Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchids
Grammatophyllum stapeliiflorum, recognized as the melancholic orchid, represents one of the elusive orchid species facing the brink of extinction, thus exacerbating its rarity. Tissue culture technology becomes imperative for the propagation of this orchid species. This study aims to scrutinize the...
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Universitas Lancang Kuning
2024-03-01
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| Series: | Jurnal Ilmiah Pertanian |
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| Online Access: | https://dev-journal.unilak.ac.id/index.php/jip/article/view/12829 |
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| author | Yuniati Mayta Novaliza Isda |
| author_facet | Yuniati Mayta Novaliza Isda |
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| collection | DOAJ |
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Grammatophyllum stapeliiflorum, recognized as the melancholic orchid, represents one of the elusive orchid species facing the brink of extinction, thus exacerbating its rarity. Tissue culture technology becomes imperative for the propagation of this orchid species. This study aims to scrutinize the influence of different concentrations of the synthetic growth regulator thidiazuron (TDZ) on distinct basal media types, Murashige Skoog (MS) and Vacin and Went (VW), on the in vitro growth of G. stapeliiflorum orchids. Employing a complete randomized factorial design, the study entails two research factors: TDZ concentration with 4 treatment levels, A0 = 0 mg/L, A1 = 0.25 mg/L, A2 = 0.50 mg/L, and A3 = 0.75 mg/L, and the second factor being the media type, B1 = ½ MS and B2 = ½ VW. Findings indicate significant effects of MS and VW media on the percentage of viable explants, browning, globular forms, and shoots. Interaction between TDZ concentration and media type didn't yield significant effects on each experimental parameter. Treatment with 0.25 and 0.50 mg/L TDZ on ½ VW media demonstrated optimal results for explant growth percentage, browning, and explants in the globular phase, at 97.33%, 2.67%, and 2.67%, respectively. Moreover, treatment with 0.75 mg/L TDZ on ½ VW media resulted in the highest percentage of explant coloration. The combination of TDZ and media has shown the potential to enhance the production efficiency and conservation of this rare orchid species through tissue culture technology.
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| format | Article |
| id | doaj-art-0ab95ae28147400daacc70f68cb58c0f |
| institution | Kabale University |
| issn | 1829-8346 2502-5988 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Universitas Lancang Kuning |
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| series | Jurnal Ilmiah Pertanian |
| spelling | doaj-art-0ab95ae28147400daacc70f68cb58c0f2025-02-07T09:32:23ZengUniversitas Lancang KuningJurnal Ilmiah Pertanian1829-83462502-59882024-03-01211Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchidsYuniati0Mayta Novaliza Isda1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau, Pekanbaru, Indonesia.Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau, Pekanbaru, Indonesia. Grammatophyllum stapeliiflorum, recognized as the melancholic orchid, represents one of the elusive orchid species facing the brink of extinction, thus exacerbating its rarity. Tissue culture technology becomes imperative for the propagation of this orchid species. This study aims to scrutinize the influence of different concentrations of the synthetic growth regulator thidiazuron (TDZ) on distinct basal media types, Murashige Skoog (MS) and Vacin and Went (VW), on the in vitro growth of G. stapeliiflorum orchids. Employing a complete randomized factorial design, the study entails two research factors: TDZ concentration with 4 treatment levels, A0 = 0 mg/L, A1 = 0.25 mg/L, A2 = 0.50 mg/L, and A3 = 0.75 mg/L, and the second factor being the media type, B1 = ½ MS and B2 = ½ VW. Findings indicate significant effects of MS and VW media on the percentage of viable explants, browning, globular forms, and shoots. Interaction between TDZ concentration and media type didn't yield significant effects on each experimental parameter. Treatment with 0.25 and 0.50 mg/L TDZ on ½ VW media demonstrated optimal results for explant growth percentage, browning, and explants in the globular phase, at 97.33%, 2.67%, and 2.67%, respectively. Moreover, treatment with 0.75 mg/L TDZ on ½ VW media resulted in the highest percentage of explant coloration. The combination of TDZ and media has shown the potential to enhance the production efficiency and conservation of this rare orchid species through tissue culture technology. https://dev-journal.unilak.ac.id/index.php/jip/article/view/12829Grammatophyllum stapeliiflorumgrowth regulationin vitroorchid conservationprotocorm |
| spellingShingle | Yuniati Mayta Novaliza Isda Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchids Jurnal Ilmiah Pertanian Grammatophyllum stapeliiflorum growth regulation in vitro orchid conservation protocorm |
| title | Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchids |
| title_full | Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchids |
| title_fullStr | Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchids |
| title_full_unstemmed | Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchids |
| title_short | Combination of thidiazuron and basal media type on optimizing in vitro growth of Grammatophyllum stapeliiflorum orchids |
| title_sort | combination of thidiazuron and basal media type on optimizing in vitro growth of grammatophyllum stapeliiflorum orchids |
| topic | Grammatophyllum stapeliiflorum growth regulation in vitro orchid conservation protocorm |
| url | https://dev-journal.unilak.ac.id/index.php/jip/article/view/12829 |
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