Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth.
<h4>Background</h4>We conducted a literature review on the studies that investigated the relationship of preterm birth, including spontaneous preterm birth (sPTB), with vitamin D status. Overall, these studies demonstrated that the incidence of sPTB was associated with maternal vitamin D...
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| _version_ | 1832592689240997888 |
|---|---|
| author | Aishwarya P Yadama Hooman Mirzakhani Thomas F McElrath Augusto A Litonjua Scott T Weiss |
| author_facet | Aishwarya P Yadama Hooman Mirzakhani Thomas F McElrath Augusto A Litonjua Scott T Weiss |
| author_sort | Aishwarya P Yadama |
| collection | DOAJ |
| description | <h4>Background</h4>We conducted a literature review on the studies that investigated the relationship of preterm birth, including spontaneous preterm birth (sPTB), with vitamin D status. Overall, these studies demonstrated that the incidence of sPTB was associated with maternal vitamin D insufficiency in early pregnancy. However, the potential mechanisms and biological pathways are unknown.<h4>Objectives</h4>To investigate early pregnancy gene expression signatures associated with both vitamin D insufficiency and sPTB. We further constructed a network of these gene signatures and identified the common biological pathways involved.<h4>Study design</h4>We conducted peripheral blood transcriptome profiling at 10-18 weeks of gestation in a nested case-control cohort of 24 pregnant women who participated in the Vitamin D Antenatal Asthma Reduction Trial (VDAART). In this cohort, 8 women had spontaneous preterm delivery (21-32 weeks of gestation) and 17 women had vitamin D insufficiency (25-hydroxyvitamin D < 30 ng/mL). We separately identified vitamin D-associated and sPTB gene signatures at 10 to 18 weeks and replicated the overlapping signatures in the mid-pregnancy peripheral blood of an independent cohort with sPTB cases.<h4>Result</h4>At 10-18 weeks of gestation, 146 differentially expressed genes (25 upregulated) were associated with both vitamin D insufficiency and sPTB in the discovery cohort (FDR < 0.05). Of these genes, 43 (25 upregulated) were replicated in the independent cohort of sPTB cases and controls with normal pregnancies (P < 0.05). Functional enrichment and network analyses of the replicated gene signatures suggested several highly connected nodes related to inflammatory and immune responses.<h4>Conclusions</h4>Our gene expression study and network analyses suggest that the dysregulation of immune response pathways due to early pregnancy vitamin D insufficiency may contribute to the pathobiology of sPTB. |
| format | Article |
| id | doaj-art-0b0618d635d346a3a2aa992c3c310c5a |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-0b0618d635d346a3a2aa992c3c310c5a2025-01-21T05:31:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01151e022719310.1371/journal.pone.0227193Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth.Aishwarya P YadamaHooman MirzakhaniThomas F McElrathAugusto A LitonjuaScott T Weiss<h4>Background</h4>We conducted a literature review on the studies that investigated the relationship of preterm birth, including spontaneous preterm birth (sPTB), with vitamin D status. Overall, these studies demonstrated that the incidence of sPTB was associated with maternal vitamin D insufficiency in early pregnancy. However, the potential mechanisms and biological pathways are unknown.<h4>Objectives</h4>To investigate early pregnancy gene expression signatures associated with both vitamin D insufficiency and sPTB. We further constructed a network of these gene signatures and identified the common biological pathways involved.<h4>Study design</h4>We conducted peripheral blood transcriptome profiling at 10-18 weeks of gestation in a nested case-control cohort of 24 pregnant women who participated in the Vitamin D Antenatal Asthma Reduction Trial (VDAART). In this cohort, 8 women had spontaneous preterm delivery (21-32 weeks of gestation) and 17 women had vitamin D insufficiency (25-hydroxyvitamin D < 30 ng/mL). We separately identified vitamin D-associated and sPTB gene signatures at 10 to 18 weeks and replicated the overlapping signatures in the mid-pregnancy peripheral blood of an independent cohort with sPTB cases.<h4>Result</h4>At 10-18 weeks of gestation, 146 differentially expressed genes (25 upregulated) were associated with both vitamin D insufficiency and sPTB in the discovery cohort (FDR < 0.05). Of these genes, 43 (25 upregulated) were replicated in the independent cohort of sPTB cases and controls with normal pregnancies (P < 0.05). Functional enrichment and network analyses of the replicated gene signatures suggested several highly connected nodes related to inflammatory and immune responses.<h4>Conclusions</h4>Our gene expression study and network analyses suggest that the dysregulation of immune response pathways due to early pregnancy vitamin D insufficiency may contribute to the pathobiology of sPTB.https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0227193/1/pone.0227193.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210223%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210223T064944Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| spellingShingle | Aishwarya P Yadama Hooman Mirzakhani Thomas F McElrath Augusto A Litonjua Scott T Weiss Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth. PLoS ONE |
| title | Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth. |
| title_full | Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth. |
| title_fullStr | Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth. |
| title_full_unstemmed | Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth. |
| title_short | Transcriptome analysis of early pregnancy vitamin D status and spontaneous preterm birth. |
| title_sort | transcriptome analysis of early pregnancy vitamin d status and spontaneous preterm birth |
| url | https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0227193/1/pone.0227193.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210223%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210223T064944Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
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