High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats

IntroductionImmunophenotyping is a powerful tool for grading disease severity, aiding in diagnosis, predicting clinical response, and guiding the development of novel therapeutics.MethodsThis pilot study employs high parameter immunophenotyping panels (15 markers for dog, 12 for cat) and leverages u...

Full description

Saved in:
Bibliographic Details
Main Authors: Erin McDonald, Eric Kehoe, Darcy Deines, Mary McCarthy, Brie Wright, Susan Huse
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Veterinary Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2024.1498964/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832591351436279808
author Erin McDonald
Eric Kehoe
Darcy Deines
Mary McCarthy
Brie Wright
Susan Huse
author_facet Erin McDonald
Eric Kehoe
Darcy Deines
Mary McCarthy
Brie Wright
Susan Huse
author_sort Erin McDonald
collection DOAJ
description IntroductionImmunophenotyping is a powerful tool for grading disease severity, aiding in diagnosis, predicting clinical response, and guiding the development of novel therapeutics.MethodsThis pilot study employs high parameter immunophenotyping panels (15 markers for dog, 12 for cat) and leverages unsupervised clustering to identify immune cell populations. Our analysis uses machine learning and statistical algorithms to perform unsupervised clustering, multiple visualizations, and statistical analysis of high parameter flow cytometry data. This method reduces user bias and precisely identifies cell populations, demonstrating its potential to detect variations and differentiate populations effectively. To enhance our understanding of cat and dog biology and test the unsupervised clustering approach on real-world samples, we performed in-depth profiling of immune cell populations in blood collected from client-owned and laboratory animals [dogs (n = 55) and cats (n = 68)]. These animals were categorized based on pruritic behavior or routine check-ups (non-pruritic controls).ResultsUnsupervised clustering revealed various immune cell populations, including T-cell subsets distinguished by CD62L expression and distinct monocyte subsets. Notably, there were significant differences in monocyte subsets between pruritic and non-pruritic animals. Pruritic dogs and cats showed significant shifts in CD62LHi T-cell subsets compared to non-pruritic controls, with opposite trends observed between pruritic cats and dogs.DiscussionThese findings underscore the importance of advancing veterinary immunophenotyping, expanding our knowledge about marker expression on circulating immune cells and driving progress in understanding veterinary-specific biology and uncovering new insights into various conditions and diseases.
format Article
id doaj-art-0a6e237ae84f485d94ef96d82a0f7d9b
institution Kabale University
issn 2297-1769
language English
publishDate 2025-01-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Veterinary Science
spelling doaj-art-0a6e237ae84f485d94ef96d82a0f7d9b2025-01-22T12:40:43ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.14989641498964High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and catsErin McDonaldEric KehoeDarcy DeinesMary McCarthyBrie WrightSusan HuseIntroductionImmunophenotyping is a powerful tool for grading disease severity, aiding in diagnosis, predicting clinical response, and guiding the development of novel therapeutics.MethodsThis pilot study employs high parameter immunophenotyping panels (15 markers for dog, 12 for cat) and leverages unsupervised clustering to identify immune cell populations. Our analysis uses machine learning and statistical algorithms to perform unsupervised clustering, multiple visualizations, and statistical analysis of high parameter flow cytometry data. This method reduces user bias and precisely identifies cell populations, demonstrating its potential to detect variations and differentiate populations effectively. To enhance our understanding of cat and dog biology and test the unsupervised clustering approach on real-world samples, we performed in-depth profiling of immune cell populations in blood collected from client-owned and laboratory animals [dogs (n = 55) and cats (n = 68)]. These animals were categorized based on pruritic behavior or routine check-ups (non-pruritic controls).ResultsUnsupervised clustering revealed various immune cell populations, including T-cell subsets distinguished by CD62L expression and distinct monocyte subsets. Notably, there were significant differences in monocyte subsets between pruritic and non-pruritic animals. Pruritic dogs and cats showed significant shifts in CD62LHi T-cell subsets compared to non-pruritic controls, with opposite trends observed between pruritic cats and dogs.DiscussionThese findings underscore the importance of advancing veterinary immunophenotyping, expanding our knowledge about marker expression on circulating immune cells and driving progress in understanding veterinary-specific biology and uncovering new insights into various conditions and diseases.https://www.frontiersin.org/articles/10.3389/fvets.2024.1498964/fullflow cytometrypruritisimmunophenotypingmachine learningartificial intelligenceunsupervised clustering
spellingShingle Erin McDonald
Eric Kehoe
Darcy Deines
Mary McCarthy
Brie Wright
Susan Huse
High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats
Frontiers in Veterinary Science
flow cytometry
pruritis
immunophenotyping
machine learning
artificial intelligence
unsupervised clustering
title High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats
title_full High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats
title_fullStr High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats
title_full_unstemmed High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats
title_short High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats
title_sort high parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats
topic flow cytometry
pruritis
immunophenotyping
machine learning
artificial intelligence
unsupervised clustering
url https://www.frontiersin.org/articles/10.3389/fvets.2024.1498964/full
work_keys_str_mv AT erinmcdonald highparameterimmunophenotypingrevealsdistinctimmunecellprofilesinpruriticdogsandcats
AT erickehoe highparameterimmunophenotypingrevealsdistinctimmunecellprofilesinpruriticdogsandcats
AT darcydeines highparameterimmunophenotypingrevealsdistinctimmunecellprofilesinpruriticdogsandcats
AT marymccarthy highparameterimmunophenotypingrevealsdistinctimmunecellprofilesinpruriticdogsandcats
AT briewright highparameterimmunophenotypingrevealsdistinctimmunecellprofilesinpruriticdogsandcats
AT susanhuse highparameterimmunophenotypingrevealsdistinctimmunecellprofilesinpruriticdogsandcats