SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN
Demand for milk has increased in Malaysia due to the increased in awareness of healthy foods consumption. Hence, research of milk is crucial to ensure that it is not contaminated with Escherichia coli. This study evaluated the survival of Escherichia coliat different temperature and haemolysinact...
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| Format: | Article |
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Universiti Teknologi MARA, Negeri Sembilan
2025-04-01
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| Series: | Journal of Academia |
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| Online Access: | https://journal.uitm.edu.my/ojs/index.php/JOA/article/view/5207/3172 |
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| author | Farra Amira Mohamed Aimi Nadia Ramli Noorlis Ahmad |
| author_facet | Farra Amira Mohamed Aimi Nadia Ramli Noorlis Ahmad |
| author_sort | Farra Amira Mohamed |
| collection | DOAJ |
| description | Demand for milk has increased in Malaysia due to the increased in awareness of healthy foods consumption. Hence, research of milk is crucial to ensure that it is not contaminated with Escherichia coli. This study evaluated the survival of Escherichia coliat different temperature and haemolysinactivity of Escherichia colion blood agar. A total of 8 samples of raw fresh and pasteurized milk were collected from nearby farm and market in Negeri Sembilan, Malaysia. After an overnight exposure to four different temperatures of 00C, 280C, 350C and 450C, the bacteriological test of milk was evaluated for the presence of Escherichia coli. Overall, all raw fresh milk sampled exceeded the acceptable limit of bacterial count of 1 x 105CFU/ml. Raw fresh milk recorded the highest count at 35oC with 4.4 x 107CFU/ml and the lowest at 0oC with 8.3 x 104CFU/ml. The presence of Escherichia coliwas detected in 7/20(35%) of the total raw fresh milk samples. All pasteurized milk showed no presence of Escherichia colidue to the effectiveness of heat treatment. Haemolysin test showed no haemolytic activity. Milk contaminated with Escherichia colican cause diarrheal, gastrointestinal diseases and urinary infection. Hence, it is important to study the survival rate of Escherichia coliand its pathogenicity in milk to ensure public safety. |
| format | Article |
| id | doaj-art-0ad22f73634c48eb81d1a23f792943bd |
| institution | Kabale University |
| issn | 2289-6368 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Universiti Teknologi MARA, Negeri Sembilan |
| record_format | Article |
| series | Journal of Academia |
| spelling | doaj-art-0ad22f73634c48eb81d1a23f792943bd2025-08-20T03:49:17ZengUniversiti Teknologi MARA, Negeri SembilanJournal of Academia2289-63682025-04-0113110411510.24191/joa.v13i1.5207SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILANFarra Amira Mohamed0Aimi Nadia Ramli1Noorlis Ahmad2Faculty of Applied Sciences, Universiti Teknologi MARA,Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000, Kuala Pilah, MalaysiaFaculty of Applied Sciences, Universiti Teknologi MARA,Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000, Kuala Pilah, MalaysiaFaculty of Applied Sciences, Universiti Teknologi MARA,Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000, Kuala Pilah, MalaysiaDemand for milk has increased in Malaysia due to the increased in awareness of healthy foods consumption. Hence, research of milk is crucial to ensure that it is not contaminated with Escherichia coli. This study evaluated the survival of Escherichia coliat different temperature and haemolysinactivity of Escherichia colion blood agar. A total of 8 samples of raw fresh and pasteurized milk were collected from nearby farm and market in Negeri Sembilan, Malaysia. After an overnight exposure to four different temperatures of 00C, 280C, 350C and 450C, the bacteriological test of milk was evaluated for the presence of Escherichia coli. Overall, all raw fresh milk sampled exceeded the acceptable limit of bacterial count of 1 x 105CFU/ml. Raw fresh milk recorded the highest count at 35oC with 4.4 x 107CFU/ml and the lowest at 0oC with 8.3 x 104CFU/ml. The presence of Escherichia coliwas detected in 7/20(35%) of the total raw fresh milk samples. All pasteurized milk showed no presence of Escherichia colidue to the effectiveness of heat treatment. Haemolysin test showed no haemolytic activity. Milk contaminated with Escherichia colican cause diarrheal, gastrointestinal diseases and urinary infection. Hence, it is important to study the survival rate of Escherichia coliand its pathogenicity in milk to ensure public safety.https://journal.uitm.edu.my/ojs/index.php/JOA/article/view/5207/3172raw milksurvivalhaemolysinescherichia colitemperaturetotal plate count |
| spellingShingle | Farra Amira Mohamed Aimi Nadia Ramli Noorlis Ahmad SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN Journal of Academia raw milk survival haemolysin escherichia coli temperature total plate count |
| title | SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN |
| title_full | SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN |
| title_fullStr | SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN |
| title_full_unstemmed | SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN |
| title_short | SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN |
| title_sort | survival study and haemolysin activity of escherichia coli in raw and pasteurized milk produced in negeri sembilan |
| topic | raw milk survival haemolysin escherichia coli temperature total plate count |
| url | https://journal.uitm.edu.my/ojs/index.php/JOA/article/view/5207/3172 |
| work_keys_str_mv | AT farraamiramohamed survivalstudyandhaemolysinactivityofescherichiacoliinrawandpasteurizedmilkproducedinnegerisembilan AT aiminadiaramli survivalstudyandhaemolysinactivityofescherichiacoliinrawandpasteurizedmilkproducedinnegerisembilan AT noorlisahmad survivalstudyandhaemolysinactivityofescherichiacoliinrawandpasteurizedmilkproducedinnegerisembilan |