Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I>
Abstract One of the diseases that often attack are fish is parasitic disease caused by Lernaea cyprinacea, called Lernaeosis. This ectoparasites could be detected on skin, gill, eyes, fin or inside of mouth and nostril of fishes. The aim of the study was to know the character is tic of L.cyprinace...
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Faculty of Fisheries and Marine Universitas Airlangga
2010-04-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/11669 |
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| author | Gunanti Mahasri Ulia Fajriah Sri Subekti |
| author_facet | Gunanti Mahasri Ulia Fajriah Sri Subekti |
| author_sort | Gunanti Mahasri |
| collection | DOAJ |
| description | Abstract
One of the diseases that often attack are fish is parasitic disease caused by Lernaea cyprinacea, called Lernaeosis. This ectoparasites could be detected on skin, gill, eyes, fin or inside of mouth and nostril of fishes. The aim of the study was to know the character is tic of L.cyprinacea protein based on molecular weight using SDS-PAGE. The results of this study were expected as Lernaeosis vaccine candidate. The experiment used SDS-PAGE with 12% separating gel, 3% stacking gel, voltage 110 volts 28 A and gel staining using coomassie blue. The result showed that, there were eight protein bands with molecular weight 82.3, 73.3, 66.6, 60.5, 54.9, 27.5, 23.1 and 19.8 kDa. Among them, the bands with molecular weight 27.5, 23.1 and 19.8 kDa were thicker and clearer than the others. |
| format | Article |
| id | doaj-art-0aea7984519944868a2caa813d3341f7 |
| institution | DOAJ |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2010-04-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-0aea7984519944868a2caa813d3341f72025-08-20T02:58:07ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592010-04-0121616610.20473/jipk.v2i1.116695923Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I>Gunanti Mahasri0Ulia Fajriah1Sri Subekti2Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.Program Studi Akuakultur, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.Abstract One of the diseases that often attack are fish is parasitic disease caused by Lernaea cyprinacea, called Lernaeosis. This ectoparasites could be detected on skin, gill, eyes, fin or inside of mouth and nostril of fishes. The aim of the study was to know the character is tic of L.cyprinacea protein based on molecular weight using SDS-PAGE. The results of this study were expected as Lernaeosis vaccine candidate. The experiment used SDS-PAGE with 12% separating gel, 3% stacking gel, voltage 110 volts 28 A and gel staining using coomassie blue. The result showed that, there were eight protein bands with molecular weight 82.3, 73.3, 66.6, 60.5, 54.9, 27.5, 23.1 and 19.8 kDa. Among them, the bands with molecular weight 27.5, 23.1 and 19.8 kDa were thicker and clearer than the others.https://e-journal.unair.ac.id/JIPK/article/view/11669lernaeosis, sds-page, protein |
| spellingShingle | Gunanti Mahasri Ulia Fajriah Sri Subekti Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I> Jurnal Ilmiah Perikanan dan Kelautan lernaeosis, sds-page, protein |
| title | Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I> |
| title_full | Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I> |
| title_fullStr | Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I> |
| title_full_unstemmed | Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I> |
| title_short | Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I> |
| title_sort | karakterisasi protein lernaea cyprinacea dengan metode elektroforesis sds page br i characterization of protein lernaea cyprinacea by using sds page electrophoresis method i |
| topic | lernaeosis, sds-page, protein |
| url | https://e-journal.unair.ac.id/JIPK/article/view/11669 |
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