Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains
Background: The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated different solvent extracts...
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The Journal of Infection in Developing Countries
2010-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/621 |
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| author | Rosina Khan Mohammad Zakir Sadul H Afaq Abdul Latif Asad U Khan |
| author_facet | Rosina Khan Mohammad Zakir Sadul H Afaq Abdul Latif Asad U Khan |
| author_sort | Rosina Khan |
| collection | DOAJ |
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Background: The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Prosopis spicigera (P. Spicigera), Zingiber officinale, and Trachyspermum ammi (T. ammi) to determine their efficacy against multidrug resistant microbes.
Methodology: Successive extractions of these plants were performed using a Soxhlet apparatus, using solvents with increasing polarities. Preliminary phytochemical analysis was also performed .Minimum inhibitory concentration was determined by a two-fold serial dilution method followed by determination of minimum bactericidal/fungicidal concentration. Multidrug resistant (MDR) strains of Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, Escherichia coli and reference strains of Streptococcus mutans and Streptococcus bovis were used in the study.
Results: The ethanolic fraction of P. spicigera (least minimum inhibitory concentration [MIC] - 4.88 µg/ml) demonstrated a remarkable inhibition of the microorganisms while fractions obtained from those of Zingiber officinale (least MIC-78.125 µg/ml) exhibited little activity. The petroleum ether fraction of T. ammi (least MIC- 625 µg/ml) showed best activity when compared to its other fractions. Qualitative analysis of the phytoconstituents was also performed.
Conclusions: The potency shown by these extracts recommends their use against multidrug resistant microorganisms. This study also showed that P. spicigera could be a potential source of new antimicrobial agents.
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| format | Article |
| id | doaj-art-0b7e3ff61ec34df08dbda4428c1e7726 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-05-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-0b7e3ff61ec34df08dbda4428c1e77262025-08-20T02:27:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-05-0140510.3855/jidc.621Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strainsRosina Khan0Mohammad Zakir1Sadul H Afaq2Abdul Latif3Asad U Khan4Aligarh Muslim University, Aligarh, IndiaAligarh Muslim University, Aligarh, IndiaAligarh Muslim University, Aligarh, IndiaAligarh Muslim University, Aligarh, IndiaAligarh Muslim University, Aligarh, India Background: The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Prosopis spicigera (P. Spicigera), Zingiber officinale, and Trachyspermum ammi (T. ammi) to determine their efficacy against multidrug resistant microbes. Methodology: Successive extractions of these plants were performed using a Soxhlet apparatus, using solvents with increasing polarities. Preliminary phytochemical analysis was also performed .Minimum inhibitory concentration was determined by a two-fold serial dilution method followed by determination of minimum bactericidal/fungicidal concentration. Multidrug resistant (MDR) strains of Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, Escherichia coli and reference strains of Streptococcus mutans and Streptococcus bovis were used in the study. Results: The ethanolic fraction of P. spicigera (least minimum inhibitory concentration [MIC] - 4.88 µg/ml) demonstrated a remarkable inhibition of the microorganisms while fractions obtained from those of Zingiber officinale (least MIC-78.125 µg/ml) exhibited little activity. The petroleum ether fraction of T. ammi (least MIC- 625 µg/ml) showed best activity when compared to its other fractions. Qualitative analysis of the phytoconstituents was also performed. Conclusions: The potency shown by these extracts recommends their use against multidrug resistant microorganisms. This study also showed that P. spicigera could be a potential source of new antimicrobial agents. https://jidc.org/index.php/journal/article/view/621Multidrug resistanceESBLsplant extractsbacteriafungus |
| spellingShingle | Rosina Khan Mohammad Zakir Sadul H Afaq Abdul Latif Asad U Khan Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains Journal of Infection in Developing Countries Multidrug resistance ESBLs plant extracts bacteria fungus |
| title | Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains |
| title_full | Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains |
| title_fullStr | Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains |
| title_full_unstemmed | Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains |
| title_short | Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains |
| title_sort | activity of solvent extracts of prosopis spicigera zingiber officinale and trachyspermum ammi against multidrug resistant bacterial and fungal strains |
| topic | Multidrug resistance ESBLs plant extracts bacteria fungus |
| url | https://jidc.org/index.php/journal/article/view/621 |
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