Separation of bivalent anti-T cell immunotoxin from Pichia pastoris glycoproteins by borate anion exchange
A major problem encountered in the large-scale purification of the bivalent anti-T cell immunotoxin, A-dmDT390-bisFv(G4S), from Pichia pastoris supernatants was the presence of host glycoproteins exhibiting similar charge, size, and hydrophobicity characteristics. We overcame this problem by employi...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03352pt04 |
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