Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes
ObjectiveTo construct a prognostic model of lung adenocarcinoma (LUAD) based on m5C modification-related genes and to explore its clinical value. MethodsBased on the LUAD data in TCGA, GSE30219, GSE31210, and GSE50081 cohorts, prognosis-related m5C modification-related genes were screened, and the p...
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Magazine House of Cancer Research on Prevention and Treatment
2025-03-01
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| Series: | Zhongliu Fangzhi Yanjiu |
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| Online Access: | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0992 |
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| author | Fan YANG Nongyan WANG Meng FANG Yingjiao ZHANG Haiyan HU Peng FANG |
| author_facet | Fan YANG Nongyan WANG Meng FANG Yingjiao ZHANG Haiyan HU Peng FANG |
| author_sort | Fan YANG |
| collection | DOAJ |
| description | ObjectiveTo construct a prognostic model of lung adenocarcinoma (LUAD) based on m5C modification-related genes and to explore its clinical value. MethodsBased on the LUAD data in TCGA, GSE30219, GSE31210, and GSE50081 cohorts, prognosis-related m5C modification-related genes were screened, and the prognostic model was constructed by using univariate Cox, Lasso, and multivariate Cox regression analyses. Kaplan-Meier curve, ROC curve, and Cox regression were used to observe the robustness and prognostic performance of the model. The correlation between the prognostic model and clinicopathologic features was further explored. ResultsA prognostic model consisting of eight m5C modification-related genes, including CDK1, CDKN1A, NOP2, RRM2, TCL6, TLR8, TRDMT1, and YTHDF2, was constructed. Risk score was an independent risk factor for the prognosis of patients with LUAD, and it is combined with age, T stage, and N stage to constitute a nomogram which can accurately predict the prognosis of patients. The infiltration of macrophages and CD4+/CD8+T cells was significantly reduced in high-risk patients. The risk score in LUAD tissues was significantly higher than that in normal tissues and was positively correlated with T stage and N stage. The risk score of smoking and EGFR wild-type patients was higher than that of non-smoking and EGFR-mutant patients. ConclusionThe prognostic model constructed based on m5C modification-related genes has shown good accuracy and stability in predicting the prognosis of patients with LUAD, and it is closely related to clinical features, driver gene mutations, and immune infiltration, which can provide a potential basis for the treatment and prognostic assessment of LUAD. |
| format | Article |
| id | doaj-art-0b72abdec7dc4bff89056d5d5f005d01 |
| institution | DOAJ |
| issn | 1000-8578 |
| language | zho |
| publishDate | 2025-03-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj-art-0b72abdec7dc4bff89056d5d5f005d012025-08-20T02:48:39ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782025-03-0152320821610.3971/j.issn.1000-8578.2025.24.099220240992Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related GenesFan YANG0Nongyan WANG1Meng FANG2Yingjiao ZHANG3Haiyan HU4Peng FANG5Department of General Medicine, No. 903 Hospital of PLA Joint Logistic Support Force (Affiliated Xihu Hospital of Hangzhou Medical College), Hangzhou 310012, ChinaDepartment of General Medicine, No. 903 Hospital of PLA Joint Logistic Support Force (Affiliated Xihu Hospital of Hangzhou Medical College), Hangzhou 310012, ChinaDepartment of General Medicine, No. 903 Hospital of PLA Joint Logistic Support Force (Affiliated Xihu Hospital of Hangzhou Medical College), Hangzhou 310012, ChinaDepartment of General Medicine, No. 903 Hospital of PLA Joint Logistic Support Force (Affiliated Xihu Hospital of Hangzhou Medical College), Hangzhou 310012, ChinaDepartment of General Medicine, No. 903 Hospital of PLA Joint Logistic Support Force (Affiliated Xihu Hospital of Hangzhou Medical College), Hangzhou 310012, ChinaDepartment of General Medicine, No. 903 Hospital of PLA Joint Logistic Support Force (Affiliated Xihu Hospital of Hangzhou Medical College), Hangzhou 310012, ChinaObjectiveTo construct a prognostic model of lung adenocarcinoma (LUAD) based on m5C modification-related genes and to explore its clinical value. MethodsBased on the LUAD data in TCGA, GSE30219, GSE31210, and GSE50081 cohorts, prognosis-related m5C modification-related genes were screened, and the prognostic model was constructed by using univariate Cox, Lasso, and multivariate Cox regression analyses. Kaplan-Meier curve, ROC curve, and Cox regression were used to observe the robustness and prognostic performance of the model. The correlation between the prognostic model and clinicopathologic features was further explored. ResultsA prognostic model consisting of eight m5C modification-related genes, including CDK1, CDKN1A, NOP2, RRM2, TCL6, TLR8, TRDMT1, and YTHDF2, was constructed. Risk score was an independent risk factor for the prognosis of patients with LUAD, and it is combined with age, T stage, and N stage to constitute a nomogram which can accurately predict the prognosis of patients. The infiltration of macrophages and CD4+/CD8+T cells was significantly reduced in high-risk patients. The risk score in LUAD tissues was significantly higher than that in normal tissues and was positively correlated with T stage and N stage. The risk score of smoking and EGFR wild-type patients was higher than that of non-smoking and EGFR-mutant patients. ConclusionThe prognostic model constructed based on m5C modification-related genes has shown good accuracy and stability in predicting the prognosis of patients with LUAD, and it is closely related to clinical features, driver gene mutations, and immune infiltration, which can provide a potential basis for the treatment and prognostic assessment of LUAD.http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0992lung adenocarcinomamethylation modificationm5cprognosis modelrisk scorenomogram |
| spellingShingle | Fan YANG Nongyan WANG Meng FANG Yingjiao ZHANG Haiyan HU Peng FANG Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes Zhongliu Fangzhi Yanjiu lung adenocarcinoma methylation modification m5c prognosis model risk score nomogram |
| title | Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes |
| title_full | Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes |
| title_fullStr | Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes |
| title_full_unstemmed | Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes |
| title_short | Construction and Validation of A Prognostic Model of Lung Adenocarcinoma Based on m5C Modification-Related Genes |
| title_sort | construction and validation of a prognostic model of lung adenocarcinoma based on m5c modification related genes |
| topic | lung adenocarcinoma methylation modification m5c prognosis model risk score nomogram |
| url | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.0992 |
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