X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT
The kidney’s microstructure, which comprises a highly convoluted tubular and vascular network, can only be partially revealed using classical 2D histology. Considering that the kidney’s microstructure is closely related to its function and is often affected by pathologies, there is a need for powerf...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/2024/3924036 |
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| _version_ | 1832548309558886400 |
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| author | Arne Maes Onno Borgel Clara Braconnier Tim Balcaen Martine Wevers Rebecca Halbgebauer Markus Huber-Lang Greet Kerckhofs |
| author_facet | Arne Maes Onno Borgel Clara Braconnier Tim Balcaen Martine Wevers Rebecca Halbgebauer Markus Huber-Lang Greet Kerckhofs |
| author_sort | Arne Maes |
| collection | DOAJ |
| description | The kidney’s microstructure, which comprises a highly convoluted tubular and vascular network, can only be partially revealed using classical 2D histology. Considering that the kidney’s microstructure is closely related to its function and is often affected by pathologies, there is a need for powerful and high-resolution 3D imaging techniques to visualize the microstructure. Here, we present how cryogenic contrast-enhanced microCT (cryo-CECT) allowed 3D visualization of glomeruli, tubuli, and vasculature. By comparing different contrast-enhancing staining agents and freezing protocols, we found that the preferred sample preparation protocol was the combination of staining with 1:2 hafnium(IV)-substituted Wells-Dawson polyoxometalate and freezing by submersion in isopentane at −78°C. This optimized protocol showed to be highly sensitive, allowing to detect small pathology-induced microstructural changes in a mouse model of mild trauma-related acute kidney injury after thorax trauma and hemorrhagic shock. In summary, we demonstrated that cryo-CECT is an effective 3D histopathological tool that allows to enhance our understanding of kidney tissue microstructure and their related function. |
| format | Article |
| id | doaj-art-0a7614c4cb23476d81d858a5dbb0a8ea |
| institution | Kabale University |
| issn | 1687-4196 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-0a7614c4cb23476d81d858a5dbb0a8ea2025-02-03T06:14:54ZengWileyInternational Journal of Biomedical Imaging1687-41962024-01-01202410.1155/2024/3924036X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCTArne Maes0Onno Borgel1Clara Braconnier2Tim Balcaen3Martine Wevers4Rebecca Halbgebauer5Markus Huber-Lang6Greet Kerckhofs7Department of Materials EngineeringInstitute of Clinical and Experimental Trauma-ImmunologyBiomechanics LabBiomechanics LabDepartment of Materials EngineeringInstitute of Clinical and Experimental Trauma-ImmunologyInstitute of Clinical and Experimental Trauma-ImmunologyDepartment of Materials EngineeringThe kidney’s microstructure, which comprises a highly convoluted tubular and vascular network, can only be partially revealed using classical 2D histology. Considering that the kidney’s microstructure is closely related to its function and is often affected by pathologies, there is a need for powerful and high-resolution 3D imaging techniques to visualize the microstructure. Here, we present how cryogenic contrast-enhanced microCT (cryo-CECT) allowed 3D visualization of glomeruli, tubuli, and vasculature. By comparing different contrast-enhancing staining agents and freezing protocols, we found that the preferred sample preparation protocol was the combination of staining with 1:2 hafnium(IV)-substituted Wells-Dawson polyoxometalate and freezing by submersion in isopentane at −78°C. This optimized protocol showed to be highly sensitive, allowing to detect small pathology-induced microstructural changes in a mouse model of mild trauma-related acute kidney injury after thorax trauma and hemorrhagic shock. In summary, we demonstrated that cryo-CECT is an effective 3D histopathological tool that allows to enhance our understanding of kidney tissue microstructure and their related function.http://dx.doi.org/10.1155/2024/3924036 |
| spellingShingle | Arne Maes Onno Borgel Clara Braconnier Tim Balcaen Martine Wevers Rebecca Halbgebauer Markus Huber-Lang Greet Kerckhofs X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT International Journal of Biomedical Imaging |
| title | X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT |
| title_full | X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT |
| title_fullStr | X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT |
| title_full_unstemmed | X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT |
| title_short | X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT |
| title_sort | x ray based 3d histopathology of the kidney using cryogenic contrast enhanced microct |
| url | http://dx.doi.org/10.1155/2024/3924036 |
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