Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity
Abstract Nucleic acids and proteins serve as crucial biomarkers used in the diagnosis, prognosis and therapy monitoring across various diseases. Traditionally, these biomarkers are identified at an ensemble level following the amplification of target or signaling molecules. However, these methods ha...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-05-01
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| Series: | Experimental and Molecular Medicine |
| Online Access: | https://doi.org/10.1038/s12276-025-01453-w |
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| _version_ | 1850137754647658496 |
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| author | Soochul Shin Juyoung Kim Eunho Song Sun Han Sungchul Hohng |
| author_facet | Soochul Shin Juyoung Kim Eunho Song Sun Han Sungchul Hohng |
| author_sort | Soochul Shin |
| collection | DOAJ |
| description | Abstract Nucleic acids and proteins serve as crucial biomarkers used in the diagnosis, prognosis and therapy monitoring across various diseases. Traditionally, these biomarkers are identified at an ensemble level following the amplification of target or signaling molecules. However, these methods have limitations in addressing contemporary challenges in molecular diagnostics, such as low sensitivity, specificity, throughput and imprecise quantification. To overcome these limitations, various analytical techniques offering single-molecule sensitivity have been developed. Here we aim to provide a concise overview of historically notable and potentially promising analytical techniques to detect nucleic acids and proteins with single-molecule sensitivity. Our focus is on their potential in liquid biopsy, delineating their strengths and weaknesses, providing insights into their ability to revolutionize biomarker analysis and paving the way for more advanced technologies. |
| format | Article |
| id | doaj-art-0b6fdf76a10549dfa87bb39bbeb14934 |
| institution | OA Journals |
| issn | 2092-6413 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Experimental and Molecular Medicine |
| spelling | doaj-art-0b6fdf76a10549dfa87bb39bbeb149342025-08-20T02:30:44ZengNature Publishing GroupExperimental and Molecular Medicine2092-64132025-05-0157593894910.1038/s12276-025-01453-wAnalytical techniques for nucleic acid and protein detection with single-molecule sensitivitySoochul Shin0Juyoung Kim1Eunho Song2Sun Han3Sungchul Hohng4Department of Physics and Astronomy, Institute of Applied Physics, Seoul National UniversityDepartment of Physics and Astronomy, Institute of Applied Physics, Seoul National UniversityDepartment of Physics and Astronomy, Institute of Applied Physics, Seoul National UniversityDepartment of Physics and Astronomy, Institute of Applied Physics, Seoul National UniversityDepartment of Physics and Astronomy, Institute of Applied Physics, Seoul National UniversityAbstract Nucleic acids and proteins serve as crucial biomarkers used in the diagnosis, prognosis and therapy monitoring across various diseases. Traditionally, these biomarkers are identified at an ensemble level following the amplification of target or signaling molecules. However, these methods have limitations in addressing contemporary challenges in molecular diagnostics, such as low sensitivity, specificity, throughput and imprecise quantification. To overcome these limitations, various analytical techniques offering single-molecule sensitivity have been developed. Here we aim to provide a concise overview of historically notable and potentially promising analytical techniques to detect nucleic acids and proteins with single-molecule sensitivity. Our focus is on their potential in liquid biopsy, delineating their strengths and weaknesses, providing insights into their ability to revolutionize biomarker analysis and paving the way for more advanced technologies.https://doi.org/10.1038/s12276-025-01453-w |
| spellingShingle | Soochul Shin Juyoung Kim Eunho Song Sun Han Sungchul Hohng Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity Experimental and Molecular Medicine |
| title | Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity |
| title_full | Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity |
| title_fullStr | Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity |
| title_full_unstemmed | Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity |
| title_short | Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity |
| title_sort | analytical techniques for nucleic acid and protein detection with single molecule sensitivity |
| url | https://doi.org/10.1038/s12276-025-01453-w |
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