Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing
Abstract The complex and dynamic nature of aquatic ecosystems, particularly in marine environments, makes managing wound infections a significant challenge for individuals engaged in underwater activities and for aquatic organisms. Although antibiotics have played a critical role in safeguarding hum...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-04-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202415169 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849767185014063104 |
|---|---|
| author | Qingsong Zhang Menghan Lu Richang Ou Hong Lin Guanhua Xuan Xiudan Wang Xiaofeng Xu Weiwei Zhang Guoqing Wang |
| author_facet | Qingsong Zhang Menghan Lu Richang Ou Hong Lin Guanhua Xuan Xiudan Wang Xiaofeng Xu Weiwei Zhang Guoqing Wang |
| author_sort | Qingsong Zhang |
| collection | DOAJ |
| description | Abstract The complex and dynamic nature of aquatic ecosystems, particularly in marine environments, makes managing wound infections a significant challenge for individuals engaged in underwater activities and for aquatic organisms. Although antibiotics have played a critical role in safeguarding humans and aquatic health, their risk of drug resistance and environmental impact present substantial obstacles to long‐term sustainability. Using fin rot disease in turbot (Scophthalmus maximus) caused by infection of Vibrio anguillarum (V. anguillarum) as a model, a new strategy is presented that employs a carbon dot (CD)‐based antisense oligonucleotide (ASO) delivery system, combined with an adhesive hydrogel, to achieve targeted gene silencing of V. anguillarum for underwater healing. The CDs that cause enhanced cytoplasmic membrane permeability, efficiently deliver ASOs into V. anguillarum without requiring additional equipment or chemical facilitators. The specific design of the ASO sequence enables targeted silencing of empA, achieving efficiency as high as 71.2%. An adhesive hydrogel is applied to boost the local concentration of ASO/CDs at wound sites in seawater, effectively sealing the infected area and preventing fin rot disease in turbot. This study pioneer targeted bacterial gene regulation using CD‐based delivery integrated with a hydrogel bandage, offering practical solutions for managing underwater bacterial diseases. |
| format | Article |
| id | doaj-art-0aedb4f0149e42ea876c9833cb5fcd05 |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-0aedb4f0149e42ea876c9833cb5fcd052025-08-20T03:04:17ZengWileyAdvanced Science2198-38442025-04-011213n/an/a10.1002/advs.202415169Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound HealingQingsong Zhang0Menghan Lu1Richang Ou2Hong Lin3Guanhua Xuan4Xiudan Wang5Xiaofeng Xu6Weiwei Zhang7Guoqing Wang8MOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity Ocean University of China 5 Yushan Road Qingdao 266003 ChinaMOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity Ocean University of China 5 Yushan Road Qingdao 266003 ChinaCollege of Materials Science and Engineering Ocean University of China Qingdao 266100 ChinaSKL of Marine Food Processing & Safety Control College of Food Science and Engineering Ocean University of China 1299 Sansha Road Qingdao 266404 ChinaSKL of Marine Food Processing & Safety Control College of Food Science and Engineering Ocean University of China 1299 Sansha Road Qingdao 266404 ChinaSKL of Marine Food Processing & Safety Control College of Food Science and Engineering Ocean University of China 1299 Sansha Road Qingdao 266404 ChinaCollege of Materials Science and Engineering Ocean University of China Qingdao 266100 ChinaSchool of Marine Sciences Ningbo University 169 Qixingnan Road Ningbo 315832 ChinaMOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity Ocean University of China 5 Yushan Road Qingdao 266003 ChinaAbstract The complex and dynamic nature of aquatic ecosystems, particularly in marine environments, makes managing wound infections a significant challenge for individuals engaged in underwater activities and for aquatic organisms. Although antibiotics have played a critical role in safeguarding humans and aquatic health, their risk of drug resistance and environmental impact present substantial obstacles to long‐term sustainability. Using fin rot disease in turbot (Scophthalmus maximus) caused by infection of Vibrio anguillarum (V. anguillarum) as a model, a new strategy is presented that employs a carbon dot (CD)‐based antisense oligonucleotide (ASO) delivery system, combined with an adhesive hydrogel, to achieve targeted gene silencing of V. anguillarum for underwater healing. The CDs that cause enhanced cytoplasmic membrane permeability, efficiently deliver ASOs into V. anguillarum without requiring additional equipment or chemical facilitators. The specific design of the ASO sequence enables targeted silencing of empA, achieving efficiency as high as 71.2%. An adhesive hydrogel is applied to boost the local concentration of ASO/CDs at wound sites in seawater, effectively sealing the infected area and preventing fin rot disease in turbot. This study pioneer targeted bacterial gene regulation using CD‐based delivery integrated with a hydrogel bandage, offering practical solutions for managing underwater bacterial diseases.https://doi.org/10.1002/advs.202415169antisense oligonucleotidecarbon dotgene regulationnucleic acid deliveryunderwater wound treatment |
| spellingShingle | Qingsong Zhang Menghan Lu Richang Ou Hong Lin Guanhua Xuan Xiudan Wang Xiaofeng Xu Weiwei Zhang Guoqing Wang Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing Advanced Science antisense oligonucleotide carbon dot gene regulation nucleic acid delivery underwater wound treatment |
| title | Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing |
| title_full | Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing |
| title_fullStr | Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing |
| title_full_unstemmed | Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing |
| title_short | Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing |
| title_sort | nanodot inspired precise bacterial gene suppression in a smart hydrogel bandage for underwater wound healing |
| topic | antisense oligonucleotide carbon dot gene regulation nucleic acid delivery underwater wound treatment |
| url | https://doi.org/10.1002/advs.202415169 |
| work_keys_str_mv | AT qingsongzhang nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT menghanlu nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT richangou nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT honglin nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT guanhuaxuan nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT xiudanwang nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT xiaofengxu nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT weiweizhang nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing AT guoqingwang nanodotinspiredprecisebacterialgenesuppressioninasmarthydrogelbandageforunderwaterwoundhealing |