Innovations, challenges, and minimal information for standardization of humanized mice
Abstract Mice xenotransplanted with human cells and/or expressing human gene products (also known as “humanized mice”) recapitulate the human evolutionary specialization and diversity of genotypic and phenotypic traits. These models can provide a relevant in vivo context for understanding of human‐s...
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| Format: | Article |
| Language: | English |
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Springer Nature
2020-06-01
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| Series: | EMBO Molecular Medicine |
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| Online Access: | https://doi.org/10.15252/emmm.201708662 |
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| author | Renata Stripecke Christian Münz Jan Jacob Schuringa Karl‐Dimiter Bissig Brian Soper Terrence Meeham Li‐Chin Yao James P Di Santo Michael Brehm Estefania Rodriguez Anja Kathrin Wege Dominique Bonnet Silvia Guionaud Kristina E Howard Scott Kitchen Florian Klein Kourosh Saeb‐Parsy Johannes Sam Amar Deep Sharma Andreas Trumpp Livio Trusolino Carol Bult Leonard Shultz |
| author_facet | Renata Stripecke Christian Münz Jan Jacob Schuringa Karl‐Dimiter Bissig Brian Soper Terrence Meeham Li‐Chin Yao James P Di Santo Michael Brehm Estefania Rodriguez Anja Kathrin Wege Dominique Bonnet Silvia Guionaud Kristina E Howard Scott Kitchen Florian Klein Kourosh Saeb‐Parsy Johannes Sam Amar Deep Sharma Andreas Trumpp Livio Trusolino Carol Bult Leonard Shultz |
| author_sort | Renata Stripecke |
| collection | DOAJ |
| description | Abstract Mice xenotransplanted with human cells and/or expressing human gene products (also known as “humanized mice”) recapitulate the human evolutionary specialization and diversity of genotypic and phenotypic traits. These models can provide a relevant in vivo context for understanding of human‐specific physiology and pathologies. Humanized mice have advanced toward mainstream preclinical models and are now at the forefront of biomedical research. Here, we considered innovations and challenges regarding the reconstitution of human immunity and human tissues, modeling of human infections and cancer, and the use of humanized mice for testing drugs or regenerative therapy products. As the number of publications exploring different facets of humanized mouse models has steadily increased in past years, it is becoming evident that standardized reporting is needed in the field. Therefore, an international community‐driven resource called “Minimal Information for Standardization of Humanized Mice” (MISHUM) has been created for the purpose of enhancing rigor and reproducibility of studies in the field. Within MISHUM, we propose comprehensive guidelines for reporting critical information generated using humanized mice. |
| format | Article |
| id | doaj-art-0b7a5df243a34bd59c1903d19ca29593 |
| institution | DOAJ |
| issn | 1757-4676 1757-4684 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | EMBO Molecular Medicine |
| spelling | doaj-art-0b7a5df243a34bd59c1903d19ca295932025-08-20T03:06:00ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842020-06-0112711610.15252/emmm.201708662Innovations, challenges, and minimal information for standardization of humanized miceRenata Stripecke0Christian Münz1Jan Jacob Schuringa2Karl‐Dimiter Bissig3Brian Soper4Terrence Meeham5Li‐Chin Yao6James P Di Santo7Michael Brehm8Estefania Rodriguez9Anja Kathrin Wege10Dominique Bonnet11Silvia Guionaud12Kristina E Howard13Scott Kitchen14Florian Klein15Kourosh Saeb‐Parsy16Johannes Sam17Amar Deep Sharma18Andreas Trumpp19Livio Trusolino20Carol Bult21Leonard Shultz22Regenerative Immune Therapies Applied, Hannover Medical SchoolViral Immunobiology, Institute of Experimental Immunology, University of ZurichDepartment of Hematology, University Medical Center Groningen, University of GroningenDepartment of Pediatrics, Duke University Medical CenterThe Jackson LaboratoryKymab BiotechnologyThe Jackson LaboratoryInstitut Pasteur, INSERM U1223University of Massachusetts Medical SchoolBernhard Nocht Institute for Tropical Medicine, Virology Dept.Department of Gynecology and Obstetrics, University Cancer Center RegensburgThe Francis Crick InstituteGuionaud Nonclinical ConsultingU.S. Food & Drug AdministrationUniversity of California, Los AngelesUniversity of CologneUniversity of CambridgeRoche Innovation Center ZurichRegenerative Immune Therapies Applied, Hannover Medical SchoolDivision of Stem Cells and Cancer, German Cancer Research Center (DKFZ)Department of Oncology, University of Torino Medical SchoolThe Jackson LaboratoryThe Jackson LaboratoryAbstract Mice xenotransplanted with human cells and/or expressing human gene products (also known as “humanized mice”) recapitulate the human evolutionary specialization and diversity of genotypic and phenotypic traits. These models can provide a relevant in vivo context for understanding of human‐specific physiology and pathologies. Humanized mice have advanced toward mainstream preclinical models and are now at the forefront of biomedical research. Here, we considered innovations and challenges regarding the reconstitution of human immunity and human tissues, modeling of human infections and cancer, and the use of humanized mice for testing drugs or regenerative therapy products. As the number of publications exploring different facets of humanized mouse models has steadily increased in past years, it is becoming evident that standardized reporting is needed in the field. Therefore, an international community‐driven resource called “Minimal Information for Standardization of Humanized Mice” (MISHUM) has been created for the purpose of enhancing rigor and reproducibility of studies in the field. Within MISHUM, we propose comprehensive guidelines for reporting critical information generated using humanized mice.https://doi.org/10.15252/emmm.201708662humanized miceinfectionsPDXimmuno‐oncologyregenerative medicine |
| spellingShingle | Renata Stripecke Christian Münz Jan Jacob Schuringa Karl‐Dimiter Bissig Brian Soper Terrence Meeham Li‐Chin Yao James P Di Santo Michael Brehm Estefania Rodriguez Anja Kathrin Wege Dominique Bonnet Silvia Guionaud Kristina E Howard Scott Kitchen Florian Klein Kourosh Saeb‐Parsy Johannes Sam Amar Deep Sharma Andreas Trumpp Livio Trusolino Carol Bult Leonard Shultz Innovations, challenges, and minimal information for standardization of humanized mice EMBO Molecular Medicine humanized mice infections PDX immuno‐oncology regenerative medicine |
| title | Innovations, challenges, and minimal information for standardization of humanized mice |
| title_full | Innovations, challenges, and minimal information for standardization of humanized mice |
| title_fullStr | Innovations, challenges, and minimal information for standardization of humanized mice |
| title_full_unstemmed | Innovations, challenges, and minimal information for standardization of humanized mice |
| title_short | Innovations, challenges, and minimal information for standardization of humanized mice |
| title_sort | innovations challenges and minimal information for standardization of humanized mice |
| topic | humanized mice infections PDX immuno‐oncology regenerative medicine |
| url | https://doi.org/10.15252/emmm.201708662 |
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