Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeast

The aim of this study was to assess the relationship between the expression of the CD23 molecule on B-cells and the levels of specific IgE against allergens and molecular components of storage mites (Gly d 2, Lep d 2), dog (Can f 1, Can f 2), cat (Fel d 1), shrimp (Pen m 2), molds (Asp f 6, Mala s 1...

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Main Authors: Jarmila Čelakovská, Petra Boudkova, Eva Cermakova, Ctirad Andrys
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Immunotoxicology
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/1547691X.2025.2531794
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author Jarmila Čelakovská
Petra Boudkova
Eva Cermakova
Ctirad Andrys
author_facet Jarmila Čelakovská
Petra Boudkova
Eva Cermakova
Ctirad Andrys
author_sort Jarmila Čelakovská
collection DOAJ
description The aim of this study was to assess the relationship between the expression of the CD23 molecule on B-cells and the levels of specific IgE against allergens and molecular components of storage mites (Gly d 2, Lep d 2), dog (Can f 1, Can f 2), cat (Fel d 1), shrimp (Pen m 2), molds (Asp f 6, Mala s 11, Alt a 6, Alt a 1, Mala s 6, Cla h), and German cockroach (Bla g 9) in atopic dermatitis (AD) patients (with and without dupilumab therapy). Here, 46 patients with AD were included (26 without dupilumab treatment, 20 with dupilumab treatment). Serum levels of specific IgE were measured using the component-resolved diagnostic microarray ALEX2 Allergy Xplorer, and the expression of the CD23 molecule on B-cells was evaluated using flow cytometry. For statistical analysis, a Spearman’s rank correlation was used. The data indicated there was a higher correlation between CD23 expression on B-cells and specific IgE against molecular components of storage mites Bla g 9 (up to 27%), cat Fel d 1 (22.7%), and allergen extract Cla h (Cladosporium herbarum) up to 38.9% in AD patients treated with dupilumab. These results regarding the higher association suggested a significant role in the non-inflammatory clearance and uptake of these specific IgE antibodies.
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spelling doaj-art-0a8077b2591644269e7a9ae96f1cda4e2025-08-20T03:41:31ZengTaylor & Francis GroupJournal of Immunotoxicology1547-691X1547-69012025-12-0122110.1080/1547691X.2025.2531794Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeastJarmila Čelakovská0Petra Boudkova1Eva Cermakova2Ctirad Andrys3Department of Dermatology and Venereology, Faculty Hospital, Medical Faculty of Charles University, Hradec Králové, Czech RepublicDepartment of Clinical Immunology and Allergy, Faculty Hospital, Medical Faculty of Charles University, Hradec Králové, Czech RepublicDepartment of Medical Biophysics, Medical Faculty of Charles University, Hradec Králové, Czech RepublicDepartment of Clinical Immunology and Allergy, Faculty Hospital, Medical Faculty of Charles University, Hradec Králové, Czech RepublicThe aim of this study was to assess the relationship between the expression of the CD23 molecule on B-cells and the levels of specific IgE against allergens and molecular components of storage mites (Gly d 2, Lep d 2), dog (Can f 1, Can f 2), cat (Fel d 1), shrimp (Pen m 2), molds (Asp f 6, Mala s 11, Alt a 6, Alt a 1, Mala s 6, Cla h), and German cockroach (Bla g 9) in atopic dermatitis (AD) patients (with and without dupilumab therapy). Here, 46 patients with AD were included (26 without dupilumab treatment, 20 with dupilumab treatment). Serum levels of specific IgE were measured using the component-resolved diagnostic microarray ALEX2 Allergy Xplorer, and the expression of the CD23 molecule on B-cells was evaluated using flow cytometry. For statistical analysis, a Spearman’s rank correlation was used. The data indicated there was a higher correlation between CD23 expression on B-cells and specific IgE against molecular components of storage mites Bla g 9 (up to 27%), cat Fel d 1 (22.7%), and allergen extract Cla h (Cladosporium herbarum) up to 38.9% in AD patients treated with dupilumab. These results regarding the higher association suggested a significant role in the non-inflammatory clearance and uptake of these specific IgE antibodies.https://www.tandfonline.com/doi/10.1080/1547691X.2025.2531794Atopic dermatitisCD23 molecule on B lymphocytesspecific IgE against molecular components
spellingShingle Jarmila Čelakovská
Petra Boudkova
Eva Cermakova
Ctirad Andrys
Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeast
Journal of Immunotoxicology
Atopic dermatitis
CD23 molecule on B lymphocytes
specific IgE against molecular components
title Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeast
title_full Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeast
title_fullStr Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeast
title_full_unstemmed Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeast
title_short Interaction between expression of CD23 on B-lymphocytes and level of specific IgE against molecular components of NPC2 family, lipocalins, uteroglobins, and molecular components of molds and yeast
title_sort interaction between expression of cd23 on b lymphocytes and level of specific ige against molecular components of npc2 family lipocalins uteroglobins and molecular components of molds and yeast
topic Atopic dermatitis
CD23 molecule on B lymphocytes
specific IgE against molecular components
url https://www.tandfonline.com/doi/10.1080/1547691X.2025.2531794
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AT evacermakova interactionbetweenexpressionofcd23onblymphocytesandlevelofspecificigeagainstmolecularcomponentsofnpc2familylipocalinsuteroglobinsandmolecularcomponentsofmoldsandyeast
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