Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies
Introduction. Laser-induced thermal effects can preclude a safe histological evaluation of biopsy resection margins. The aim of this study was to evaluate the suitability of a 976 nm diode laser in oral soft tissue biopsies in an ex vivo study. Materials and Methods. A 976 nm diode laser (Solase®, L...
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2021-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2021/6658268 |
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| author | Gaspare Palaia Federico Renzi Daniele Pergolini Alessandro Del Vecchio Paolo Visca Gianluca Tenore Umberto Romeo |
| author_facet | Gaspare Palaia Federico Renzi Daniele Pergolini Alessandro Del Vecchio Paolo Visca Gianluca Tenore Umberto Romeo |
| author_sort | Gaspare Palaia |
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| description | Introduction. Laser-induced thermal effects can preclude a safe histological evaluation of biopsy resection margins. The aim of this study was to evaluate the suitability of a 976 nm diode laser in oral soft tissue biopsies in an ex vivo study. Materials and Methods. A 976 nm diode laser (Solase®, Lazon Medical Laser, China) has been used in the contact mode, using a 400 μm fiber tip, at different parameters from 4 to 6 W in the continuous wave (CW), with a fluence between 3184 and 4777 J/cm2, and pulsed wave (PW) mode, with a fluence between 318,4 and 477,7 J/cm2, to obtain 30 samples from fresh pig cadaver tongues. All specimens were subdivided into 6 groups (from A to F), and each group consisted of 5 samples. Two sections were obtained from each sample. A histological analysis was performed using an optical microscope at magnifications of 5x and 10x. Statistical analysis was carried out using Kruskal–Wallis and Dunn’s tests. Results. The results showed that histological readability was optimal in all the samples. The thermal damage was negligible in all groups. The average thermal damage was 208.40 ± 133.81 μm in the epithelial tissue and 330.14 ± 147.45 μm in the connective tissue. The statistical analysis showed no differences between the groups (p > 0.05). Conclusion. A 976 nm diode laser demonstrated good surgical effectiveness that provoked little peripheral damage in the cut edges and allowed a safe histological diagnosis. Clinical Relevance. In oral pathology, many times, there is fear in using the laser to remove some lesions due to its thermal effect on the tissues close to the lesion. This effect is always present in the use of the laser, but the intent is to minimize this effect to have as little alteration as possible on the surrounding tissues. |
| format | Article |
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| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | International Journal of Dentistry |
| spelling | doaj-art-0ae6636575b5467e9eb7ab3c6042ad5b2025-02-03T05:49:15ZengWileyInternational Journal of Dentistry1687-87281687-87362021-01-01202110.1155/2021/66582686658268Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue BiopsiesGaspare Palaia0Federico Renzi1Daniele Pergolini2Alessandro Del Vecchio3Paolo Visca4Gianluca Tenore5Umberto Romeo6“Sapienza” University of Rome, Department of Oral and Maxillofacial Sciences, Via Caserta 6, Rome 00161, Italy“Sapienza” University of Rome, Department of Oral and Maxillofacial Sciences, Via Caserta 6, Rome 00161, Italy“Sapienza” University of Rome, Department of Oral and Maxillofacial Sciences, Via Caserta 6, Rome 00161, Italy“Sapienza” University of Rome, Department of Oral and Maxillofacial Sciences, Via Caserta 6, Rome 00161, ItalyDepartment of Cytology and Cellular Diagnostics, Regina Elena Institute, Via Elio Chianesi 53, Rome 00144, Italy“Sapienza” University of Rome, Department of Oral and Maxillofacial Sciences, Via Caserta 6, Rome 00161, Italy“Sapienza” University of Rome, Department of Oral and Maxillofacial Sciences, Via Caserta 6, Rome 00161, ItalyIntroduction. Laser-induced thermal effects can preclude a safe histological evaluation of biopsy resection margins. The aim of this study was to evaluate the suitability of a 976 nm diode laser in oral soft tissue biopsies in an ex vivo study. Materials and Methods. A 976 nm diode laser (Solase®, Lazon Medical Laser, China) has been used in the contact mode, using a 400 μm fiber tip, at different parameters from 4 to 6 W in the continuous wave (CW), with a fluence between 3184 and 4777 J/cm2, and pulsed wave (PW) mode, with a fluence between 318,4 and 477,7 J/cm2, to obtain 30 samples from fresh pig cadaver tongues. All specimens were subdivided into 6 groups (from A to F), and each group consisted of 5 samples. Two sections were obtained from each sample. A histological analysis was performed using an optical microscope at magnifications of 5x and 10x. Statistical analysis was carried out using Kruskal–Wallis and Dunn’s tests. Results. The results showed that histological readability was optimal in all the samples. The thermal damage was negligible in all groups. The average thermal damage was 208.40 ± 133.81 μm in the epithelial tissue and 330.14 ± 147.45 μm in the connective tissue. The statistical analysis showed no differences between the groups (p > 0.05). Conclusion. A 976 nm diode laser demonstrated good surgical effectiveness that provoked little peripheral damage in the cut edges and allowed a safe histological diagnosis. Clinical Relevance. In oral pathology, many times, there is fear in using the laser to remove some lesions due to its thermal effect on the tissues close to the lesion. This effect is always present in the use of the laser, but the intent is to minimize this effect to have as little alteration as possible on the surrounding tissues.http://dx.doi.org/10.1155/2021/6658268 |
| spellingShingle | Gaspare Palaia Federico Renzi Daniele Pergolini Alessandro Del Vecchio Paolo Visca Gianluca Tenore Umberto Romeo Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies International Journal of Dentistry |
| title | Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies |
| title_full | Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies |
| title_fullStr | Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies |
| title_full_unstemmed | Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies |
| title_short | Histological Ex Vivo Evaluation of the Suitability of a 976 nm Diode Laser in Oral Soft Tissue Biopsies |
| title_sort | histological ex vivo evaluation of the suitability of a 976 nm diode laser in oral soft tissue biopsies |
| url | http://dx.doi.org/10.1155/2021/6658268 |
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