Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells.
Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labe...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179618&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849766157879345152 |
|---|---|
| author | Jie Chen Wei Guo Xiaoqun Dang Yukang Huang Fangyan Liu Xianzhi Meng Yaoyao An Mengxian Long Jialing Bao Zeyang Zhou Zhonghuai Xiang Guoqing Pan |
| author_facet | Jie Chen Wei Guo Xiaoqun Dang Yukang Huang Fangyan Liu Xianzhi Meng Yaoyao An Mengxian Long Jialing Bao Zeyang Zhou Zhonghuai Xiang Guoqing Pan |
| author_sort | Jie Chen |
| collection | DOAJ |
| description | Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia Nosema bombycis in host cells. Based on the presence of chitin, Calcofluor White M2R was used to label the sporogonic phase, while β-tubulin antibody coupled with fluorescence secondary antibody were used to label the proliferative phase by immunofluorescence. This method is simple, efficient and can be used on both infected cells and tissue slices, providing a great potential application in microsporidia research. |
| format | Article |
| id | doaj-art-0b4ac59de5df47d7b59eb7799fffec4a |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-0b4ac59de5df47d7b59eb7799fffec4a2025-08-20T03:04:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017961810.1371/journal.pone.0179618Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells.Jie ChenWei GuoXiaoqun DangYukang HuangFangyan LiuXianzhi MengYaoyao AnMengxian LongJialing BaoZeyang ZhouZhonghuai XiangGuoqing PanMicrosporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia Nosema bombycis in host cells. Based on the presence of chitin, Calcofluor White M2R was used to label the sporogonic phase, while β-tubulin antibody coupled with fluorescence secondary antibody were used to label the proliferative phase by immunofluorescence. This method is simple, efficient and can be used on both infected cells and tissue slices, providing a great potential application in microsporidia research.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179618&type=printable |
| spellingShingle | Jie Chen Wei Guo Xiaoqun Dang Yukang Huang Fangyan Liu Xianzhi Meng Yaoyao An Mengxian Long Jialing Bao Zeyang Zhou Zhonghuai Xiang Guoqing Pan Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells. PLoS ONE |
| title | Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells. |
| title_full | Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells. |
| title_fullStr | Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells. |
| title_full_unstemmed | Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells. |
| title_short | Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells. |
| title_sort | easy labeling of proliferative phase and sporogonic phase of microsporidia nosema bombycis in host cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179618&type=printable |
| work_keys_str_mv | AT jiechen easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT weiguo easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT xiaoqundang easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT yukanghuang easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT fangyanliu easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT xianzhimeng easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT yaoyaoan easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT mengxianlong easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT jialingbao easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT zeyangzhou easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT zhonghuaixiang easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells AT guoqingpan easylabelingofproliferativephaseandsporogonicphaseofmicrosporidianosemabombycisinhostcells |