Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC Method
An effective, simple and reliable simultaneous determination method for four active compounds, mesotrione, benoxacor, atrazine and S-metolachlor, in herbicide formulate was first-time developed and substantiated in current study using reversed-phased high performance liquid chromatography, following...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Centre of Excellence in Analytical Chemsitry
2025-06-01
|
| Series: | Pakistan Journal of Analytical & Environmental Chemistry |
| Subjects: | |
| Online Access: | https://pjaec.pk/index.php/pjaec/article/view/457/369 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849424867822141440 |
|---|---|
| author | Anum Hafeez Sajid Iqbal Iffat Abdul Tawab Khan Qurrat-ul-Ain |
| author_facet | Anum Hafeez Sajid Iqbal Iffat Abdul Tawab Khan Qurrat-ul-Ain |
| author_sort | Anum Hafeez |
| collection | DOAJ |
| description | An effective, simple and reliable simultaneous determination method for four active compounds, mesotrione, benoxacor, atrazine and S-metolachlor, in herbicide formulate was first-time developed and substantiated in current study using reversed-phased high performance liquid chromatography, following ICH guidelines. The chromatographic separation with fine resolution was conducted on a 5 μm C-18 column (L x id, 15 cm x 4.6 mm) using acetonitrile:water 70:30 (v/v) at 1.0 mL/min flow rate and wavelength 260 nm (UV detector). The retention times for mesotrione, atrazine, benoxacor and S-metolachlor were obtained as 1.90, 3.50, 3.95, and 5.10 min, respectively. Limits of detection of mesotrione, atrazine, benoxacor and S-metolachlor were found to be 2.57, 2.27, 1.28, and 1.32 µg/mL, whereas limits of quantitation were 7.80, 6.86, 3.86, and 4.01 µg/mL, respectively. The regression coefficients from calibration curves were ˃ 0.998 for all studied pesticides. The method reproducibility was evaluated as intra-day precision (0.85–1.52 %RSD) and inter-day precision (0.67–1.81 %RSD). The method accuracy was estimated through inter-laboratory comparison among three laboratories. The developed method is quite rapid, inexpensive, robust, precise, accurate, linear and sensitive for the purpose of quality control check of mesotrione, atrazine, benoxacor and S-metolachlor simultaneously in herbicide formulations. |
| format | Article |
| id | doaj-art-0a6ca4d847e94fbe9fc02a70b081bb0b |
| institution | Kabale University |
| issn | 1996-918X 2221-5255 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | National Centre of Excellence in Analytical Chemsitry |
| record_format | Article |
| series | Pakistan Journal of Analytical & Environmental Chemistry |
| spelling | doaj-art-0a6ca4d847e94fbe9fc02a70b081bb0b2025-08-20T03:29:58ZengNational Centre of Excellence in Analytical ChemsitryPakistan Journal of Analytical & Environmental Chemistry1996-918X2221-52552025-06-01260110511610.21743/pjaec/2025.06.09Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC MethodAnum Hafeez0Sajid Iqbal1Iffat Abdul Tawab Khan2Qurrat-ul-Ain3Department of Chemistry, Federal Urdu University of Arts, Science & Technology, Gulshan Campus, Karachi-75300, Pakistan.Jinnah Government Degree College, Nazimabad, Karachi-74600, Pakistan.Department of Chemistry, Federal Urdu University of Arts, Science & Technology, Gulshan Campus, Karachi-75300, Pakistan.Department of Chemistry, Faculty of Science, University of Karachi, Karachi-75270, Pakistan.An effective, simple and reliable simultaneous determination method for four active compounds, mesotrione, benoxacor, atrazine and S-metolachlor, in herbicide formulate was first-time developed and substantiated in current study using reversed-phased high performance liquid chromatography, following ICH guidelines. The chromatographic separation with fine resolution was conducted on a 5 μm C-18 column (L x id, 15 cm x 4.6 mm) using acetonitrile:water 70:30 (v/v) at 1.0 mL/min flow rate and wavelength 260 nm (UV detector). The retention times for mesotrione, atrazine, benoxacor and S-metolachlor were obtained as 1.90, 3.50, 3.95, and 5.10 min, respectively. Limits of detection of mesotrione, atrazine, benoxacor and S-metolachlor were found to be 2.57, 2.27, 1.28, and 1.32 µg/mL, whereas limits of quantitation were 7.80, 6.86, 3.86, and 4.01 µg/mL, respectively. The regression coefficients from calibration curves were ˃ 0.998 for all studied pesticides. The method reproducibility was evaluated as intra-day precision (0.85–1.52 %RSD) and inter-day precision (0.67–1.81 %RSD). The method accuracy was estimated through inter-laboratory comparison among three laboratories. The developed method is quite rapid, inexpensive, robust, precise, accurate, linear and sensitive for the purpose of quality control check of mesotrione, atrazine, benoxacor and S-metolachlor simultaneously in herbicide formulations. https://pjaec.pk/index.php/pjaec/article/view/457/369crop protection agentsliquid chromatographymethod optimizationrobustnessuv detector |
| spellingShingle | Anum Hafeez Sajid Iqbal Iffat Abdul Tawab Khan Qurrat-ul-Ain Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC Method Pakistan Journal of Analytical & Environmental Chemistry crop protection agents liquid chromatography method optimization robustness uv detector |
| title | Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC Method |
| title_full | Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC Method |
| title_fullStr | Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC Method |
| title_full_unstemmed | Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC Method |
| title_short | Efficient Concurrent Analysis of Mesotrione, Atrazine, Benoxacor and S-Metolachlor in Herbicide Formulate: Developing and Validating a Reversed-Phased HPLC Method |
| title_sort | efficient concurrent analysis of mesotrione atrazine benoxacor and s metolachlor in herbicide formulate developing and validating a reversed phased hplc method |
| topic | crop protection agents liquid chromatography method optimization robustness uv detector |
| url | https://pjaec.pk/index.php/pjaec/article/view/457/369 |
| work_keys_str_mv | AT anumhafeez efficientconcurrentanalysisofmesotrioneatrazinebenoxacorandsmetolachlorinherbicideformulatedevelopingandvalidatingareversedphasedhplcmethod AT sajidiqbal efficientconcurrentanalysisofmesotrioneatrazinebenoxacorandsmetolachlorinherbicideformulatedevelopingandvalidatingareversedphasedhplcmethod AT iffatabdultawabkhan efficientconcurrentanalysisofmesotrioneatrazinebenoxacorandsmetolachlorinherbicideformulatedevelopingandvalidatingareversedphasedhplcmethod AT qurratulain efficientconcurrentanalysisofmesotrioneatrazinebenoxacorandsmetolachlorinherbicideformulatedevelopingandvalidatingareversedphasedhplcmethod |