Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasma
Liver diseases have become widespread especially due to various factors of modern life. Although the effect of N-acetyl-L-cysteine (NAC) is investigated in the recovery of liver damage, gas plasma therapy can be identified as a promising candidate. Our study aimed to enhance the effectiveness of ine...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324017184 |
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| author | Masume Farhadi Farshad Sohbatzadeh Akbar Hajizadeh Moghaddam Yasaman Firouzjaei Cheng Cheng |
| author_facet | Masume Farhadi Farshad Sohbatzadeh Akbar Hajizadeh Moghaddam Yasaman Firouzjaei Cheng Cheng |
| author_sort | Masume Farhadi |
| collection | DOAJ |
| description | Liver diseases have become widespread especially due to various factors of modern life. Although the effect of N-acetyl-L-cysteine (NAC) is investigated in the recovery of liver damage, gas plasma therapy can be identified as a promising candidate. Our study aimed to enhance the effectiveness of ineffective doses of NAC in stopping CCl4-induced hepatotoxicity in rats by physical cold plasma. The plasma-treated NAC (PTN) structural changes were investigated through FTIR and LCMS/MS analysis. It was observed that the PTN consists of various chemical bioproducts with different molecular weights. We investigated an ineffective dose of NAC and its parallel effect through the administration of PTN on liver and kidney morphology and several biochemical factors including ALT, AST, and ALP. Additionally, we examined oxidative stress, antioxidant parameters, and glutathione (GSH) levels. Results showed that PTN exhibited greater antioxidant properties and increased GSH levels, contributing to its therapeutic effects. Also, the antioxidant enzymes and oxidative stress activities improved after receiving PTN. It also enhanced histological parameters, although various damages were detected in both liver and kidney tissues after CCl4 injection, PTN remarkably prevented the tissue changes caused by CCl4. PTN could protect against liver damage even at a very low dose of NAC, acting as a prophylactic drug with a high margin of safety for hepatotoxicity. |
| format | Article |
| id | doaj-art-0b691f72502d47588282cafa53ced587 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-0b691f72502d47588282cafa53ced5872025-01-23T05:25:56ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117642Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasmaMasume Farhadi0Farshad Sohbatzadeh1Akbar Hajizadeh Moghaddam2Yasaman Firouzjaei3Cheng Cheng4Department of Atomic and Molecular Physics, Faculty of Science, University of Mazandaran, Babolsar, IranDepartment of Atomic and Molecular Physics, Faculty of Science, University of Mazandaran, Babolsar, Iran; Plasma Technology Research Core, Faculty of Science, University of Mazandaran, Babolsar, Iran; Corresponding author at: Department of Atomic and Molecular Physics, Faculty of Science, University of Mazandaran, Babolsar, Iran.Department of Animal Science, Faculty of Science, University of Mazandaran, Babolsar, IranDepartment of Animal Science, Faculty of Science, University of Mazandaran, Babolsar, IranInstitute of Plasma Physics, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaLiver diseases have become widespread especially due to various factors of modern life. Although the effect of N-acetyl-L-cysteine (NAC) is investigated in the recovery of liver damage, gas plasma therapy can be identified as a promising candidate. Our study aimed to enhance the effectiveness of ineffective doses of NAC in stopping CCl4-induced hepatotoxicity in rats by physical cold plasma. The plasma-treated NAC (PTN) structural changes were investigated through FTIR and LCMS/MS analysis. It was observed that the PTN consists of various chemical bioproducts with different molecular weights. We investigated an ineffective dose of NAC and its parallel effect through the administration of PTN on liver and kidney morphology and several biochemical factors including ALT, AST, and ALP. Additionally, we examined oxidative stress, antioxidant parameters, and glutathione (GSH) levels. Results showed that PTN exhibited greater antioxidant properties and increased GSH levels, contributing to its therapeutic effects. Also, the antioxidant enzymes and oxidative stress activities improved after receiving PTN. It also enhanced histological parameters, although various damages were detected in both liver and kidney tissues after CCl4 injection, PTN remarkably prevented the tissue changes caused by CCl4. PTN could protect against liver damage even at a very low dose of NAC, acting as a prophylactic drug with a high margin of safety for hepatotoxicity.http://www.sciencedirect.com/science/article/pii/S0147651324017184Cold atmospheric pressure plasmaN-acetyl-L-cysteineReactive oxygen and nitrogen speciesCCl4-induced hepatotoxicity |
| spellingShingle | Masume Farhadi Farshad Sohbatzadeh Akbar Hajizadeh Moghaddam Yasaman Firouzjaei Cheng Cheng Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasma Ecotoxicology and Environmental Safety Cold atmospheric pressure plasma N-acetyl-L-cysteine Reactive oxygen and nitrogen species CCl4-induced hepatotoxicity |
| title | Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasma |
| title_full | Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasma |
| title_fullStr | Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasma |
| title_full_unstemmed | Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasma |
| title_short | Enhancing the efficacy of low doses of N-acetyl-L-cysteine in mitigating CCl4-induced hepatotoxicity in animal model using physical cold plasma |
| title_sort | enhancing the efficacy of low doses of n acetyl l cysteine in mitigating ccl4 induced hepatotoxicity in animal model using physical cold plasma |
| topic | Cold atmospheric pressure plasma N-acetyl-L-cysteine Reactive oxygen and nitrogen species CCl4-induced hepatotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324017184 |
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