of Sarajevo, 71 000 Sarajevo, BOSNIA AND HERZEGOVINAInovative Company ALBOS doo, 11 000 Belgrade, SERBIAUniversity of Belgrade, Vinča Institute of Nuclear Sciences, 11 000 Belgrade, SERBIAThe aim of the research was to determine possible changes in the morphology of cells of the leukocyte order of peripheral blood, using geometric morphometric tests, after the application of calcium-aluminate and calcium-silicate cements to the dental pulp in rats. The study included 27 Wistar rats, divided into an experimental group (n=18) and a control group (n=9). Trepanation of the tip of the pulp cavity was performed, and placement of calcium-aluminate and calcium-silicate dental cements directly on the pulp. Peripheral blood samples were collected by vena caudalis puncture, with the aim of making blood smears. In the tpsUtil program, two-dimensional models of the examined leukocytes were created and they were converted into tps files, on which sixteen specific points were marked in the tpsDig program. We analyzed their shape in the MorphoJ program. The results of discriminant functional analysis determined that there was a statistically significant difference in the shape of the lymphocytes between the experimental animals, to which dental cements were applied, compared to the lymphocytes from the control group. Morphological differences were determined between the lymphocytes to which calcium aluminate and calcium silicate were applied. The results indicate that there were statistically significant morphological differences between these two groups of lymphocytes (P=0.02). The results obtained indicate the possibly unfavorable influence of the tested dental cements, especially calcium silicate, on leukocytic cells.https://vetdergikafkas.org/pdf.php?id=3157biomaterialsleukocyte cellsdentinrat |
| spellingShingle | Muhamed KATICA Zurifa AJANOVIĆ Aida BEŠIĆ Ognjenka JANKOVIĆ Emina DERVIŠEVIĆ Nedžad HADŽIOMEROVIĆ Faruk TANDIR 5 Smiljana PARAŠ Amina LUČKIN Vukoman JOKANOVIĆ The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats Kafkas Universitesi Veteriner Fakültesi Dergisi biomaterials leukocyte cells dentin rat |
| title | The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats |
| title_full | The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats |
| title_fullStr | The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats |
| title_full_unstemmed | The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats |
| title_short | The assessment of white blood corpuscles by geometric-morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats |
| title_sort | assessment of white blood corpuscles by geometric morphometric analysis after the application of calcium aluminate and calcium silicate dental cements in wistar rats |
| topic | biomaterials leukocyte cells dentin rat |
| url | https://vetdergikafkas.org/pdf.php?id=3157 |
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