No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation Schemes
The aim of this work was to investigate the relationship between the growth rate of tumor cells and their fractionation gain. Two head and neck squamous cell carcinoma (HNSCC) cell lines, one human papillomavirus (HPV) negative (HPV−) and one HPV+, and a primary fibroblast cell line were supplemente...
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MDPI AG
2025-04-01
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| Series: | Radiation |
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| author | Lena Blanke Laura S. Hildebrand Rainer Fietkau Luitpold Distel |
| author_facet | Lena Blanke Laura S. Hildebrand Rainer Fietkau Luitpold Distel |
| author_sort | Lena Blanke |
| collection | DOAJ |
| description | The aim of this work was to investigate the relationship between the growth rate of tumor cells and their fractionation gain. Two head and neck squamous cell carcinoma (HNSCC) cell lines, one human papillomavirus (HPV) negative (HPV−) and one HPV+, and a primary fibroblast cell line were supplemented with four different concentrations of fetal bovine serum (FBS) to achieve different division rates. The effect of five different fractionation regimens was studied, namely 1 × 10 Gy, 2 × 5 Gy, 3 × 3.3 Gy, 4 × 2.5 Gy, and 5 × 2 Gy. Survival was studied using the colony-forming assay. Different concentrations of FBS were used to achieve different doubling rates for all cell lines. The HPV+ cell line was significantly more sensitive to radiation than the HPV− cell line in all fractionation schemes. The fibroblast cell line was less sensitive at low fractionation compared to the tumor cell lines. Low fractionation had a significantly higher effect, except for 5 × 2 Gy fractionation, which had a higher effect than 4 × 2.5 Gy. The number of radiosensitive mitoses during irradiation in the fractionation scheme could not explain the higher effect of 5 × 2 Gy. There was no difference in survival with the four different concentrations of FBS in all three cell lines and different fractionations. The doubling time (DT) rates of cell lines resulting from FBS deprivation do not reflect the expected increased radiation sensitivity of rapidly dividing cells. |
| format | Article |
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| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-0afa142ff69d417588fe51433eea8a402025-08-20T02:21:50ZengMDPI AGRadiation2673-592X2025-04-01521410.3390/radiation5020014No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation SchemesLena Blanke0Laura S. Hildebrand1Rainer Fietkau2Luitpold Distel3Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Universitätsstr. 27, 91054 Erlangen, GermanyDepartment of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Universitätsstr. 27, 91054 Erlangen, GermanyDepartment of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Universitätsstr. 27, 91054 Erlangen, GermanyDepartment of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Universitätsstr. 27, 91054 Erlangen, GermanyThe aim of this work was to investigate the relationship between the growth rate of tumor cells and their fractionation gain. Two head and neck squamous cell carcinoma (HNSCC) cell lines, one human papillomavirus (HPV) negative (HPV−) and one HPV+, and a primary fibroblast cell line were supplemented with four different concentrations of fetal bovine serum (FBS) to achieve different division rates. The effect of five different fractionation regimens was studied, namely 1 × 10 Gy, 2 × 5 Gy, 3 × 3.3 Gy, 4 × 2.5 Gy, and 5 × 2 Gy. Survival was studied using the colony-forming assay. Different concentrations of FBS were used to achieve different doubling rates for all cell lines. The HPV+ cell line was significantly more sensitive to radiation than the HPV− cell line in all fractionation schemes. The fibroblast cell line was less sensitive at low fractionation compared to the tumor cell lines. Low fractionation had a significantly higher effect, except for 5 × 2 Gy fractionation, which had a higher effect than 4 × 2.5 Gy. The number of radiosensitive mitoses during irradiation in the fractionation scheme could not explain the higher effect of 5 × 2 Gy. There was no difference in survival with the four different concentrations of FBS in all three cell lines and different fractionations. The doubling time (DT) rates of cell lines resulting from FBS deprivation do not reflect the expected increased radiation sensitivity of rapidly dividing cells.https://www.mdpi.com/2673-592X/5/2/14fractionation gainhead and neck squamous cell carcinomaHPV negativeHPV positiveprimary fibroblastsgrowth rate |
| spellingShingle | Lena Blanke Laura S. Hildebrand Rainer Fietkau Luitpold Distel No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation Schemes Radiation fractionation gain head and neck squamous cell carcinoma HPV negative HPV positive primary fibroblasts growth rate |
| title | No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation Schemes |
| title_full | No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation Schemes |
| title_fullStr | No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation Schemes |
| title_full_unstemmed | No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation Schemes |
| title_short | No Survival Effect in Cell Lines with Different Growth Factor-Induced Division Rates, but with Different Fractionation Schemes |
| title_sort | no survival effect in cell lines with different growth factor induced division rates but with different fractionation schemes |
| topic | fractionation gain head and neck squamous cell carcinoma HPV negative HPV positive primary fibroblasts growth rate |
| url | https://www.mdpi.com/2673-592X/5/2/14 |
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