Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plants
Banana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense, threatens global banana production. Biocontrol using Streptomyces is a promising strategy. Here, we identified Streptomyces noursei D337-11 from disease suppressive banana soils, which exhibited dual functionality in controlling F. o...
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Frontiers Media S.A.
2025-08-01
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| author | Dengbo Zhou Xinxin He Yufeng Chen Chunting Li Wei Wang Zhiqiang Pan Yankun Zhao Yongzan Wei Junting Feng Miaoyi Zhang Dengfeng Qi Xiaojuan Li Kai Li Tao Jing Jianghui Xie |
| author_facet | Dengbo Zhou Xinxin He Yufeng Chen Chunting Li Wei Wang Zhiqiang Pan Yankun Zhao Yongzan Wei Junting Feng Miaoyi Zhang Dengfeng Qi Xiaojuan Li Kai Li Tao Jing Jianghui Xie |
| author_sort | Dengbo Zhou |
| collection | DOAJ |
| description | Banana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense, threatens global banana production. Biocontrol using Streptomyces is a promising strategy. Here, we identified Streptomyces noursei D337-11 from disease suppressive banana soils, which exhibited dual functionality in controlling F. oxysporum f. sp. cubense tropical race 4 (Foc TR4), achieving a 65.35% suppression of disease, and promoting plant growth in pot experiments. The extracts from this strain restored the soil microbiota disrupted by Foc TR4, particularly, reviving Saccharimonadales populations. Using integrated untargeted metabolomics (LC-ESIMS/MS) and genomic analysis, we identified five bioactive metabolites, including naringenin, a flavonoid linked to a 100% homologous biosynthetic gene cluster (Region 52.1). Genome-guided differential metabolomics established the genetic basis for flavonoid production. Mechanistic characterization indicated that naringenin exhibits antifungal activity via dual-target inhibition: molecular docking showed strong binding to the trypsin of Foc TR4 (N1RZA7, −6.6 kcal/mol) and nitroalkane oxidase (N1R9V2, −8.4 kcal/mol), which disrupts cellular integrity as evidenced by ultrastructural anomalies. We provide the first evidence of Streptomyces-derived naringenin as a multi-target antifungal agent. Overall, this study provides a theoretical basis for exploring the application of microbial flavonoids in biological control of fungal diseases. |
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| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-0aedef22b52d4f4184142e4a5920806f2025-08-26T05:28:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.16551031655103Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plantsDengbo Zhou0Xinxin He1Yufeng Chen2Chunting Li3Wei Wang4Zhiqiang Pan5Yankun Zhao6Yongzan Wei7Junting Feng8Miaoyi Zhang9Dengfeng Qi10Xiaojuan Li11Kai Li12Tao Jing13Jianghui Xie14National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNatural Products Utilization Research Unit, Agricultural Research Service, U.S. Department of Agriculture, University of Mississippi, Oxford, MS, United StatesNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, ChinaBanana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense, threatens global banana production. Biocontrol using Streptomyces is a promising strategy. Here, we identified Streptomyces noursei D337-11 from disease suppressive banana soils, which exhibited dual functionality in controlling F. oxysporum f. sp. cubense tropical race 4 (Foc TR4), achieving a 65.35% suppression of disease, and promoting plant growth in pot experiments. The extracts from this strain restored the soil microbiota disrupted by Foc TR4, particularly, reviving Saccharimonadales populations. Using integrated untargeted metabolomics (LC-ESIMS/MS) and genomic analysis, we identified five bioactive metabolites, including naringenin, a flavonoid linked to a 100% homologous biosynthetic gene cluster (Region 52.1). Genome-guided differential metabolomics established the genetic basis for flavonoid production. Mechanistic characterization indicated that naringenin exhibits antifungal activity via dual-target inhibition: molecular docking showed strong binding to the trypsin of Foc TR4 (N1RZA7, −6.6 kcal/mol) and nitroalkane oxidase (N1R9V2, −8.4 kcal/mol), which disrupts cellular integrity as evidenced by ultrastructural anomalies. We provide the first evidence of Streptomyces-derived naringenin as a multi-target antifungal agent. Overall, this study provides a theoretical basis for exploring the application of microbial flavonoids in biological control of fungal diseases.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1655103/fullStreptomyces nourseigenome-guided differential metabolomicsbanana fusarium wiltnaringenintarget protein |
| spellingShingle | Dengbo Zhou Xinxin He Yufeng Chen Chunting Li Wei Wang Zhiqiang Pan Yankun Zhao Yongzan Wei Junting Feng Miaoyi Zhang Dengfeng Qi Xiaojuan Li Kai Li Tao Jing Jianghui Xie Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plants Frontiers in Microbiology Streptomyces noursei genome-guided differential metabolomics banana fusarium wilt naringenin target protein |
| title | Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plants |
| title_full | Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plants |
| title_fullStr | Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plants |
| title_full_unstemmed | Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plants |
| title_short | Biocontrol potential of newly isolated Streptomyces noursei D337-11 from disease suppressive soil and its metabolites against Fusarium oxysporum f. sp. cubense in banana plants |
| title_sort | biocontrol potential of newly isolated streptomyces noursei d337 11 from disease suppressive soil and its metabolites against fusarium oxysporum f sp cubense in banana plants |
| topic | Streptomyces noursei genome-guided differential metabolomics banana fusarium wilt naringenin target protein |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1655103/full |
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