Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatiles
Background. An important consumer trait of strawberry fruits is their aroma. Methyl anthranilate makes a considerable contribution to the fruit flavor. The methyl anthranilate content in strawberry fruits is controlled by the FanAAMT (аnthranilic acid methyltransferase) gene. Identification of genet...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2021-07-01
|
| Series: | Труды по прикладной ботанике, генетике и селекции |
| Subjects: | |
| Online Access: | https://elpub.vir.nw.ru/jour/article/view/956 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545005484376064 |
|---|---|
| author | A. S. Lyzhin I. V. Luk’yanchuk |
| author_facet | A. S. Lyzhin I. V. Luk’yanchuk |
| author_sort | A. S. Lyzhin |
| collection | DOAJ |
| description | Background. An important consumer trait of strawberry fruits is their aroma. Methyl anthranilate makes a considerable contribution to the fruit flavor. The methyl anthranilate content in strawberry fruits is controlled by the FanAAMT (аnthranilic acid methyltransferase) gene. Identification of genetic determinants for this trait facilitates targeted selection of promising forms based on diagnostic DNA markers. The purpose of this study was to study the allelic diversity of the FanAAMT fruit flavor gene in wild strawberry species and strawberry cultivars for identification of promising genotypes.Materials and methods. The objects of this study were wild species of Fragaria L. as well as F. × anaschata Kantor. and F. × ananassa Duch. cultivars of different ecogeographic origin. The FanAAMT gene was identified with the dominant marker FanAAMT.Results and conclusion. In the analyzed collection of strawberry genotypes, the FanAAMT gene was identified in 36.4% of the forms, including the wild species F. vesca L., F. moschata Duch. and F. virginiana subsp. platypetala (Rydb.) Staudt, and cv. ‘Kupchikha’ (F. × anaschata). Among the analyzed F. × ananassa cultivars, the FanAAMT gene was found in 31.6% of the tested forms, specifically in 27.3% of the 22 Russian cultivars, and 37.5% of the analyzed foreign ones. Promising sources of high methyl anthranilate content in fruits were identified: wild spp. F. moschata, F. vesca, and F. virginiana subsp. platypetala; cv. ‘Kupchikha’ (F. × anaschata); Russian garden strawberry cvs. ‘Bylinnaya’, ‘Zenit’, ‘Lastochka’, ‘Neznakomka’, ‘Feyerverk’ and ‘Tsarskoselskaya’; and foreign garden strawberry cvs. ‘Karmen’, ‘Ostara’, ‘Samson’, ‘Symphony’, ‘Troubadour’ and ‘Vima Tarda’, in which the functional allele of the FanAAMT gene was found. In the remaining studied strawberry genotypes the marker FanAAMT was not detected, which presumably indicates that the FanAAMT gene is absent. cells (R9.4.1). |
| format | Article |
| id | doaj-art-0b271c1777684798b4858193877bc935 |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-0b271c1777684798b4858193877bc9352025-02-03T08:31:14ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822021-07-011822728010.30901/2227-8834-2021-2-72-80522Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatilesA. S. Lyzhin0I. V. Luk’yanchuk1I.V. Michurin Federal Science CenterI.V. Michurin Federal Science CenterBackground. An important consumer trait of strawberry fruits is their aroma. Methyl anthranilate makes a considerable contribution to the fruit flavor. The methyl anthranilate content in strawberry fruits is controlled by the FanAAMT (аnthranilic acid methyltransferase) gene. Identification of genetic determinants for this trait facilitates targeted selection of promising forms based on diagnostic DNA markers. The purpose of this study was to study the allelic diversity of the FanAAMT fruit flavor gene in wild strawberry species and strawberry cultivars for identification of promising genotypes.Materials and methods. The objects of this study were wild species of Fragaria L. as well as F. × anaschata Kantor. and F. × ananassa Duch. cultivars of different ecogeographic origin. The FanAAMT gene was identified with the dominant marker FanAAMT.Results and conclusion. In the analyzed collection of strawberry genotypes, the FanAAMT gene was identified in 36.4% of the forms, including the wild species F. vesca L., F. moschata Duch. and F. virginiana subsp. platypetala (Rydb.) Staudt, and cv. ‘Kupchikha’ (F. × anaschata). Among the analyzed F. × ananassa cultivars, the FanAAMT gene was found in 31.6% of the tested forms, specifically in 27.3% of the 22 Russian cultivars, and 37.5% of the analyzed foreign ones. Promising sources of high methyl anthranilate content in fruits were identified: wild spp. F. moschata, F. vesca, and F. virginiana subsp. platypetala; cv. ‘Kupchikha’ (F. × anaschata); Russian garden strawberry cvs. ‘Bylinnaya’, ‘Zenit’, ‘Lastochka’, ‘Neznakomka’, ‘Feyerverk’ and ‘Tsarskoselskaya’; and foreign garden strawberry cvs. ‘Karmen’, ‘Ostara’, ‘Samson’, ‘Symphony’, ‘Troubadour’ and ‘Vima Tarda’, in which the functional allele of the FanAAMT gene was found. In the remaining studied strawberry genotypes the marker FanAAMT was not detected, which presumably indicates that the FanAAMT gene is absent. cells (R9.4.1).https://elpub.vir.nw.ru/jour/article/view/956fruit flavormethyl anthranilatemolecular markersgenotype |
| spellingShingle | A. S. Lyzhin I. V. Luk’yanchuk Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatiles Труды по прикладной ботанике, генетике и селекции fruit flavor methyl anthranilate molecular markers genotype |
| title | Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatiles |
| title_full | Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatiles |
| title_fullStr | Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatiles |
| title_full_unstemmed | Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatiles |
| title_short | Genetic diversity in wild species and cultivars of strawberry for the <i>FanAAMT</i> gene controlling fruit flavor volatiles |
| title_sort | genetic diversity in wild species and cultivars of strawberry for the i fanaamt i gene controlling fruit flavor volatiles |
| topic | fruit flavor methyl anthranilate molecular markers genotype |
| url | https://elpub.vir.nw.ru/jour/article/view/956 |
| work_keys_str_mv | AT aslyzhin geneticdiversityinwildspeciesandcultivarsofstrawberryfortheifanaamtigenecontrollingfruitflavorvolatiles AT ivlukyanchuk geneticdiversityinwildspeciesandcultivarsofstrawberryfortheifanaamtigenecontrollingfruitflavorvolatiles |