Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant Activities
The unique properties of silver nanoparticles (AgNPs), such as size, surface charge, and the ability to release silver ions, contribute to DNA damage, inducing of oxidative stress, and apoptosis in cancer cells. Thus, the potential application of AgNPs in the field of biomedicine, and cancer therapy...
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2024-11-01
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| author | Dandan Wang Haijing Ke Hongtao Wang Jingyu Shen Yan Jin Bo Lu Bingju Wang Shuang Li Yao Li Wan Taek Im Muhammad Zubair Siddiqi Haibo Zhu |
| author_facet | Dandan Wang Haijing Ke Hongtao Wang Jingyu Shen Yan Jin Bo Lu Bingju Wang Shuang Li Yao Li Wan Taek Im Muhammad Zubair Siddiqi Haibo Zhu |
| author_sort | Dandan Wang |
| collection | DOAJ |
| description | The unique properties of silver nanoparticles (AgNPs), such as size, surface charge, and the ability to release silver ions, contribute to DNA damage, inducing of oxidative stress, and apoptosis in cancer cells. Thus, the potential application of AgNPs in the field of biomedicine, and cancer therapy are rapidly increasing day by day. Therefore, in this study, AgNPs were synthesized by extract of <i>Chuanminshen violaceum</i>, and then the synthesized CM-AgNPs were fully characterized. The biological activity of CM-AgNPs was investigated for antibacterial, antioxidant, and anticancer activities. The cytotoxic activity of CM-AgNPs was tested for various kinds of cancer cells including MKN45 gastric cancer cells, HCT116 human colon cancer cells, A549 human lung cancer cells, and HepG2 liver cancer cells. Among these cancer cells, the induced apoptosis activity of CM-AgNPs on HCT116 cancer cells was better and was used for further investigation. Besides, the CM-AgNPs exhibited great antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with 50% free radical scavenging activity, and CM-AgNPs also showed a significant antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Thus, our pilot data demonstrated that the green synthesis of CM-AgNPs would be considered a good candidate for the treatment of HCT116 cancer cells, with its strong antioxidant activity and antibacterial effects. |
| format | Article |
| id | doaj-art-0b5f49b2879b4745a408183df6763bd1 |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Molecules |
| spelling | doaj-art-0b5f49b2879b4745a408183df6763bd12025-08-20T01:55:45ZengMDPI AGMolecules1420-30492024-11-012923568210.3390/molecules29235682Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant ActivitiesDandan Wang0Haijing Ke1Hongtao Wang2Jingyu Shen3Yan Jin4Bo Lu5Bingju Wang6Shuang Li7Yao Li8Wan Taek Im9Muhammad Zubair Siddiqi10Haibo Zhu11College of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaSchool of Life Science, Nantong University, Nantong 226019, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaDepartment of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si 17579, Gyeonggi-do, Republic of KoreaDepartment of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si 17579, Gyeonggi-do, Republic of KoreaSchool of Public Health and Management, Binzhou Medical University, Yantai 264005, ChinaThe unique properties of silver nanoparticles (AgNPs), such as size, surface charge, and the ability to release silver ions, contribute to DNA damage, inducing of oxidative stress, and apoptosis in cancer cells. Thus, the potential application of AgNPs in the field of biomedicine, and cancer therapy are rapidly increasing day by day. Therefore, in this study, AgNPs were synthesized by extract of <i>Chuanminshen violaceum</i>, and then the synthesized CM-AgNPs were fully characterized. The biological activity of CM-AgNPs was investigated for antibacterial, antioxidant, and anticancer activities. The cytotoxic activity of CM-AgNPs was tested for various kinds of cancer cells including MKN45 gastric cancer cells, HCT116 human colon cancer cells, A549 human lung cancer cells, and HepG2 liver cancer cells. Among these cancer cells, the induced apoptosis activity of CM-AgNPs on HCT116 cancer cells was better and was used for further investigation. Besides, the CM-AgNPs exhibited great antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with 50% free radical scavenging activity, and CM-AgNPs also showed a significant antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Thus, our pilot data demonstrated that the green synthesis of CM-AgNPs would be considered a good candidate for the treatment of HCT116 cancer cells, with its strong antioxidant activity and antibacterial effects.https://www.mdpi.com/1420-3049/29/23/5682<i>Chuanminshen violaceum Sheh et Shan</i>green synthesissilver nanoparticlesanti-oxidant activitybacteriostasisanti-cancer properties |
| spellingShingle | Dandan Wang Haijing Ke Hongtao Wang Jingyu Shen Yan Jin Bo Lu Bingju Wang Shuang Li Yao Li Wan Taek Im Muhammad Zubair Siddiqi Haibo Zhu Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant Activities Molecules <i>Chuanminshen violaceum Sheh et Shan</i> green synthesis silver nanoparticles anti-oxidant activity bacteriostasis anti-cancer properties |
| title | Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant Activities |
| title_full | Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant Activities |
| title_fullStr | Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant Activities |
| title_full_unstemmed | Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant Activities |
| title_short | Green Synthesis of Silver Nanoparticles (CM-AgNPs) from the Root of <i>Chuanminshen</i> for Improving the Cytotoxicity Effect in Cancer Cells with Antibacterial and Antioxidant Activities |
| title_sort | green synthesis of silver nanoparticles cm agnps from the root of i chuanminshen i for improving the cytotoxicity effect in cancer cells with antibacterial and antioxidant activities |
| topic | <i>Chuanminshen violaceum Sheh et Shan</i> green synthesis silver nanoparticles anti-oxidant activity bacteriostasis anti-cancer properties |
| url | https://www.mdpi.com/1420-3049/29/23/5682 |
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