High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB Biomarker
Tuberculosis (TB) continues to be a significant contributor to global death tolls among infectious diseases. It can be diagnosed using certain cytokines, such as interferon‐gamma (IFN‐γ). Interferon‐gamma is an essential component of the immune system's defense against pathogens which is known...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-07-01
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| Series: | ChemElectroChem |
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| Online Access: | https://doi.org/10.1002/celc.202400654 |
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| author | Kaylin Cleo Januarie Usisipho Feleni Candice Cupido Nelia Abraham Sanga Rachel Fanelwa Ajayi Emmanuel Iheanyichukwu Iwuoha Marlon Oranzie Jaymi Leigh January Zandile Dennis Leve |
| author_facet | Kaylin Cleo Januarie Usisipho Feleni Candice Cupido Nelia Abraham Sanga Rachel Fanelwa Ajayi Emmanuel Iheanyichukwu Iwuoha Marlon Oranzie Jaymi Leigh January Zandile Dennis Leve |
| author_sort | Kaylin Cleo Januarie |
| collection | DOAJ |
| description | Tuberculosis (TB) continues to be a significant contributor to global death tolls among infectious diseases. It can be diagnosed using certain cytokines, such as interferon‐gamma (IFN‐γ). Interferon‐gamma is an essential component of the immune system's defense against pathogens which is known to be expressed in TB infection. This work involves the development of a quantum dot‐sensitized electrochemical aptasensor for IFN‐γ. The sensor system consists of gold disc electrode surface that was modified with novel L‐cysteine‐functionalized indium telluriselenide quantum dots (L‐cysteine‐InTeSe QD). Subsequently, the L‐cysteine‐InTeSe QDs are conjugated to an amine‐terminated IFN‐γ aptamer through a carbodiimide‐mediated amide bond formation. The electrochemical responses of the IFN‐γ aptasensor are studied by voltammetry. A linear calibration curve is generated for the concentration range studied (i.e., 10–21 pg mL−1 IFN‐γ), and a limit of detection of 0.312 pg mL−1 is obtained. The QD aptasensor is able to accurately detect IFN‐γ without interference from other substances. In a practical application using synthetic human serum, the QD aptasensor produced good detection recovery of 98–102%, highlighting its potential for diagnosing infectious diseases. |
| format | Article |
| id | doaj-art-0acbd8ff03374d4ea36f22064f6e4784 |
| institution | Kabale University |
| issn | 2196-0216 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemElectroChem |
| spelling | doaj-art-0acbd8ff03374d4ea36f22064f6e47842025-08-20T03:56:46ZengWiley-VCHChemElectroChem2196-02162025-07-011215n/an/a10.1002/celc.202400654High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB BiomarkerKaylin Cleo Januarie0Usisipho Feleni1Candice Cupido2Nelia Abraham Sanga3Rachel Fanelwa Ajayi4Emmanuel Iheanyichukwu Iwuoha5Marlon Oranzie6Jaymi Leigh January7Zandile Dennis Leve8SensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaInstitute for Nanotechnology and Water Sustainability (iNanoWS) College of Science, Engineering and Technology University of South Africa Florida Campus, Florida Park Johannesburg 1710 South AfricaSensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaSensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaSensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaSensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaSensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaSensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaSensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Robert Sobukwe Road Cape Town Bellville 7535 South AfricaTuberculosis (TB) continues to be a significant contributor to global death tolls among infectious diseases. It can be diagnosed using certain cytokines, such as interferon‐gamma (IFN‐γ). Interferon‐gamma is an essential component of the immune system's defense against pathogens which is known to be expressed in TB infection. This work involves the development of a quantum dot‐sensitized electrochemical aptasensor for IFN‐γ. The sensor system consists of gold disc electrode surface that was modified with novel L‐cysteine‐functionalized indium telluriselenide quantum dots (L‐cysteine‐InTeSe QD). Subsequently, the L‐cysteine‐InTeSe QDs are conjugated to an amine‐terminated IFN‐γ aptamer through a carbodiimide‐mediated amide bond formation. The electrochemical responses of the IFN‐γ aptasensor are studied by voltammetry. A linear calibration curve is generated for the concentration range studied (i.e., 10–21 pg mL−1 IFN‐γ), and a limit of detection of 0.312 pg mL−1 is obtained. The QD aptasensor is able to accurately detect IFN‐γ without interference from other substances. In a practical application using synthetic human serum, the QD aptasensor produced good detection recovery of 98–102%, highlighting its potential for diagnosing infectious diseases.https://doi.org/10.1002/celc.202400654aptasensorscytokineselectrochemical detectionsinterferon‐gammaquantum dotstuberculosis |
| spellingShingle | Kaylin Cleo Januarie Usisipho Feleni Candice Cupido Nelia Abraham Sanga Rachel Fanelwa Ajayi Emmanuel Iheanyichukwu Iwuoha Marlon Oranzie Jaymi Leigh January Zandile Dennis Leve High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB Biomarker ChemElectroChem aptasensors cytokines electrochemical detections interferon‐gamma quantum dots tuberculosis |
| title | High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB Biomarker |
| title_full | High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB Biomarker |
| title_fullStr | High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB Biomarker |
| title_full_unstemmed | High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB Biomarker |
| title_short | High Sensitivity L‐Cysteine Indium Telluriselenide Quantum Dot‐Inspired Aptasensor for Interferon Gamma TB Biomarker |
| title_sort | high sensitivity l cysteine indium telluriselenide quantum dot inspired aptasensor for interferon gamma tb biomarker |
| topic | aptasensors cytokines electrochemical detections interferon‐gamma quantum dots tuberculosis |
| url | https://doi.org/10.1002/celc.202400654 |
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