Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells
In this paper, we developed a two-step-induction method of generating functional hair cells from inner ear multipotent cells. Multipotent cells from the inner ear were established and induced initially into progenitor cells committed to the inner ear cell lineage on the poly-L-lysine substratum. Sub...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8197279 |
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| author | Quanwen Liu Yi Shen Jiarong Chen Jie Ding Zihua Tang Cui Zhang Jianling Chen Liang Li Ping Chen Jinfu Wang |
| author_facet | Quanwen Liu Yi Shen Jiarong Chen Jie Ding Zihua Tang Cui Zhang Jianling Chen Liang Li Ping Chen Jinfu Wang |
| author_sort | Quanwen Liu |
| collection | DOAJ |
| description | In this paper, we developed a two-step-induction method of generating functional hair cells from inner ear multipotent cells. Multipotent cells from the inner ear were established and induced initially into progenitor cells committed to the inner ear cell lineage on the poly-L-lysine substratum. Subsequently, the committed progenitor cells were cultured on the mitotically inactivated chicken utricle stromal cells and induced into hair-cell-like cells containing characteristic stereocilia bundles. The hair-cell-like cells exhibited rapid permeation of FM1-43FX. The whole-cell patch-clamp technique was used to measure the membrane currents of cells differentiated for 7 days on chicken utricle stromal cells and analyze the biophysical properties of the hair-cell-like cells by recording membrane properties of cells. The results suggested that the hair-cell-like cells derived from inner ear multipotent cells were functional following differentiation in an enabling environment. |
| format | Article |
| id | doaj-art-0a63e6c2d8fe400bb88f0047168345f5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0a63e6c2d8fe400bb88f0047168345f52025-08-20T03:55:27ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/81972798197279Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent CellsQuanwen Liu0Yi Shen1Jiarong Chen2Jie Ding3Zihua Tang4Cui Zhang5Jianling Chen6Liang Li7Ping Chen8Jinfu Wang9Institute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Epidemiology & Health Statistics, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Development, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaIn this paper, we developed a two-step-induction method of generating functional hair cells from inner ear multipotent cells. Multipotent cells from the inner ear were established and induced initially into progenitor cells committed to the inner ear cell lineage on the poly-L-lysine substratum. Subsequently, the committed progenitor cells were cultured on the mitotically inactivated chicken utricle stromal cells and induced into hair-cell-like cells containing characteristic stereocilia bundles. The hair-cell-like cells exhibited rapid permeation of FM1-43FX. The whole-cell patch-clamp technique was used to measure the membrane currents of cells differentiated for 7 days on chicken utricle stromal cells and analyze the biophysical properties of the hair-cell-like cells by recording membrane properties of cells. The results suggested that the hair-cell-like cells derived from inner ear multipotent cells were functional following differentiation in an enabling environment.http://dx.doi.org/10.1155/2016/8197279 |
| spellingShingle | Quanwen Liu Yi Shen Jiarong Chen Jie Ding Zihua Tang Cui Zhang Jianling Chen Liang Li Ping Chen Jinfu Wang Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells Stem Cells International |
| title | Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells |
| title_full | Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells |
| title_fullStr | Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells |
| title_full_unstemmed | Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells |
| title_short | Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells |
| title_sort | induction of functional hair cell like cells from mouse cochlear multipotent cells |
| url | http://dx.doi.org/10.1155/2016/8197279 |
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