Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
<b>Aim:</b> The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freezethawing process.<p> <b>Materials and Methods:</b> Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1133 |
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| Summary: | <b>Aim:</b> The aim of this study was to investigate the effects of
BME on Kangal dog sperm parameters following the freezethawing
process.<p>
<b>Materials and Methods:</b> Ejaculates were collected by digital
manipulation twice a week. Ejaculates divided into six equal
aliquots, and diluted with Tris-based extender (T) containing
5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10%
(TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE +
BME 10% (TEB10), TG and TE. Subsequently, the ejaculates
were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen
(~-196°C).<p>
<b>Results:</b> The extender supplemented with 10% BME + 5% G
resulted in higher progressive motility (23.19%), in comparison
to the extenders supplemented with 5% EG (14.08%).
Better sperm acrosome integrity was achieved with the use
of the extender containing 2.5% BME + 5% G (44.99%), when
compared to the use of the extender supplemented with 5%
G (33.63%) (P<0.05).<p>
<b>Conclusion:</b> BME added to extender, may improve the postthawed
sperm parameters on freezing of Kangal dog semen. |
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| ISSN: | 1309-6958 2146-1953 |