Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>

The unique chaperone-like properties of C19orf53, discovered in 2020 as a “hero” protein, make it an intriguing subject for research in relation to ischemic stroke (IS). Our pilot study aimed to investigate whether C19orf53 SNPs are associated with IS. DNA samples from 2138 Russian subjects (947 IS...

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Main Authors: Irina Shilenok, Ksenia Kobzeva, Alexey Deykin, Vladimir Pokrovsky, Evgeny Patrakhanov, Olga Bushueva
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/14/9/1158
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author Irina Shilenok
Ksenia Kobzeva
Alexey Deykin
Vladimir Pokrovsky
Evgeny Patrakhanov
Olga Bushueva
author_facet Irina Shilenok
Ksenia Kobzeva
Alexey Deykin
Vladimir Pokrovsky
Evgeny Patrakhanov
Olga Bushueva
author_sort Irina Shilenok
collection DOAJ
description The unique chaperone-like properties of C19orf53, discovered in 2020 as a “hero” protein, make it an intriguing subject for research in relation to ischemic stroke (IS). Our pilot study aimed to investigate whether C19orf53 SNPs are associated with IS. DNA samples from 2138 Russian subjects (947 IS and 1308 controls) were genotyped for 7 <i>C19orf53</i> SNPs using probe-based PCR. Dominant (D), recessive (R), and log-additive (A) regression models in relation to the effect alleles (EA) were used to interpret associations. An increased risk of IS was associated with rs10104 (EA G; P<sub>bonf(R)</sub> = 0.0009; P<sub>bonf(A)</sub> = 0.0004), rs11666524 (EA A; P<sub>bonf(R)</sub> = 0.003; P<sub>bonf(A)</sub> = 0.02), rs346158 (EA C; P<sub>bonf(R)</sub> = 0.006; P<sub>bonf(A)</sub> = 0.045), and rs2277947 (EA A; P<sub>bonf(R)</sub> = 0.002; P<sub>bonf(A)</sub> = 0.01) in patients with obesity; with rs11666524 (EA A; P<sub>bonf(R)</sub> = 0.02), rs346157 (EA G; P<sub>bonf(R)</sub> = 0.036), rs346158 (EA C; P<sub>bonf(R)</sub> = 0.005), and rs2277947 (EA A; P<sub>bonf(R)</sub> = 0.02) in patients with low fruit and vegetable intake; and with rs10104 (EA G; P<sub>bonf(R)</sub> = 0.03) and rs11666524 (EA A; P<sub>bonf(R)</sub> = 0.048) in patients with low physical activity. In conclusion, our pilot study provides comprehensive genetic and bioinformatic evidence of the involvement of <i>C19orf53</i> in IS risk.
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spelling doaj-art-0aefef2aac67495e8949ea8ee6f902b32025-08-20T01:55:37ZengMDPI AGLife2075-17292024-09-01149115810.3390/life14091158Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>Irina Shilenok0Ksenia Kobzeva1Alexey Deykin2Vladimir Pokrovsky3Evgeny Patrakhanov4Olga Bushueva5Laboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaLaboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaLaboratory of Genome Editing for Biomedicine and Animal Health, Belgorod State National Research University, 308015 Belgorod, RussiaLaboratory of Genetic Technologies and Gene Editing for Biomedicine and Veterinary Medicine, Belgorod State National Research University, 308015 Belgorod, RussiaLaboratory of Genetic Technologies and Gene Editing for Biomedicine and Veterinary Medicine, Belgorod State National Research University, 308015 Belgorod, RussiaLaboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaThe unique chaperone-like properties of C19orf53, discovered in 2020 as a “hero” protein, make it an intriguing subject for research in relation to ischemic stroke (IS). Our pilot study aimed to investigate whether C19orf53 SNPs are associated with IS. DNA samples from 2138 Russian subjects (947 IS and 1308 controls) were genotyped for 7 <i>C19orf53</i> SNPs using probe-based PCR. Dominant (D), recessive (R), and log-additive (A) regression models in relation to the effect alleles (EA) were used to interpret associations. An increased risk of IS was associated with rs10104 (EA G; P<sub>bonf(R)</sub> = 0.0009; P<sub>bonf(A)</sub> = 0.0004), rs11666524 (EA A; P<sub>bonf(R)</sub> = 0.003; P<sub>bonf(A)</sub> = 0.02), rs346158 (EA C; P<sub>bonf(R)</sub> = 0.006; P<sub>bonf(A)</sub> = 0.045), and rs2277947 (EA A; P<sub>bonf(R)</sub> = 0.002; P<sub>bonf(A)</sub> = 0.01) in patients with obesity; with rs11666524 (EA A; P<sub>bonf(R)</sub> = 0.02), rs346157 (EA G; P<sub>bonf(R)</sub> = 0.036), rs346158 (EA C; P<sub>bonf(R)</sub> = 0.005), and rs2277947 (EA A; P<sub>bonf(R)</sub> = 0.02) in patients with low fruit and vegetable intake; and with rs10104 (EA G; P<sub>bonf(R)</sub> = 0.03) and rs11666524 (EA A; P<sub>bonf(R)</sub> = 0.048) in patients with low physical activity. In conclusion, our pilot study provides comprehensive genetic and bioinformatic evidence of the involvement of <i>C19orf53</i> in IS risk.https://www.mdpi.com/2075-1729/14/9/1158C19orf53chromosome 19 open reading frame 53SNPobesitylow fruit and vegetable intakelow physical activity
spellingShingle Irina Shilenok
Ksenia Kobzeva
Alexey Deykin
Vladimir Pokrovsky
Evgeny Patrakhanov
Olga Bushueva
Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>
Life
C19orf53
chromosome 19 open reading frame 53
SNP
obesity
low fruit and vegetable intake
low physical activity
title Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>
title_full Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>
title_fullStr Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>
title_full_unstemmed Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>
title_short Obesity and Environmental Risk Factors Significantly Modify the Association between Ischemic Stroke and the Hero Chaperone <i>C19orf53</i>
title_sort obesity and environmental risk factors significantly modify the association between ischemic stroke and the hero chaperone i c19orf53 i
topic C19orf53
chromosome 19 open reading frame 53
SNP
obesity
low fruit and vegetable intake
low physical activity
url https://www.mdpi.com/2075-1729/14/9/1158
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