Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn
This research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursa in Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donor materials were obtained during the 2012 winter season in the greenhous...
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| Format: | Article |
| Language: | English |
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Ankara University
2019-03-01
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| Series: | Journal of Agricultural Sciences |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/339804 |
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| author | İlhan Turgut 2. duran Zararsız 3. semra Yanıkoğlu 4. leyla Öztür Akar 5. sezgin Kızık 6. burak Bilgin |
| author_facet | İlhan Turgut 2. duran Zararsız 3. semra Yanıkoğlu 4. leyla Öztür Akar 5. sezgin Kızık 6. burak Bilgin |
| author_sort | İlhan Turgut |
| collection | DOAJ |
| description | This research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursa in Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donor materials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, provided by University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing was performed during the 2013 summer season. The haploid selection and chromosome doubling were performed during the 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3, DNR4, DNR5, DNR6, DNR7 respectively, from each donor different amount of ear crosses were performed (DNR1:16 ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to the present study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in the greenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the field, 78.3-92.6% of these plants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending on the donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR) changes from donor to donor, its mean genotype and environment is effective for HIR. Average chromosome doubling rate is lower than other researchers’ results, it is also effected by genotype and chromosome doubling methods. Zea mays; Haploid line; Double haploid line; In vivo induction |
| format | Article |
| id | doaj-art-0af74ef343e64a3b9f302f3d75fada48 |
| institution | DOAJ |
| issn | 1300-7580 2148-9297 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | Ankara University |
| record_format | Article |
| series | Journal of Agricultural Sciences |
| spelling | doaj-art-0af74ef343e64a3b9f302f3d75fada482025-08-20T03:03:54ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972019-03-01251626910.15832/ankutbd.53900045Production of Double Haploid Plants Using In Vivo Haploid Techniques in Cornİlhan Turgut2. duran Zararsız3. semra Yanıkoğlu4. leyla Öztür Akar5. sezgin Kızık6. burak BilginThis research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursa in Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donor materials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, provided by University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing was performed during the 2013 summer season. The haploid selection and chromosome doubling were performed during the 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3, DNR4, DNR5, DNR6, DNR7 respectively, from each donor different amount of ear crosses were performed (DNR1:16 ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to the present study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in the greenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the field, 78.3-92.6% of these plants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending on the donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR) changes from donor to donor, its mean genotype and environment is effective for HIR. Average chromosome doubling rate is lower than other researchers’ results, it is also effected by genotype and chromosome doubling methods. Zea mays; Haploid line; Double haploid line; In vivo inductionhttps://dergipark.org.tr/tr/download/article-file/339804zea mayshaploid linedouble haploid line |
| spellingShingle | İlhan Turgut 2. duran Zararsız 3. semra Yanıkoğlu 4. leyla Öztür Akar 5. sezgin Kızık 6. burak Bilgin Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn Journal of Agricultural Sciences zea mays haploid line double haploid line |
| title | Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn |
| title_full | Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn |
| title_fullStr | Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn |
| title_full_unstemmed | Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn |
| title_short | Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn |
| title_sort | production of double haploid plants using in vivo haploid techniques in corn |
| topic | zea mays haploid line double haploid line |
| url | https://dergipark.org.tr/tr/download/article-file/339804 |
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