Genome-Wide Characterization and Haplotype Module Stacking Analysis of the <i>KTI</i> Gene Family in Soybean (<i>Glycine max</i> L. Merr.)

Genome-Wide Characterization and Haplotype Module Stacking Analysis of the <i>KTI</i> Gene Family in Soybean (<i>Glycine max</i> L. Merr.)

The Kunitz trypsin inhibitor (<i>KTI</i>) gene family encompasses a category of trypsin inhibitors, and the KTI proteins are important components of the 2S storage protein fraction in soybeans. In this study, fifty members of the <i>GmKTI</i> family were identified in the soy...

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Main Authors: Huilin Tian, Zhanguo Zhang, Shaowei Feng, Jia Song, Xue Han, Xin Chen, Candong Li, Enliang Liu, Linli Xu, Mingliang Yang, Qingshan Chen, Xiaoxia Wu, Zhaoming Qi
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Agronomy
Subjects:
kunitz trypsin inhibitor
seed storage protein content
paralogous genes
haplotype module stacking
soybean
Online Access:https://www.mdpi.com/2073-4395/15/5/1210
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Summary:The Kunitz trypsin inhibitor (<i>KTI</i>) gene family encompasses a category of trypsin inhibitors, and the KTI proteins are important components of the 2S storage protein fraction in soybeans. In this study, fifty members of the <i>GmKTI</i> family were identified in the soybean genome, and their physicochemical properties, domain compositions, phylogenetic relationships, gene structures, and expression patterns were comprehensively analyzed to explore their impact on soybean seed protein content. The results revealed significant gene expansion within the <i>GmKTI</i> family in soybean. The gene structures and conserved motifs of <i>GmKTI</i> members exhibited both regularity and diversity, with distinct expression patterns across different soybean tissues. Haplotype analysis identified 7 <i>GmKTI</i> genes significantly associated with seed storage protein content, and the combination of superior haplotypes was found to enhance seed storage protein content. This is crucial for the improvement of soybean varieties and the enhancement of storage protein content. Additionally, the <i>GmKTI</i> family demonstrated evolutionary conservation, with its functions likely linked to light induction, biotic stress, and growth development. This study characterizes the structure, expression, genomic haplotypes, and molecular features of the soybean KTI domain for the first time, providing a foundation for functional analyses of the <i>GmKTI</i> domain in soybean and other plants.
ISSN:2073-4395

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