Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion
Background Platinum is a commonly used drug for ovarian cancer (OvCa) treatment, but drug resistance limits its clinical application. This study intended to delineate the effects of adipocytes on platinum resistance in OvCa.Methods OvCa cells were maintained in the adipocyte-conditioned medium. Cell...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Adipocyte |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2023.2282566 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850137960848031744 |
|---|---|
| author | Jiong Ma Junyan Li Xuejun Chen Yanyan Ma |
| author_facet | Jiong Ma Junyan Li Xuejun Chen Yanyan Ma |
| author_sort | Jiong Ma |
| collection | DOAJ |
| description | Background Platinum is a commonly used drug for ovarian cancer (OvCa) treatment, but drug resistance limits its clinical application. This study intended to delineate the effects of adipocytes on platinum resistance in OvCa.Methods OvCa cells were maintained in the adipocyte-conditioned medium. Cell viability and apoptosis were detected by CCK-8 and flow cytometry, separately. Proliferation and apoptosis-related protein expression were assayed by western blot. The IC50 values of cisplatin and carboplatin were determined using CCK-8. IGF1 secretion and expression were assayed via ELISA and western blot, respectively. A xenograft model was established, and pathological changes were detected by H&E staining. Proliferation and apoptosis-associated protein expression was assessed via IHC.Results Adipocytes promoted the viability and repressed cell apoptosis in OvCa, as well as enhancing platinum resistance, while the addition of IGF-1 R inhibitor reversed the effects of adipocytes on proliferation, apoptosis, and drug resistance of OvCa cells. Treatment with different concentrations of Ojeok-san (OJS) inhibited the adipocyte-induced platinum resistance in OvCa cells by suppressing IGF1. The combined treatment of OJS and cisplatin significantly inhibited tumour growth in vivo with good mouse tolerance.Conclusion In summary, OJS inhibited OvCa proliferation and platinum resistance by suppressing adipocyte paracrine IGF1 secretion. |
| format | Article |
| id | doaj-art-0ae3bd5423bc406aa477b6c5a8567e95 |
| institution | OA Journals |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-0ae3bd5423bc406aa477b6c5a8567e952025-08-20T02:30:41ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2024-12-0113110.1080/21623945.2023.2282566Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretionJiong Ma0Junyan Li1Xuejun Chen2Yanyan Ma3Department of Gynecology, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, ChinaDepartment of Gynecology, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, ChinaDepartment of Gynecology, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, ChinaDepartment of Gynecology, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, ChinaBackground Platinum is a commonly used drug for ovarian cancer (OvCa) treatment, but drug resistance limits its clinical application. This study intended to delineate the effects of adipocytes on platinum resistance in OvCa.Methods OvCa cells were maintained in the adipocyte-conditioned medium. Cell viability and apoptosis were detected by CCK-8 and flow cytometry, separately. Proliferation and apoptosis-related protein expression were assayed by western blot. The IC50 values of cisplatin and carboplatin were determined using CCK-8. IGF1 secretion and expression were assayed via ELISA and western blot, respectively. A xenograft model was established, and pathological changes were detected by H&E staining. Proliferation and apoptosis-associated protein expression was assessed via IHC.Results Adipocytes promoted the viability and repressed cell apoptosis in OvCa, as well as enhancing platinum resistance, while the addition of IGF-1 R inhibitor reversed the effects of adipocytes on proliferation, apoptosis, and drug resistance of OvCa cells. Treatment with different concentrations of Ojeok-san (OJS) inhibited the adipocyte-induced platinum resistance in OvCa cells by suppressing IGF1. The combined treatment of OJS and cisplatin significantly inhibited tumour growth in vivo with good mouse tolerance.Conclusion In summary, OJS inhibited OvCa proliferation and platinum resistance by suppressing adipocyte paracrine IGF1 secretion.https://www.tandfonline.com/doi/10.1080/21623945.2023.2282566Ovarian canceradipocyteIGF1platinumOJS |
| spellingShingle | Jiong Ma Junyan Li Xuejun Chen Yanyan Ma Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion Adipocyte Ovarian cancer adipocyte IGF1 platinum OJS |
| title | Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion |
| title_full | Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion |
| title_fullStr | Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion |
| title_full_unstemmed | Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion |
| title_short | Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion |
| title_sort | ojeok san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine igf1 secretion |
| topic | Ovarian cancer adipocyte IGF1 platinum OJS |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2023.2282566 |
| work_keys_str_mv | AT jiongma ojeoksanenhancesplatinumsensitivityinovariancancerbyregulatingadipocyteparacrineigf1secretion AT junyanli ojeoksanenhancesplatinumsensitivityinovariancancerbyregulatingadipocyteparacrineigf1secretion AT xuejunchen ojeoksanenhancesplatinumsensitivityinovariancancerbyregulatingadipocyteparacrineigf1secretion AT yanyanma ojeoksanenhancesplatinumsensitivityinovariancancerbyregulatingadipocyteparacrineigf1secretion |