Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureus
Staphylococcus aureus is a major human pathogen that causes a great diversity of community- and hospital-acquired infections. Rsp, a member of AraC/XylS family of transcriptional regulators (AFTRs), has been reported to play an important role in the regulation of virulence determinants in S. aureus...
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1752116 |
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| author | Banghui Liu Baolin Sun |
| author_facet | Banghui Liu Baolin Sun |
| author_sort | Banghui Liu |
| collection | DOAJ |
| description | Staphylococcus aureus is a major human pathogen that causes a great diversity of community- and hospital-acquired infections. Rsp, a member of AraC/XylS family of transcriptional regulators (AFTRs), has been reported to play an important role in the regulation of virulence determinants in S. aureus via an agr-dependent pathway. Here we demonstrated that Rsp could bind to the rsp promoter to positively regulate its own expression. We then constructed an isogenic rsp deletion strain and compared the haemolysis in the wild-type and rsp mutant strains. Our results indicated that the rsp mutant strain displayed decreased haemolytic activity, which was correlated with a dramatic decrease in the expression of hla and psm. Furthermore, we analysed the regulatory effects of Rsp in the agr mutant strain and found that they are agr-independent. Electrophoretic mobility shift assay indicated that Rsp can directly bind to the promoter regions of hla and psm. The mouse model of subcutaneous abscess showed that the rsp mutant strain displayed a significant defect in virulence compared to the wild-type strain. These findings reveal that Rsp positively regulates the virulence of S. aureus by promoting the expression of hla and psm through direct binding to their promoter regions. |
| format | Article |
| id | doaj-art-0b03d41dffb84a46b3cb36d6b008b11a |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-0b03d41dffb84a46b3cb36d6b008b11a2025-08-20T03:08:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019179681210.1080/22221751.2020.1752116Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureusBanghui Liu0Baolin Sun1Department of Oncology, The First Affiliated Hospital, University of Science and Technology of China, Hefei, People’s Republic of ChinaDepartment of Oncology, The First Affiliated Hospital, University of Science and Technology of China, Hefei, People’s Republic of ChinaStaphylococcus aureus is a major human pathogen that causes a great diversity of community- and hospital-acquired infections. Rsp, a member of AraC/XylS family of transcriptional regulators (AFTRs), has been reported to play an important role in the regulation of virulence determinants in S. aureus via an agr-dependent pathway. Here we demonstrated that Rsp could bind to the rsp promoter to positively regulate its own expression. We then constructed an isogenic rsp deletion strain and compared the haemolysis in the wild-type and rsp mutant strains. Our results indicated that the rsp mutant strain displayed decreased haemolytic activity, which was correlated with a dramatic decrease in the expression of hla and psm. Furthermore, we analysed the regulatory effects of Rsp in the agr mutant strain and found that they are agr-independent. Electrophoretic mobility shift assay indicated that Rsp can directly bind to the promoter regions of hla and psm. The mouse model of subcutaneous abscess showed that the rsp mutant strain displayed a significant defect in virulence compared to the wild-type strain. These findings reveal that Rsp positively regulates the virulence of S. aureus by promoting the expression of hla and psm through direct binding to their promoter regions.https://www.tandfonline.com/doi/10.1080/22221751.2020.1752116Staphylococcus aureusRspAgrhaemolysisHlaPSMs |
| spellingShingle | Banghui Liu Baolin Sun Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureus Emerging Microbes and Infections Staphylococcus aureus Rsp Agr haemolysis Hla PSMs |
| title | Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureus |
| title_full | Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureus |
| title_fullStr | Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureus |
| title_full_unstemmed | Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureus |
| title_short | Rsp promotes the transcription of virulence factors in an agr-independent manner in Staphylococcus aureus |
| title_sort | rsp promotes the transcription of virulence factors in an agr independent manner in staphylococcus aureus |
| topic | Staphylococcus aureus Rsp Agr haemolysis Hla PSMs |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1752116 |
| work_keys_str_mv | AT banghuiliu rsppromotesthetranscriptionofvirulencefactorsinanagrindependentmannerinstaphylococcusaureus AT baolinsun rsppromotesthetranscriptionofvirulencefactorsinanagrindependentmannerinstaphylococcusaureus |