Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming
This study delves into the therapeutic potential of β-hydroxybutyrate (BHB) in clear cell renal cell carcinoma (ccRCC), a cancer known for its complex pathogenesis and resistance to conventional treatments. The research specifically explores the impact of BHB on cell viability, autophagy induction,...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Clinical Practice |
| Online Access: | http://dx.doi.org/10.1155/2024/8805868 |
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| author | Roya Rezaei Asra Abdali Larki Rosa Hosseinzadegan Zahra Dashti Saba Tarkashvand Reihaneh Akhoondi Morvarid Siri Mesbah Shams Alireza Monsef Sanaz Dastghaib |
| author_facet | Roya Rezaei Asra Abdali Larki Rosa Hosseinzadegan Zahra Dashti Saba Tarkashvand Reihaneh Akhoondi Morvarid Siri Mesbah Shams Alireza Monsef Sanaz Dastghaib |
| author_sort | Roya Rezaei |
| collection | DOAJ |
| description | This study delves into the therapeutic potential of β-hydroxybutyrate (BHB) in clear cell renal cell carcinoma (ccRCC), a cancer known for its complex pathogenesis and resistance to conventional treatments. The research specifically explores the impact of BHB on cell viability, autophagy induction, and lipid metabolism in Caki-1 cells. The findings reveal that BHB significantly reduces ccRCC cell viability, particularly under low-glucose conditions. The combination of glucose and BHB treatment activates autophagy pathways, as evidenced by increased expression of autophagy-related genes (Beclin-1, LC3IIβ, and ATG5) and decreased expression of P62 after 48 and 72 hours. Moreover, the combined therapy enhances lipid metabolism, as indicated by elevated expression of PGC-1α and UCP-1, along with upregulation of ACSL3 and CPT1A, which are associated with lipid droplet formation and facilitate lipid breakdown within cells. The study concludes that BHB holds promise as a therapeutic agent for ccRCC, targeting abnormal lipid metabolism, inducing autophagy-mediated cell death, and promoting fat browning. The results suggest potential avenues for precision-guided nutritional therapies in ccRCC treatment, highlighting the innovative role of BHB in addressing the challenges posed by this cancer. |
| format | Article |
| id | doaj-art-0b40397d27b94fb1a09a3cdadd7f1ec5 |
| institution | OA Journals |
| issn | 1742-1241 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Clinical Practice |
| spelling | doaj-art-0b40397d27b94fb1a09a3cdadd7f1ec52025-08-20T02:03:43ZengWileyInternational Journal of Clinical Practice1742-12412024-01-01202410.1155/2024/8805868Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor SlimmingRoya Rezaei0Asra Abdali Larki1Rosa Hosseinzadegan2Zahra Dashti3Saba Tarkashvand4Reihaneh Akhoondi5Morvarid Siri6Mesbah Shams7Alireza Monsef8Sanaz Dastghaib9Department of MicrobiologyDepartment of BiologyDepartment of BiologyDepartment of GeneticsSina Surgery Research CenterUniversal Scientific Education and Research Network (USERN)Autophagy Research CenterEndocrinology and Metabolism Research CenterFasa University of Medical SciencesAutophagy Research CenterThis study delves into the therapeutic potential of β-hydroxybutyrate (BHB) in clear cell renal cell carcinoma (ccRCC), a cancer known for its complex pathogenesis and resistance to conventional treatments. The research specifically explores the impact of BHB on cell viability, autophagy induction, and lipid metabolism in Caki-1 cells. The findings reveal that BHB significantly reduces ccRCC cell viability, particularly under low-glucose conditions. The combination of glucose and BHB treatment activates autophagy pathways, as evidenced by increased expression of autophagy-related genes (Beclin-1, LC3IIβ, and ATG5) and decreased expression of P62 after 48 and 72 hours. Moreover, the combined therapy enhances lipid metabolism, as indicated by elevated expression of PGC-1α and UCP-1, along with upregulation of ACSL3 and CPT1A, which are associated with lipid droplet formation and facilitate lipid breakdown within cells. The study concludes that BHB holds promise as a therapeutic agent for ccRCC, targeting abnormal lipid metabolism, inducing autophagy-mediated cell death, and promoting fat browning. The results suggest potential avenues for precision-guided nutritional therapies in ccRCC treatment, highlighting the innovative role of BHB in addressing the challenges posed by this cancer.http://dx.doi.org/10.1155/2024/8805868 |
| spellingShingle | Roya Rezaei Asra Abdali Larki Rosa Hosseinzadegan Zahra Dashti Saba Tarkashvand Reihaneh Akhoondi Morvarid Siri Mesbah Shams Alireza Monsef Sanaz Dastghaib Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming International Journal of Clinical Practice |
| title | Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming |
| title_full | Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming |
| title_fullStr | Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming |
| title_full_unstemmed | Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming |
| title_short | Exploring the Therapeutic Potential of β-Hydroxybutyrate (BHB) in Clear Cell Renal Cell Carcinoma: A Journey into Fat Browning, Autophagy, and Tumor Slimming |
| title_sort | exploring the therapeutic potential of β hydroxybutyrate bhb in clear cell renal cell carcinoma a journey into fat browning autophagy and tumor slimming |
| url | http://dx.doi.org/10.1155/2024/8805868 |
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