Establishment of a novel engineering breeding technology for cytoplasmic male sterility
The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration modu...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-08-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514125001096 |
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| author | Na Xu Dong Yu Longhui Zhang Tiankang Wang Zhongxiao Hu Quanqiang Lei Yingxin Qiu Zheming Yuan Shufeng Song Li Li |
| author_facet | Na Xu Dong Yu Longhui Zhang Tiankang Wang Zhongxiao Hu Quanqiang Lei Yingxin Qiu Zheming Yuan Shufeng Song Li Li |
| author_sort | Na Xu |
| collection | DOAJ |
| description | The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20, sorting marker gene DsRed2, and pollen lethal gene ZmAA1, then transformed this into a CMS line. We obtained reproductive lines without alterations to main agronomic traits, which can produce engineering sterile lines (ESL) and reproductive line seeds at a 1:1 ratio by self-crossing. CEBT converts the traditional three-line system into an efficient ‘new two-line’ hybrid framework: the reproductive line is improved while simultaneously improving the ESL, which significantly shortens the breeding process for three-line hybrid rice. |
| format | Article |
| id | doaj-art-0b36d908096a487c8a3fc6af148f2d23 |
| institution | Kabale University |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-0b36d908096a487c8a3fc6af148f2d232025-08-22T04:56:25ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-08-011341305131110.1016/j.cj.2025.04.010Establishment of a novel engineering breeding technology for cytoplasmic male sterilityNa Xu0Dong Yu1Longhui Zhang2Tiankang Wang3Zhongxiao Hu4Quanqiang Lei5Yingxin Qiu6Zheming Yuan7Shufeng Song8Li Li9Hunan Engineering & Technology Research Centre for Agricultural Big Data Analysis & Decision-making, College of Plant Protection, Hunan Agricultural University, 410128 Changsha, Hunan, China; Yuelushan Laboratory, Changsha 410128, Hunan, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, China; Yuelushan Laboratory, Changsha 410128, Hunan, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, ChinaLongping Branch, College of Biology, Hunan University, Changsha 410125, Hunan, ChinaLongping Branch, College of Biology, Hunan University, Changsha 410125, Hunan, ChinaHunan Engineering & Technology Research Centre for Agricultural Big Data Analysis & Decision-making, College of Plant Protection, Hunan Agricultural University, 410128 Changsha, Hunan, China; Corresponding authors.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, China; Yuelushan Laboratory, Changsha 410128, Hunan, China; Longping Branch, College of Biology, Hunan University, Changsha 410125, Hunan, China; Corresponding authors.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, China; Yuelushan Laboratory, Changsha 410128, Hunan, China; Longping Branch, College of Biology, Hunan University, Changsha 410125, Hunan, China; Corresponding authors.The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20, sorting marker gene DsRed2, and pollen lethal gene ZmAA1, then transformed this into a CMS line. We obtained reproductive lines without alterations to main agronomic traits, which can produce engineering sterile lines (ESL) and reproductive line seeds at a 1:1 ratio by self-crossing. CEBT converts the traditional three-line system into an efficient ‘new two-line’ hybrid framework: the reproductive line is improved while simultaneously improving the ESL, which significantly shortens the breeding process for three-line hybrid rice.http://www.sciencedirect.com/science/article/pii/S2214514125001096Three-line hybrid riceCMS-WARf geneCEBTReproductive lines |
| spellingShingle | Na Xu Dong Yu Longhui Zhang Tiankang Wang Zhongxiao Hu Quanqiang Lei Yingxin Qiu Zheming Yuan Shufeng Song Li Li Establishment of a novel engineering breeding technology for cytoplasmic male sterility Crop Journal Three-line hybrid rice CMS-WA Rf gene CEBT Reproductive lines |
| title | Establishment of a novel engineering breeding technology for cytoplasmic male sterility |
| title_full | Establishment of a novel engineering breeding technology for cytoplasmic male sterility |
| title_fullStr | Establishment of a novel engineering breeding technology for cytoplasmic male sterility |
| title_full_unstemmed | Establishment of a novel engineering breeding technology for cytoplasmic male sterility |
| title_short | Establishment of a novel engineering breeding technology for cytoplasmic male sterility |
| title_sort | establishment of a novel engineering breeding technology for cytoplasmic male sterility |
| topic | Three-line hybrid rice CMS-WA Rf gene CEBT Reproductive lines |
| url | http://www.sciencedirect.com/science/article/pii/S2214514125001096 |
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