Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR Study
The subgingival microbiome is a critical component of the oral microbiota and plays a central role in pediatric periodontology. This study investigated differences in periodontopathogen profiles in children with gingival inflammation compared to healthy controls using real-time PCR, with a focus on...
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MDPI AG
2025-07-01
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| author | Hristina Tankova Nadezhda Mitova |
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| description | The subgingival microbiome is a critical component of the oral microbiota and plays a central role in pediatric periodontology. This study investigated differences in periodontopathogen profiles in children with gingival inflammation compared to healthy controls using real-time PCR, with a focus on the microbial complexes defined by Socransky. A total of 73 children (ages 10–14) underwent comprehensive periodontal assessment, including assessments of general health status, the O’Leary hygiene index (HI), gingival condition, and the papillary bleeding index (PBI). Subgingival plaque samples were analyzed using real-time PCR to identify key bacterial species associated with gingival health and disease. Highly pathogenic periodontopathogens such as <i>Aggregatibacter actinomycetemcomitans</i>, <i>Porphyromonas gingivalis</i>, <i>Treponema denticola</i>, and <i>Eubacterium nodatum</i> were absent in healthy subjects. In contrast, <i>Tannerella forsythia</i> was significantly more frequently detected in children with gingival inflammation (<i>p</i> < 0.05). The most abundant species in the inflammation group were <i>Prevotella intermedia</i> and <i>Capnocytophaga gingivalis.</i> Children with gingival inflammation exhibit a distinct subgingival microbiome profile characterized by an increased presence of specific periodontopathogens, including a higher prevalence of red complex species as defined by Socransky. However, the cross-sectional nature of this study limits the ability to establish causal relationships. |
| format | Article |
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| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-0b2ac761f2eb4d41b20511775abf8f572025-08-20T02:47:17ZengMDPI AGMicroorganisms2076-26072025-07-01137165610.3390/microorganisms13071656Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR StudyHristina Tankova0Nadezhda Mitova1Department of Pediatric Dental Medicine, Faculty of Dental Medicine, Medical University, 1431 Sofia, BulgariaDepartment of Pediatric Dental Medicine, Faculty of Dental Medicine, Medical University, 1431 Sofia, BulgariaThe subgingival microbiome is a critical component of the oral microbiota and plays a central role in pediatric periodontology. This study investigated differences in periodontopathogen profiles in children with gingival inflammation compared to healthy controls using real-time PCR, with a focus on the microbial complexes defined by Socransky. A total of 73 children (ages 10–14) underwent comprehensive periodontal assessment, including assessments of general health status, the O’Leary hygiene index (HI), gingival condition, and the papillary bleeding index (PBI). Subgingival plaque samples were analyzed using real-time PCR to identify key bacterial species associated with gingival health and disease. Highly pathogenic periodontopathogens such as <i>Aggregatibacter actinomycetemcomitans</i>, <i>Porphyromonas gingivalis</i>, <i>Treponema denticola</i>, and <i>Eubacterium nodatum</i> were absent in healthy subjects. In contrast, <i>Tannerella forsythia</i> was significantly more frequently detected in children with gingival inflammation (<i>p</i> < 0.05). The most abundant species in the inflammation group were <i>Prevotella intermedia</i> and <i>Capnocytophaga gingivalis.</i> Children with gingival inflammation exhibit a distinct subgingival microbiome profile characterized by an increased presence of specific periodontopathogens, including a higher prevalence of red complex species as defined by Socransky. However, the cross-sectional nature of this study limits the ability to establish causal relationships.https://www.mdpi.com/2076-2607/13/7/1656microbiomechildrenperiodontopathogensgingival inflammation |
| spellingShingle | Hristina Tankova Nadezhda Mitova Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR Study Microorganisms microbiome children periodontopathogens gingival inflammation |
| title | Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR Study |
| title_full | Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR Study |
| title_fullStr | Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR Study |
| title_full_unstemmed | Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR Study |
| title_short | Subgingival Microbiome Profiles in Bulgarian Children Aged 10–14 with Gingival Inflammation and Healthy Periodontium: A Targeted PCR Study |
| title_sort | subgingival microbiome profiles in bulgarian children aged 10 14 with gingival inflammation and healthy periodontium a targeted pcr study |
| topic | microbiome children periodontopathogens gingival inflammation |
| url | https://www.mdpi.com/2076-2607/13/7/1656 |
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