Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology
【Objective】The study aims to explore the composition, sequence number and diversity of microbial community in Zhuhai Wailingding Marine Ranch, analyze the sensitivity and reducing power of eDNA technology, with a view to providing references for the management and maintenance of ecosystem in Zhuhai...
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| Format: | Article |
| Language: | English |
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Guangdong Academy of Agricultural Sciences
2023-02-01
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| Series: | Guangdong nongye kexue |
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| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202302008 |
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| author | Nengwei ZANG Aiyi ZHU Pimao CHEN Huarong YUAN Honghui ZHANG Wendi WEI |
| author_facet | Nengwei ZANG Aiyi ZHU Pimao CHEN Huarong YUAN Honghui ZHANG Wendi WEI |
| author_sort | Nengwei ZANG |
| collection | DOAJ |
| description | 【Objective】The study aims to explore the composition, sequence number and diversity of microbial community in Zhuhai Wailingding Marine Ranch, analyze the sensitivity and reducing power of eDNA technology, with a view to providing references for the management and maintenance of ecosystem in Zhuhai Wailingding Marine Ranch.【Method】In September 2021, 9 stations were set up in Zhuhai Wailingding Marine Ranch to sample at 2 m below the surface water for pretreatment, DNA extraction, PCR amplification, and high-throughput sequencing to analyze the composition of microbial species, the density and abundance of microbial resources, and determine the dominant species.【Result】A total of 30 species of microorganisms in Zhuhai Wailingding Marine Ranch were detected by eDNA technology, and 5 phyla, 7 classes, 14 orders, 19 families and 26 genera were identified, and a total of 779 706 Operational Taxonomic Units (OTUS) were detected. The Proteobacteria was the absolute dominant phylum accounting for 75.06%, followed by Bacteroidetes (11.35%), Firmicutes (5.28%), Planctomycetes (1.91%), and Actinobacteria (1.06%). There were 7 dominant species, which were Pseudoalteromonas, Marinobacter, Moraxella, Psychrobacter, Vibrio alginolyticus, Halomonas and Sphingobacterium. The dominance of Vibrio alginolyticus reached 0.19, which was the absolute dominant species, followed by Moraxella and Pseudoalteromonas reaching 0.134 and 0.127, respectively, which were the main dominant species.【Conclusion】The study reveals the microbial community information of the Zhuhai Wailingding Marine Ranch, explores the application of eDNA technology in marine ranch, and verifies the efficiency and sensitivity of this technology, providing basic data and scientific reference for future research on marine ranching microorganisms. |
| format | Article |
| id | doaj-art-0b23e30381da4e15862b34749f76e4da |
| institution | Kabale University |
| issn | 1004-874X |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Guangdong Academy of Agricultural Sciences |
| record_format | Article |
| series | Guangdong nongye kexue |
| spelling | doaj-art-0b23e30381da4e15862b34749f76e4da2025-08-20T03:48:14ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2023-02-01502677510.16768/j.issn.1004-874X.2023.02.008202302008Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA TechnologyNengwei ZANG0Aiyi ZHU1Pimao CHEN2Huarong YUAN3Honghui ZHANG4Wendi WEI5National Marine Facility Aquaculture Engineering Technology Research Center, Zhejiang Ocean University, Zhoushan 316022, ChinaNational Marine Facility Aquaculture Engineering Technology Research Center, Zhejiang Ocean University, Zhoushan 316022, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, ChinaNational Marine Facility Aquaculture Engineering Technology Research Center, Zhejiang Ocean University, Zhoushan 316022, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China【Objective】The study aims to explore the composition, sequence number and diversity of microbial community in Zhuhai Wailingding Marine Ranch, analyze the sensitivity and reducing power of eDNA technology, with a view to providing references for the management and maintenance of ecosystem in Zhuhai Wailingding Marine Ranch.【Method】In September 2021, 9 stations were set up in Zhuhai Wailingding Marine Ranch to sample at 2 m below the surface water for pretreatment, DNA extraction, PCR amplification, and high-throughput sequencing to analyze the composition of microbial species, the density and abundance of microbial resources, and determine the dominant species.【Result】A total of 30 species of microorganisms in Zhuhai Wailingding Marine Ranch were detected by eDNA technology, and 5 phyla, 7 classes, 14 orders, 19 families and 26 genera were identified, and a total of 779 706 Operational Taxonomic Units (OTUS) were detected. The Proteobacteria was the absolute dominant phylum accounting for 75.06%, followed by Bacteroidetes (11.35%), Firmicutes (5.28%), Planctomycetes (1.91%), and Actinobacteria (1.06%). There were 7 dominant species, which were Pseudoalteromonas, Marinobacter, Moraxella, Psychrobacter, Vibrio alginolyticus, Halomonas and Sphingobacterium. The dominance of Vibrio alginolyticus reached 0.19, which was the absolute dominant species, followed by Moraxella and Pseudoalteromonas reaching 0.134 and 0.127, respectively, which were the main dominant species.【Conclusion】The study reveals the microbial community information of the Zhuhai Wailingding Marine Ranch, explores the application of eDNA technology in marine ranch, and verifies the efficiency and sensitivity of this technology, providing basic data and scientific reference for future research on marine ranching microorganisms.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202302008ednamarine ranchdiversitypcr amplificationmicrooganismdominance |
| spellingShingle | Nengwei ZANG Aiyi ZHU Pimao CHEN Huarong YUAN Honghui ZHANG Wendi WEI Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology Guangdong nongye kexue edna marine ranch diversity pcr amplification microoganism dominance |
| title | Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology |
| title_full | Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology |
| title_fullStr | Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology |
| title_full_unstemmed | Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology |
| title_short | Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology |
| title_sort | research on bacteria diversity of marine ranch in zhuhai wailingding based on edna technology |
| topic | edna marine ranch diversity pcr amplification microoganism dominance |
| url | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202302008 |
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